Use of phosphomannose isomerase-based selection system for Agrobacterium-mediated transformation of tomato and potato

Břı́za, J.; Pavingerová, D.; Přikrylová, P.; Gazdová, J.; Vlasák, Josef; Niedermeierová, H.

doi:10.1007/s10535-008-0090-8

Cited by 28 publications

(6 citation statements)

References 45 publications

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“…This system is influenced by a number of factors such as bacterial strains, plasmids, tissue culture environment, media for explant culture, co-cultivation duration, acetosyringone (AS), explant wounding, selective marker and vector and competency of target plant tissues for infection (Li et al 1997, Salas et al 2001, Zambre et al 2003, Yu et al 2002, Olhoft et al 2003, Wu et al 2003, Cheng et al 2004, Bříza et al 2008, Cho et al 2008. Unlike the role of bacterial factors, the host factors in transformation process has remained obscure for nearly a century, and only recently have we begun to understand how Agrobacterium hijacks host factors and cellular processes during transformation process , Lacroix et al 2005, Tao et al 2004, Zhu et al 2003, Roberts et al 2003, Ditt et al 2006, Anand et al 2007, Loyter et al 2005, Baek and Shapleigh 2005.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated genetic transformation of plants: The role of host

Karami¹,

Esna-Ashari²,

Kurdistani³

et al. 2009

Biologia plant.

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Agrobacterium-mediated genetic transformation is the most widely used technology to obtain overexpression of recombinant proteins in plants. Molecular events that occur within Agrobacterium during interactions with host plants have been studied extensively, and now we have a reasonable understanding the key factors involved in the regulation of T-DNA nuclear import and genomic integration. By contrast, very little is known about the events that take place in the host cells during genetic transformation by Agrobacterium. Here, we describe the plant-related factors including genotype, genes, proteins, competency of target tissues and phenolic compounds that participate in Agrobacteriummediated genetic transformation and discuss their possible roles in this process. Because Agrobacterium probably adapts existing cellular processes for its life cycle, identifying the processes in host cells during Agrobacterium infection might contribute to better understanding of basic biological processes as cell communication, intracellular transport and DNA repair and recombination as well as to expanding the host range of Agrobacterium as a genetic engineering tool.

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Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated genetic transformation of plants: The role of host

Karami¹,

Esna-Ashari²,

Kurdistani³

et al. 2009

Biologia plant.

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“…Addition of phenolic compounds, acetosyringone to the co-cultivation has also been reported to increase the transformation frequency (Katia et al 1993, Cortina andCulianez-Macia 2004). Moreover, different selection markers such as bar gene and phosphomannose isomerase (pmi) gene has also been used for increasing the transformation efficiency of tomato and other important vegetable crops (Cho et al 2008, Bříza et al 2008.…”

mentioning

confidence: 99%

Efficient production of transgenic tomatoes via Agrobacterium-mediated transformation

Kaur¹,

Bansal²

2010

Biologia plant.

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Cotyledonary leaves of 9-d-old tomato (Lycopersicon esculentum Mill.) were co-cultivated with Agrobacterium tumefaciens GV 3101 harboring binary vector pBI101 containing kanamycin resistance gene (npt II) as selection marker. Murashige and Skoog (MS) inorganic salts with Gamborg's B5 vitamins supplemented with optimized concentrations of zeatin riboside and indole-acetic acid resulted in enhanced regeneration efficiency. Under optimized conditions of plant regeneration, transformation frequency in cvs. Pusa Ruby, Pusa Uphar and DT-39 was greater than 37 %. Transformed shoots were selected on kanamycin medium and the presence of the transgene in the primary transformants was confirmed by PCR. Integration of the npt II gene in the tomato genome was further confirmed by Southern blot analysis. RT-PCR analysis using neomycin phospho-transferase (npt II) gene-specific primers confirmed the expression of the transgene in transgenic plants. Transformed plants were successfully transferred to phytotron, where these plants grew to maturity and produced flowers and fruits.Additional key words: cotyledonary leaf explant, genetic transformation, Lycopersicon esculentum, zeatin riboside.

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“…Most initial transformation studies in tomato used neomycin phosphotransferase ( NPT II conferring resistance to kanamycin) and GUS as reporter genes to establish adequate protocols (Table 1). In later studies, other genes such as hygromycin phosphotransferase ( HPT ) gene (Chaudhry and Rashid, 2010), bialaphos resistance ( bar ) gene (Abu‐El‐Heba et al, 2008; Khoudi et al, 2009), and phosphomannose isomerase ( pmi ) gene (Briza et al, 2008) have also been used for optimizing transformation in tomato. For removal of Agrobacterium contamination during subculture of transgenic tissues and shoots, cefotaxime has been most commonly used, although it has also been reported that ticarcillin/potassium clavulanate was superior for removing Agrobacterium contamination and enhancing transformation (Ling et al, 1998).…”

Section: Strategies Employed To Enhance Abiotic Stress Tolerance In Tmentioning

confidence: 99%

An Update on Biotechnological Approaches for Improving Abiotic Stress Tolerance in Tomato

et al. 2011

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Tomato (Solanum lycopersicum L.) is the second most important vegetable crop in the world after potato (Solanum tuberosum L.), and its productivity is influenced by different abiotic stresses. Though cultivated tomato is moderately tolerant to various abiotic stresses, the crop losses due to unfavorable environmental conditions can be unpredictably severe. So far, several efforts have been made to improve abiotic stress tolerance in cultivated tomato through cultural practices, breeding techniques, and biotechnological approaches. Introgression of abiotic stress tolerance to cultivated tomato from more tolerant wild relatives through classical breeding has been attempted with limited success. However, genetic engineering based on the introgression of genes that are known to be involved in stress response and putative stress tolerance could provide powerful tools for improving abiotic stress tolerance in tomato coupled with the growing knowledge of stress physiology. The present review summarizes the current status and future directions on the use of biotechnological approaches to improve abiotic stress tolerance in tomato.

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Use of phosphomannose isomerase-based selection system for Agrobacterium-mediated transformation of tomato and potato

Cited by 28 publications

References 45 publications

Agrobacterium-mediated genetic transformation of plants: The role of host

Agrobacterium-mediated genetic transformation of plants: The role of host

Efficient production of transgenic tomatoes via Agrobacterium-mediated transformation

An Update on Biotechnological Approaches for Improving Abiotic Stress Tolerance in Tomato

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