Use of Photochemiluminescence for the Determination of Antioxidant Activities of Carotenoids and Antioxidant Capacities of Selected Tomato Products

Abstract

The purpose of this study was to compare the antioxidant activity of carotenoids to that of α-tocopherol by using a photochemiluminescence method (PCL). Comparisons to results obtained by using other established methods (α-TEAC, FRAP, DPPH, LPSC) were carried out. A relationship between the chemical structure of carotenoids and their antioxidant activity could be discussed. The number of conjugated double bonds and functional groups had a positive influence. Additionally, the lipophilic antioxidant capacities of selected tomato products were analyzed using PCL and α-TEAC. HPLC was used to identify and quantify contents of carotenoids, tocopherols, and tocotrienols. The raw material fresh tomatoes had the lowest antioxidant effect in both assays due to the lower carotenoid content. Tomato ketchup had the highest AOC in PCL, and tomato puree, in α-TEAC. The antioxidant capacities were mainly dependent on the water content of the samples. Furthermore, a concentration-dependent effect could be observed in both assays.

Development, properties, and stability of antioxidant shrimp muscle protein films incorporating carotenoid-containing extracts from food by-products

“…Comparing the results of lipophilic antioxidant activity, it is evident that the antioxidant activity of the extracts from tomato and especially from shrimp was higher than that of b-carotene. Bauerfeind et al (2014) found that the antioxidant activity of astaxanthin was approximately 2-fold greater than that of b-carotene and lycopene, also measured by photochemiluminescence. However, the huge difference in antioxidant activity between the lipid extracts and commercial bcarotene found in the present work may have another explanation apart from the different antioxidant activity of carotenoid molecules.…”

“…However, the huge difference in antioxidant activity between the lipid extracts and commercial bcarotene found in the present work may have another explanation apart from the different antioxidant activity of carotenoid molecules. It is very feasible that a considerable fraction of the measured antioxidant activity of the S_E and T_E extracts was attributable to the presence of other antioxidants, for example a-tocopherol, which is also naturally present in these raw materials (Bauerfeind et al, 2014;Sanches-Silva et al, 2011), hydrophobic amino acids, etc.…”

In Vitro Lipophilic Antioxidant Capacity, Antidiabetic and Antibacterial Activity of Citrus Fruits Extracts from Aceh, Indonesia

“…Carotenoid contents of the samples were analysed using a C 30 HPLC column according to a method previously described by [21]. Briefly, analysis was performed on HPLC apparatus with diode array detector at 450 nm (Merck Hitachi, Darmstadt, Germany) using a Develosil RP-aqueous (250 mm × 4.6 mm, 5 µm) C 30 column (Phenomenex, Aschaffenburg, Germany) at 13 ± 1 °C and a gradient of methanol and methyl tert-butyl ether as mobile phase.…”

Peer seeds: Rich source of antioxidants

“…Chemical analysis of carotenoid rich extracts of seeds and oils of two Salvia species controls were carried using reversedphase HPLC with diode array detector at 450 nm according to methods previously described (Bauerfeind et al, 2014). The chromatographic separation was performed on a Develosil® RPAQUEOUS C30 column (250 x 4.6 mm i.d., 5 µm particle size).…”