Use of pan-hemoplasma PCR for screening horses highly exposed to tick bites from southern Brazil

Vieira, Thállitha Samih Wischral Jayme; Vidotto, Odilon; Guimarães, Ana Márcia Sá; Santos, Andrea Pires dos; Nascimento, Naíla Cannes do; Finger, Mariane Angélica Pommerening; Filho, Ivan Roque de Barros; Dornbusch, Peterson Triches; Biondo, Alexander Welker; Vieira, Roberval Daiton; Messick, Joanne B.

doi:10.5433/1679-0359.2015v36n1p291

Cited by 5 publications

(3 citation statements)

References 6 publications

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“…Blood samples from 190 carthorses previously surveyed for other pathogens [15,16] [17], the antigen-specific conjugate has been previously validated [18,19] and used for horse samples [3,5,6].…”

Section: Samplesmentioning

confidence: 99%

Ehrlichia sp. infection in carthorses of low-income owners, Southern Brazil

Vieira

Krawczak

et al. 2016

Comparative Immunology, Microbiology and Infectious Diseases

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Although well established in dogs, Ehrlichia sp. infection has been scarcely reported in horses. The aim was to perform a comprehensive serological and molecular survey for the detection of Ehrlichia spp. in carthorses from Southern Brazil. Blood samples from 190 carthorses from Paraná State were sampled. Horses were also tested for Borrelia burgdorferi and Anaplasma phagocytophilum. Anti-Ehrlichia sp. antibodies were detected by a commercial rapid ELISA, and immunofluorescence antibody assays (IFA) with E. chaffeensis and E. canis as crude antigens. The molecular and phylogenetic analysis of Ehrlichia sp. was based on 16S rRNA and dsb genes. A total of 52 (27.4%), 4 (2.1%), and 3 (1.6%) horses were positive for Ehrlichia spp., Anaplasma spp. and Borrelia burgdorferi, respectively, by the commercial rapid ELISA. Thirty-eight (20.0%) and 37 (19.5%) horses showed anti-E. chaffeensis and anti-E. canis antibodies by IFA, respectively. One blood sample that also showed anti-E. chaffeensis antibodies was PCR positive for the 16S rRNA and dsb genes of Ehrlichia spp., showing an identity of>98.0% to the uncultured Ehrlichia sp. previously detected in Brazilian jaguars (Panthera onca). Anti-Ehrlichia sp. antibodies and Ehrlichia DNA were detected in carthorses from Southern Brazil, which may post public health concerns due to intimate contact with low-income owners. This is the first report of a natural infection of this bacteria in horses from South America. Clinical signs and the tick vector remain unknown.

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Section: Samplesmentioning

confidence: 99%

Ehrlichia sp. infection in carthorses of low-income owners, Southern Brazil

Vieira

Krawczak

et al. 2016

Comparative Immunology, Microbiology and Infectious Diseases

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“…Among the potential causes of negative results from tests for hemoplasmas, previous reports have suggested that these include healthy animals [ 17 , 18 ]; low parasitemia [ 19 ]; sample size and type of population [ 17 ]; climatic conditions, presence of the vector and hemoplasma sequestration in tissues [ 20 ]; testing conducted during and immediately after antibiotic treatment [ 21 ]; and use of inappropriate laboratory tests [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

Molecular screening for hemotropic mycoplasmas in captive Barbary sheep (Ammotragus lervia) in southern Brazil

Lc¹,

Vidotto²,

Vm³

et al. 2017

Vet World

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Aim:This study is part of an active surveillance program for monitoring animal health status in endangered species, and was conducted to screen captive Barbary sheep (Ammotragus lervia) for hemoplasma infection.Materials and Methods:A total of 12 blood samples were collected, DNA extracted and further tested by a pan-hemoplasma polymerase chain reaction protocol.Results:Animals were clinically healthy and not infested by ectoparasites. Although housekeeping gene DNA was successfully amplified, all the Barbary sheep samples tested negative for Mycoplasma sp.Conclusion:Notwithstanding the negative results, molecular pathogen surveys on Barbary sheep and other exotic wild mammals may provide insights regarding infection of endangered species caused by captivity stress in association with exposure to new pathogens worldwide.

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“…In these studies, Canditus M. haemobos and M. haemofelis, which are related to cattle and cats, respectively, in Germany (27), M. ovis which is mostly related to sheep in Brazil (30), Mycoplasma ovis-like and Candidatus M. haemocervae were detected in horses from Iran and Nigeria, respectively (28,29). Besides this, in three different studies investigating TBPs in horses in Brazil, the presence of T. equi and B. caballi was reported, but hemotropic mycoplasma could not be detected in the horses (78)(79)(80). Additionally, some researchers have stated that hemoplasma infections may be accidental or an uncommon disease in horses (78).…”

Section: Discussionmentioning

confidence: 99%

Survey of tick-borne pathogens in grazing horses in Kyrgyzstan: phylogenetic analysis, genetic diversity, and prevalence of Theileria equi

Altay,

Erol,

Sahin

et al. 2024

Front. Vet. Sci.

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IntroductionTick-borne pathogens (TBP) are an important group of organisms that can affect animals and humans all over the world. Equine piroplasmosis (EP), caused by Theileria equi and Babesia caballi, is considered one of the most important tick-borne diseases and can cause significant clinical symptoms and mortality in horses. Moreover, EP plays a restrictive role in international horse traditions and transportation. Although these species can cause similar symptoms, there are different 18S rRNA genotypes of T. equi (five genotypes) and B. caballi (three genotypes). Besides piroplasma species, Anaplasma and hemotropic mycoplasmas (HM) are known as other important tick-borne pathogens reported in horses.MethodsIn this study, we investigated the presence, prevalence, genetic diversity, and phylogenetic analyses of TBPs using PCRs and DNA sequencing in grazing horses in Kyrgyzstan. For these purposes, a total of 311 blood samples were collected from Chuy, Issyk-Kul, Naryn, Osh, Talas, and Jalal-Abad.ResultsDNA amplification of TBP revealed that 23 (7.40%) out of 311 samples were found to be positive for T. equi. However, B. caballi, HM, A. phagocytophilum, and A. capra were not detected in this study. The infection rate of T. equi was higher in males (8.11%) than in females (6.35%) (p=0.2880) and in those older than 5 years (9.02%) than in the 1-4 age group (6.35%) (p=0.1950). Phylogenetic analysis of 18S rRNA revealed that A and E genotypes of T. equi have circulated in grazing horses in Kyrgyzstan.DiscussionInformation about the genetic diversity of T. equi is important for understanding the population dynamics of the species and developing effective control strategies against this pathogen. This is the first molecular investigation of A. capra in horses in Kyrgyzstan. Although this pathogen has been detected in different hosts in Kyrgyzstan, it was not detected in this study. However, considering the wide host spectrum of A. capra, it is thought that more large-scale studies are needed to understand the effect of horses on the epidemiology of this pathogen.

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Use of pan-hemoplasma PCR for screening horses highly exposed to tick bites from southern Brazil

Cited by 5 publications

References 6 publications

Ehrlichia sp. infection in carthorses of low-income owners, Southern Brazil

Ehrlichia sp. infection in carthorses of low-income owners, Southern Brazil

Molecular screening for hemotropic mycoplasmas in captive Barbary sheep (Ammotragus lervia) in southern Brazil

Survey of tick-borne pathogens in grazing horses in Kyrgyzstan: phylogenetic analysis, genetic diversity, and prevalence of Theileria equi

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