Use of PCR-restriction fragment length polymorphism for the identification of zoonotic mycobacteriosis in zebrafish caused by Mycobacterium abscessus and Mycobacterium chelonae

Seok, Seung Hyeok; Koo, Hye Cheong; Kasuga, Asako; Kim, Yeun; Lee, Eun Gae; Lee, Hyeyoung; Park, Jong Hwan; Baek, Min Won; Lee, Hui Young; Kim, Dong Jae; Lee, Byeung Hee; Lee, Yong Soon; Cho, Sang Nae; Park, Jae Hak

doi:10.1016/j.vetmic.2005.12.006

Cited by 8 publications

(5 citation statements)

References 18 publications

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“…The sensory feedback provided by detection of longitudinal and lateral bending of the tail could influence the shape of the spine. Another possibility is that CSF-cNs may regulate responses to inflammatory molecules in the CSF, as bacterial infection leads to spinal defects similar to those seen in adult pkd2l1 mutants 43 . How Pkd2l1-dependent activity in CSF-cNs contributes to defects in spine morphogenesis remains an area for future investigation.…”

Section: Discussionmentioning

confidence: 99%

Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature

et al. 2018

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Defects in cerebrospinal fluid (CSF) flow may contribute to idiopathic scoliosis. However, the mechanisms underlying detection of CSF flow in the central canal of the spinal cord are unknown. Here we demonstrate that CSF flows bidirectionally along the antero-posterior axis in the central canal of zebrafish embryos. In the cfap298tm304 mutant, reduction of cilia motility slows transport posteriorly down the central canal and abolishes spontaneous activity of CSF-contacting neurons (CSF-cNs). Loss of the sensory Pkd2l1 channel nearly abolishes CSF-cN calcium activity and single channel opening. Recording from isolated CSF-cNs in vitro, we show that CSF-cNs are mechanosensory and require Pkd2l1 to respond to pressure. Additionally, adult pkd2l1 mutant zebrafish develop an exaggerated spine curvature, reminiscent of kyphosis in humans. These results indicate that CSF-cNs are mechanosensory cells whose Pkd2l1-driven spontaneous activity reflects CSF flow in vivo. Furthermore, Pkd2l1 in CSF-cNs contributes to maintenance of natural curvature of the spine.

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Section: Discussionmentioning

confidence: 99%

Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature

et al. 2018

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“…The sensory feedback provided by detection of longitudinal and lateral bending of the tail could influence the shape of the spine. Another possibility is that CSF-cNs may regulate responses to inflammatory molecules in the CSF, as bacterial infection leads to spinal defects similar to those seen in adult pkd2l1 mutants 41 . How Pkd2l1-dependent activity in CSF-cNs contributes to defects in spine morphogenesis remains an area for future investigation.…”

Section: Discussionmentioning

confidence: 99%

Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature

Sternberg

Prendergast

Brosse

et al. 2018

Preprint

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Flow of cerebrospinal fluid (CSF) may contribute to spine morphogenesis, as mutations affecting both cilia motility and CSF flow lead to scoliosis 1 . However, the mechanisms underlying detection of the CSF flow in the central canal of the spinal cord remain elusive. Here we used full-field optical coherence tomography (FF-OCT) and bead tracking to demonstrate that CSF flows bidirectionally along the antero-posterior axis in the central canal of zebrafish embryos. In the zebrafish mutant cfap298 tm304 , previously known as kurly, reduction of cilia motility slows transport down the length of central canal. To investigate downstream mechanisms that could transduce CSF flow, we performed calcium imaging in sensory neurons contacting the CSF (CSF-cNs) and found that disruption in cilia motility impaired the activity of CSF-cNs. CSF-cNs across species express the transient receptor potential channel PKD2L1, also known as TRPP3, which contributes to CSF-cN chemosensory properties. Using calcium imaging and whole-cell patch clamp recordings, we found that the loss of the Pkd2l1 channel in pkd2l1 mutant embryos also abolished CSF-cN activity. Whole-cell recordings further demonstrated that opening of a single channel is sufficient to trigger action potentials in wild type CSF-cNs. Recording from isolated cells in vitro, we showed that CSF-cNs are mechanosensory cells that respond to pressure in a Pkd2l1-dependent manner. Interestingly, adult pkd2l1 mutant zebrafish develop an exaggerated spine curvature, reminiscent of kyphosis in humans. Our study indicates that CSF-cNs are mechanosensory cells whose spontaneous activity reflects CSF flow in vivo. Furthermore, Pkd2l1 in CSF-cNs contributes to the maintenance of the natural curvature of the spine.

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“…Aquarium fish are particularly susceptible to mycobacterial infections (Pate, Jenčič, Žolnir‐Dovč & Ocepek 2005; Zanoni, Florio, Fioravanti, Rossi & Prearo 2008) and mycobacteria have been identified as significant pathogens of zebrafish, Danio rerio (Hamilton) (Astrofsky, Schrenzel, Bullis, Smolowitz & Fox 2000; Kent, Whipps, Matthews, Florio, Watral, Bishop‐Stewart, Poort & Bermudez 2004; Seok, Koo, Kasuga, Kim, Lee, Lee, Park, Baek, Lee, Kim, Lee, Lee, Cho & Park 2006; Whipps, Dougan & Kent 2007). Zebrafish are excellent hosts for these bacteria and have been increasingly used to study the pathogenesis of mycobacteriosis (Prouty, Correa, Barker, Jagadeeswaran & Klose 2003; Tobin & Ramakrishnan 2008).…”

Section: Introductionmentioning

confidence: 99%

Husbandry stress exacerbates mycobacterial infections in adult zebrafish, Danio rerio (Hamilton)

Ramsay¹,

Watral²,

Schreck³

et al. 2009

Journal of Fish Diseases

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Mycobacteria are significant pathogens of laboratory zebrafish, Danio rerio (Hamilton). Stress is often implicated in clinical disease and morbidity associated with mycobacterial infections but has yet to be examined with zebrafish. The aim of this study was to examine the effects of husbandry stressors on zebrafish infected with mycobacteria. Adult zebrafish were exposed to Mycobacterium marinum or Mycobacterium chelonae, two species that have been associated with disease in zebrafish. Infected fish and controls were then subjected to chronic crowding and handling stressors and examined over an 8-week period. Whole-body cortisol was significantly elevated in stressed fish compared to non-stressed fish. Fish infected with M. marinum ATCC 927 and subjected to husbandry stressors had 14% cumulative mortality while no mortality occurred among infected fish not subjected to husbandry stressors. Stressed fish, infected with M. chelonae H1E2 from zebrafish, were 15-fold more likely to be infected than non-stressed fish at week 8 post-injection. Sub-acute, diffuse infections were more common among stressed fish infected with M. marinum or M. chelonae than non-stressed fish. This is the first study to demonstrate an effect of stress and elevated cortisol on the morbidity, prevalence, clinical disease and histological presentation associated with mycobacterial infections in zebrafish. Minimizing husbandry stress may be effective at reducing the severity of outbreaks of clinical mycobacteriosis in zebrafish facilities.

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Use of PCR-restriction fragment length polymorphism for the identification of zoonotic mycobacteriosis in zebrafish caused by Mycobacterium abscessus and Mycobacterium chelonae

Cited by 8 publications

References 18 publications

Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature

Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature

Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature

Husbandry stress exacerbates mycobacterial infections in adult zebrafish, Danio rerio (Hamilton)

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