Use of Periodic Acid-Schiff Stain in Identification of Pathogenic Fungi in Tissues

Starr, Grier F.; Dawe, Clyde J.; Weed, Lyle A.

doi:10.1093/ajcp/25.1.76

Cited by 17 publications

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“…Although typical forms of the pathogenic yeasts are usually separable, it is often difficult to identify them specifically or to differentiate them from artifacts. Many reports, for example, of ambiguous large forms of H. capsulatum and small forms of Blastomyces dermatitidis have appeared, as have instances of proved or possible dual infection (Binford, 1955;Weed, 1955;Starr et al, 1955;Moore, 1955). Histoplasma duboisii characteristically produces forins similar to those of B. dermatitidis both in tissue and in culture, as do some strains of H. capsulatum to a lesser extent (Drouhet and Schwarz, 1956).…”

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“…Histoplasma duboisii characteristically produces forins similar to those of B. dermatitidis both in tissue and in culture, as do some strains of H. capsulatum to a lesser extent (Drouhet and Schwarz, 1956). The latter may also be simulated by Cryptococcus neoformans (Weed, 1955;Baker, 1957;Seabury, 1958), Sporotrichum schenckii (Kligman and l3aldridge, 1951), Torulopsis glabrata (Lopez Fernandez, 1952), and certain protozoa, particularly Leishmania donovani (Starr et al, 1955). The periodic acid-Schiff stain (Pillsbury and Kligman, 1951) is an excellent tool for detecting fungi in tissues, but often is less satisfactory than the routine hematoxylin and eosin for species identification.…”

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FLUORESCENT STAINING OFHISTOPLASMA CAPSULATUM

Gordon

1959

J Bacteriol

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In an earlier paper from this laboratory (Gordon, 1958) it was demonstrated that fluorescein-labeled specific antiserum employed as a cellular stain will differentiate Candida albicans and Candida tropicalis from other yeasts and yeastlike organisms, including the remaining members of the genus Candida. The present report describes the preparation and use of a similar reagent for detection and ident fication of the yeast phase of Histoplasma capsulatum, which must be differentiated from morphologically similar fungi and protozoa that invade the human body.

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FLUORESCENT STAINING OFHISTOPLASMA CAPSULATUM

Gordon

1959

J Bacteriol

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“…The PAS staining method is more sensitive than haematoxylin and eosin sections (poor color contrast with their surroundings) or cultural methods in tissue sections [12]. Increased visibility in PAS is related to the specific site in the candida cell wall, which takes up brilliant magenta color due to rich glycogen content.…”

Section: Annals Of Clinical and Laboratory Research Issn 2386-5180mentioning

confidence: 99%

“…This was similar to the observations of Almeida et al [15] who inferred that with respect to evaluation of morphological identification of bacteria and fungus, PAS gave excellent staining of fungi but weak staining of bacteria whereas PAP produced good staining of bacteria and weak staining of fungi, whereas thus PAS identified. Starr et al [12] categorized PAS method as one with reliable exclusion-potential as it demonstrated only the fungal agents. Thus this identified fungus in precision compared to Papanicolaou stain.…”

Section: Annals Of Clinical and Laboratory Research Issn 2386-5180mentioning

confidence: 99%

“…In one case, all the cells uniformly took up PAS hindering the diagnosis although candida could be well delineated by morphology. Starr et al [12] describes Artefacts as constant problem encountered during search for fungi in his study. It was largely reflective of the lack of specificity of the PAS stain as it mainly works on contrast method and is not a specific histochemical procedure.…”

Section: Annals Of Clinical and Laboratory Research Issn 2386-5180mentioning

confidence: 99%

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Destained Cervical Smears in Diagnosing Candidiasis Using Periodic Acid Stiff Stain

Sundar¹

2018

Ann Clin Lab Res

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Background: Periodic acid Schiff plays a major role in conforming candida infection. Major drawback in use of this stain in cytology is highly variable sample quality among slides. Typically, use of this stain is done after review of routine Papanicolaou stained smear and thus requires additional unstained sample. If the additional sample lacks representative material, false negative or inconclusive results may occur.Objective: To use the destained cervical smears for application of periodic acid Schiff stain and evaluate the slides qualitatively to determine usefulness of destained cytology specimens in diagnosing candidiasis.Method: Twelve routine Papanicolaou stained cervical cytology smears positive for candida organisms were collected from the Cytopathology laboratory of university medical hospital. Cells in a minimum of 4 fields were photographed under 10x and 40x magnification. These slides were then destained using different concentrations of acid alcohol followed by restaining with Periodic Acid Schiff. The same fields were Rephotographed under 10x and 40x magnification and an observer evaluated the slides qualitatively in comparison with original Papanicolaou smear. The collected data were tabulated and analyzed using SPSS software.Result: Destaining was achieved using all three concentrations (1%, 5%, and 10%) of acid alcohol. But Optimum destaining was achieved using 5% acid alcohol. Periodic acid Schiff independent of destaining process demonstrated candida clearly in destained slides. Staining intensity, background, cell morphology and nuclear counter staining results similar to Papanicolaou stain. Conclusion:Destaining does not appear to affect the ability of Periodic acid Schiff stain to exhibit candida. Thus destained cytology slides prove to be a reliable option in suspected candida cases when few slides are available or only stained slides are there.

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Disseminated Geotrichosis

Chang¹,

Buerger²

1964

Arch Intern Med

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Use of Periodic Acid-Schiff Stain in Identification of Pathogenic Fungi in Tissues

Cited by 17 publications

References 0 publications

FLUORESCENT STAINING OFHISTOPLASMA CAPSULATUM

FLUORESCENT STAINING OFHISTOPLASMA CAPSULATUM

Destained Cervical Smears in Diagnosing Candidiasis Using Periodic Acid Stiff Stain

Disseminated Geotrichosis

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