Use of Pi5(t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea

Abstract

Identification of the PCR markers tightly linked to genes that encode important agronomic traits is useful for marker-assisted selection (MAS). The rice Pi5(t) locus confers broad-spectrum resistance to Magnaporthe grisea, the causal agent of rice blast disease. It has been hypothesized that the Pi5(t) locus carries the same gene as that encoded by the Pi3(t) and Pii(t) loci. We developed three PCR-based dominant markers (JJ80-T3, JJ81-T3, and JJ113-T3) from three previously identified BIBAC clones-JJ80, JJ81, and JJ113-that are linked to the Pi5 (t) locus. PCR analysis of 24 monogenic lines revealed that these markers are present only in lines that carry Pi5 (t), Pi3(t), and Pii(t). PCR and DNA gel-blot analysis of candidate resistance lines using JJ80-T3, JJ81-T3, and JJ113-T3 indicated that Tetep is the likely donor of Pi5(t).Of the 184 rice varieties tested, 34 carried the JJ80-T3-, JJ81-T3-, and JJ113-T3-specific bands. Disease evaluation of those 34 varieties revealed that all conferred resistance to PO6-6. The genomic structure of three of these resistant varieties (i.e., IR72, Taebaeg, Jahyangdo) is most similar to that of Pi5(t). Our results demonstrate the usefulness of the JJ80-T3, JJ81-T3, and JJ113-T3 markers for MAS for M. grisea resistance.

Resistance Genes and Selection DNA Markers for Blast Disease in Rice (Oryza sativa L.)

“…Therefore, one marker set which is useful in a specific cross combination does not always work well in other cross combinations. Although several reports showed that linkage markers can be successfully used to screen for varieties with resistance to rice blast 18,101,107 , almost all markers have never been evaluated as to whether they can be applied to other cross combinations. Information about the markers and their applicability to the combinations of the strains will be valuable for rice breeding using MAS.…”

Differential Requirement of Oryza sativa RAR1 in Immune Receptor-Mediated Resistance of Rice to Magnaporthe oryzae

“…Dongjin and Lijiangxintuanheigu (LTH) and two monogenic resistance lines, IRBLb-B carrying Pib and IRBL5-M carrying Pi5 (Tsunematsu et al, 2000;Yi et al, 2004), were obtained from the National Crop Experiment Station, RDA, Suwon, Korea. Dongjin and LTH were used as the respective susceptible controls to the M. oryzae isolates, PO6-6 and race 007.…”

“…AB013448; Table 1). The sequence characterized amplified region (SCAR) markers JJ80-T3-F/R and JJ113-T3-F/R (Table 1; Yi et al, 2004) were used to determine the presence of Pi5 in genomic DNA. Genotyping of OsRAR1 and PCR amplifications were performed as described previously.…”

“…To examine whether OsRAR1 functions in NB-LRR gene-mediated resistance in rice, we utilized two genes, Pib (Wang et al, 1999) and Pi5 (Lee et al, 2009b), which confer race-specific resistance to M. oryzae and encode NB-LRR proteins. IRBLb-B carrying Pib and IRBL5-M carrying Pi5 (Tsunematsu et al, 2000;Yi et al, 2004) were crossed with osrar1. The resulting F 1 hybrid rice lines each contain a single NB-LRR gene and the mutant osrar1 allele.…”

Development of an Efficient Agrobacterium-Mediated Transformation System and Production of Herbicide-Resistant Transgenic Plants in Garlic (Allium sativum L.)

“…The digested DNA was transferred onto a Hybond N+ nylon membrane (Sambrook et al, 1989), and hybridization was performed using a [α-32 P] dCTP-labeled PAT gene-specific probe prepared using the Rediprime DNA Labeling kit (Amersham Biosciences) in accordance with standard procedures for high-stringency hybridization conditions (Yi et al, 2004). The blot was hybridized in solution containing 0.5 M sodium phosphate (pH 7.2), 1 mM EDTA, 1% (w/v) BSA, and 7% (w/v) SDS for 20 h at 60°C.…”