Use of Papergrams in the Study of the Urinary Excretion of Radioactive Sulfur Compounds

Biochemical Analysis

“…Radioactive carbon or sulfur incorporated in the amino acids has also allowed the estimation of amino acids in the spots with reasonable success (25,108,169). Similar use of tracer sulfur was made by Tomarelli and Florey (300) in studying urinary sulfur compounds after separation on paper. Tracer iodine was similarly used by Taurog, Tong, and Chaikoff (290) to evaluate the fractions of thyroid iodine.…”

Paper Chromatography

“…Radioactive tracers and paper chromatography7 were used (190) to show that cystine and methionine are important sulfur compounds in alfalfa leaf proteins. Radiomethionine was administered orally7 to a rat, followed by the injection of benzene to study7 the benzene detoxification products (196). The paper chromatogram was read automatically7 by7 synchronizing a constant-speed motor pulling the paper strip slowly under the Geiger counter, with a tape recorder whose pen was motivated by counting impulses from the scaler.…”

“…However, even though the majority of workers employ this expression as an accurate designation of what happens, other investigators wonder if frequent absorption of solvent by the cellulose followed by elution does not occur during this process. Therefore "papyrography" (papyrus; paper, and grapho: write) was next suggested (186) as a more apt phrase; "papergram" (3,196) instead of "paper chromatogram" designated the completed result.…”

“…Radioactive sulfur (190,196), iodine (61,188,189), and carbon (11,63) are first separated from their various compounds on paper and then their positions are determined and quantitatively estimated by the use of Geiger-Mtiller counters, or by laying the paper chromatogram on a photographic plate, when a radioautograph results.…”

Chromatographic Separations

“…This use of reagents in paper is akin to the sensitive spot testing technique that has been widely employed by Feigl (32, 33, 62, 53). As in columns, radioactive substances in paper chromatograms can be located with photographic film or with counters (44,55,115,131). Polarographic and enzymatic reactions may also be applied to solutes separated in paper (67).…”