Use of Pathogen-Specific Antibody Biomarkers to Estimate Waterborne Infections in Population-Based Settings

Exum, Natalie G.; Pisanic, Nora; Granger, Douglas A.; Schwab, Kellogg J.; Detrick, Barbara; Kosek, Margaret; Egorov, Andrey I.; Griffin, Shannon M.; Heaney, Christopher D.

doi:10.1007/s40572-016-0096-x

Cited by 24 publications

(14 citation statements)

References 154 publications

(119 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar efforts could augment burden of disease estimates for enteric pathogens, NTDs, and other pathogens that have well-defined antigen targets but lack population-based information about incidence. For example, including antibody responses to diverse enteric pathogens in population-based surveys could improve burden of disease estimates and provide an objective indicator through which we could view progress toward achieving related United Nations sustainable development goals 3.3 (improving health and well-being target 3, which includes reducing waterborne diseases) and 6 (universal access to clean water and sanitation) ( 11 , 29 ).…”

Section: Synergy Through Integrated Serosurveillancementioning

confidence: 99%

Integrated Serologic Surveillance of Population Immunity and Disease Transmission

Arnold¹,

Scobie²,

Priest³

et al. 2018

Emerg. Infect. Dis.

103

View full text Add to dashboard Cite

Antibodies are unique among biomarkers in their ability to identify persons with protective immunity to vaccine-preventable diseases and to measure past exposure to diverse pathogens. Most infectious disease surveillance maintains a single-disease focus, but broader testing of existing serologic surveys with multiplex antibody assays would create new opportunities for integrated surveillance. In this perspective, we highlight multiple areas for potential synergy where integrated surveillance could add more value to public health efforts than the current trend of independent disease monitoring through vertical programs. We describe innovations in laboratory and data science that should accelerate integration and identify remaining challenges with respect to specimen collection, testing, and analysis. Throughout, we illustrate how information generated through integrated surveillance platforms can create new opportunities to more quickly and precisely identify global health program gaps that range from undervaccination to emerging pathogens to multilayered health disparities that span diverse communicable diseases.

show abstract

Section: Synergy Through Integrated Serosurveillancementioning

confidence: 99%

Integrated Serologic Surveillance of Population Immunity and Disease Transmission

Arnold¹,

Scobie²,

Priest³

et al. 2018

Emerg. Infect. Dis.

103

View full text Add to dashboard Cite

show abstract

“…Serological measures have long been used as either correlates of protection induced by a wide range of licensed vaccines targeting pathogens such as yellow fever, tetanus, polio, hepatitis A and B, measles, pertussis, rubella (reviewed in [1]), or as markers of exposure to a variety of pathogens [2]. Testing sera from preclinical and clinical studies has also been used to determine the potency of vaccine formulations as well as their potential to induce cross-species or cross-serotype reactive antibodies.…”

Section: Introductionmentioning

confidence: 99%

Comparison of ELISA with electro-chemiluminescence technology for the qualitative and quantitative assessment of serological responses to vaccination

Bolton¹,

Chaudhury

Dutta³

et al. 2020

Malar J

View full text Add to dashboard Cite

Background: Profiling immune responses induced by either infection or vaccination can provide insight into identification of correlates of protection. Furthermore, profiling of serological responses can be used to identify biomarkers indicative of exposure to pathogens. Conducting such immune surveillance requires readout methods that are highthroughput, robust, and require small sample volumes. While the enzyme-linked immunosorbent assay (ELISA) is the classical readout method for assessing serological responses, the advent of multiplex assays has significantly increased the throughput and capacity for immunoprofiling. This report describes the development and assay performance (sensitivity, linearity of detection, requirement for multiple dilutions for each sample, intra-and inter-assay variability) of an electro-chemiluminescence (ECLIA)-based multiplex assay. Methods:The current study describes the development of a multiplex ECLIA-based assay and characterizes the sensitivity, linear range, and inter-and intra-assay variability of the ECLIA platform and its agreement with the traditional ELISA. Special emphasis was placed on potential antigenic competition when testing closely related antigens in the multiplex format. Results:Multiplexing of antigens in ECLIA provides significant practical benefits in terms of reducing sample volume requirements and experimental time. Beyond the practical advantages of multiplexing, the ECLIA provides superior assay performance when compared to the ELISA. Not only does ECLIA show good agreement with the ELISA assay, but the linear range of ECLIA is also sufficiently wide to permit single-dilution measurements of concentration without the need to do serial dilutions. The lack of antigenic competition allows the simultaneous testing of closely related antigens, such as plate antigens representing different alleles of the same protein, which can inform about cross-reactivities-or lack thereof-of serological responses.

show abstract

“…Future directions for this research could include measuring IgA in serum in parallel to compare it to salivary findings, and sampling the same children at multiple time points to analyze changes in IgA over time, in a setting where infections with enteric pathogens are common. In this study we were also limited by testing for non pathogen-specific SIgA, and a next step for this research could include the exploration of pathogen-specific salivary SIgA as a biomarker to estimate specific infections [49]. Comparing pathogen-specific antibody responses in saliva to detection of those pathogens in matched stool samples is a research need that has also been outlined by researchers at the US Environmental Protection Agency (EPA) [50].…”

Section: Discussionmentioning

confidence: 99%

Child Salivary SIgA and Its Relationship to Enteric Infections and EED Biomarkers in Maputo, Mozambique

Goddard

Knee

Sumner

et al. 2020

IJERPH

View full text Add to dashboard Cite

Characterizing child immunological responses to enteric infections with antibody detection in serum can be challenging in resource-constrained field settings, because sample collection requires trained individuals and its invasive procedure may lead to low response rates, especially among children. Saliva may present a promising non-invasive alternative. The objectives of this research were to compare salivary antibody levels in children to enteric infections and biomarkers of environmental enteric dysfunction (EED). We collected saliva samples from children aged one to six years enrolled in a sanitation trial in Maputo, Mozambique, and characterized salivary secretory immunoglobulin A (SIgA) concentrations with enzyme-linked immunosorbent assays. We used multilevel linear models to analyze cross-sectional associations between salivary SIgA and the number of concurrent enteric pathogen infections, as well as EED biomarkers in matched stool samples. Median salivary SIgA concentrations in this study population were 54 μg/mL (inter-quartile range (IQR): 34, 85 μg/mL), and SIgA levels were similar between children of different ages. SIgA was lower in children experiencing a higher number of concurrent infections −0.04 log μg/mL (95% confidence interval (CI): −0.08 to −0.005 log μg/mL), but was not associated with any of the included EED biomarkers. Contrary to evidence from high-income countries that suggests salivary SIgA increases rapidly with age in young children, the high prevalence of enteric infections may have led to a suppression of immunological development in this study sample and could in part explain the similar SIgA levels between children of different ages.

show abstract

Use of Pathogen-Specific Antibody Biomarkers to Estimate Waterborne Infections in Population-Based Settings

Cited by 24 publications

References 154 publications

Integrated Serologic Surveillance of Population Immunity and Disease Transmission

Integrated Serologic Surveillance of Population Immunity and Disease Transmission

Comparison of ELISA with electro-chemiluminescence technology for the qualitative and quantitative assessment of serological responses to vaccination

Child Salivary SIgA and Its Relationship to Enteric Infections and EED Biomarkers in Maputo, Mozambique

Contact Info

Product

Resources

About