Use of PCR and culture to detect Helicobacter pylori in naturally infected cats following triple antimicrobial therapy

Abstract

Helicobacter pylori causes gastritis and peptic ulcers and is linked to gastric cancer. Domestic cats from a commercial source were found to be naturally infected with H. pylori, and studies were undertaken to eradicate H. pylori from infected cats by using triple antimicrobial therapy. Eight cats infected with H. pylori were used in the study. Six cats received a 21-day course of oral amoxicillin, metronidazole, and omeprazole, and two cats served as controls. Two weeks and 4 weeks posttreatment (p.t.), all six treated cats were negative at several sites (saliva, gastric juice, and gastric mucosa) for H. pylori by culture. However, as determined by PCR with primers specific for the 26-kDa product, the majority of cats at 2 and 4 weeks p.t. had gastric fluid samples which were positive for H. pylori and three of three cats at 2 weeks p.t. had dental plaque which was positive for H. pylori. At 6 weeks p.t., all six cats had H. pylori-negative cultures for samples from several gastric sites taken at necropsy, and only one cat had H. pylori cultured from gastric juice. PCR analysis revealed that five of six cats had H. pylori DNA amplification products from plaque, saliva, and/or gastric fluid samples. Negative bacterial cultures for cats for which there was demonstrable PCR amplification of H. pylori DNA may reflect the inability of in vitro culture techniques to isolate small numbers of H. pylori organisms, focal colonization at sites not cultured, or a failure of the antibiotics to successfully eradicate H. pylori from extragastric sites which allowed subsequent recolonization of the stomach after cessation of therapy. Alternatively, the treatment strategy may have induced in vivo viable but nonculturable coccoid forms of H. pylori. The H. pylori cat model should allow further studies to test these hypotheses as well as the efficacies of other combined therapeutic regimens. Also, because 100% of these cats were naturally infected with H.pylori, this model should prove useful in exploring mechanisms whereby human populations in underdeveloped countries, which have H. pylori infection rates approaching 100%, have a high rate of recurrence of H. pylori infection after use of prescribed antibiotic therapies that successfully eradicate H. pylori in individuals in developed countries.

Helicobacter pyloriIs Not Eradicated after Triple Therapy: A Nested PCR Based Study

“…In an animal model, Cellini et al (1994) demonstrated that up to 3 months after inoculation viable but not culturable forms of H. pylori could still be detected in the mouse stomach [28]. A few studies [29, 30] were carried out to evaluate triple therapy comparing PCR with culture. However no report has included nested PCR.…”

Rol de las formas cocoides de Helicobacter pylori en la infección y la recrudescencia

“…Perkins et al observaron, en gatos infectados de forma natural por H. pylori, que 6 semanas después del tratamiento de erradicación, el análisis del jugo gástrico era positivo para H. pylori en un solo gato, pero mediante la reacción en cadena de la polimerasa se identificó la presencia de material genético de H. pylori en todos los gatos tratados. Las lesiones histológicas eran compatibles con gastritis crónica con la presencia de folículos linfoides, aunque los bacilos de H. pylori fueron detectados en un solo gato 48 .…”

Biology and Diseases of Cats

“…Helicobacter felis is a naturally occurring pathogen in cats that appears to be prevalent in some colonies, but its prevalence or significance as an agent of clinical diseases in the general cat population is not clear (Lee et al, 1988;Perkins et al, 1996). Helicobacter felis is a naturally occurring pathogen in cats that appears to be prevalent in some colonies, but its prevalence or significance as an agent of clinical diseases in the general cat population is not clear (Lee et al, 1988;Perkins et al, 1996).…”

“…Helicobacter felis is a naturally occurring pathogen in cats that appears to be prevalent in some colonies, but its prevalence or significance as an agent of clinical diseases in the general cat population is not clear (Lee et al, 1988;Perkins et al, 1996). In addition to H. felis infection, cats appear also to be naturally infected with H. pylori, raising the possibility that domestic cats could serve as a reservoir for this human pathogen (Perkins et al, 1996). In addition to H. felis infection, cats appear also to be naturally infected with H. pylori, raising the possibility that domestic cats could serve as a reservoir for this human pathogen (Perkins et al, 1996).…”