Use of pineapple waste for single cell protein (SCP) production and the effect of substrate concentration on the yield

Abstract

Single cell protein (SCP) is used as a protein supplement in animal feed and protein rich foods for humans. In this study, extracts of pineapple waste were used as substrate for SCP production using Saccharomyces cerevisiae sp. (Baker or brewing yeast). The study also focused on the effect of the pineapple substrate concentration on the yield of SCP. Pineapple waste were collected from food processing companies in Kumasi and processed into pineapple juice extracts. The residual soluble sugars in the extract were measured and the concentration adjusted (40-100% v/v) for various submerged yeast fermentations to produce the SCPs. Initially, the yeast cells were cultured on Yeast Potato Dextrose Agar (YPDA) and then transferred into a Nutrient Broth to generate the activated yeast inoculum for the final fermentation. Pineapple extracts in 2.50 3 10 24 m 3 conical flasks at various concentrations were each inoculated with 1 mL inoculum. The set-ups were incubated at 298 K and the yeast cells or colonies counted at 24 hr interval for 5 days. Biochemical analysis of the substrate revealed the following composition: pH of 5.5 and 4.7% (m/v) total soluble sugars. Maximum yield and doubling time/hr of SCPs (3.01 kg/m 3 , 1,108 hr) was observed for the 60% (v/v) pineapple substrate concentration. The 100% (v/v) substrate concentration pro-duced the least SCP yield of 2.5 3 10 22 kg/m 3 . It is concluded from this study that pineapple waste generated in the food processing industry serves as good substrates for SCP production with high-potential economic value. Practical applicationsSingle cell proteins are utilized mainly as food supplement for human beings and animals. Several other applications of single cell proteins are available. It is used as a health food to control obesity, and provide instant energy to especially sports men. It is also used in therapeutic and natural medicines to: reduce body weight, cholesterol, stress, lower blood sugar in diabetic patients, promote healthy eyes and skin, and increase lactation. It is further used in the technical field for paper and leather processing, and as foam stabilizer. K E Y W O R D S growth rate, pineapple waste, Saccharomyces cerevisiae, single cell proteins (SCP)

Formulation of a fermentation substrate from pineapple and sacha inchi wastes to grow Weissella cibaria

“…The protein content of FPP and FPC was low, while SI had a considerably high protein and lignosellulosic content (Table 2). Therefore, SIS favours SFS since N 2 is a necessary element in microbial metabolism during cell growth (Mensah and Twumasi, 2017). Similar protein contents in SIS (59%) have been reported by Wang et al (2018).…”

“…Among the evaluated residues, the pineapple peel is outstanding due to the high concentration of reducing sugars (97 g L À1 ) obtained through the application of acid hydrolysis as a pre-treatment (Vega-Castro et al 2016). In addition, pineapple waste has been used to produce cellular protein (Mensah and Twumasi, 2017). Pineapple is part of the bromeliad family, and, in 2017, world production was 27 million tons with a harvested area of 1 million ha.…”

Isolation and Characterization of Ralstonia solanacearum Causing Bacterial Wilt of Solanaceae Crops

“…In all isolates the colony forming units were uncountable at zero dilution. This result in conformity with Adams et al, (2014), Kwiatkowski et al, (2015), Mensah and Twumasi (2016), Hendry et al, (2014), even they could find 40 plates out of 1440 were having uncountable colonies due to spreading of the colonies. It was observed that the plates were having uncountable colonies at zero dilution because of more number of colonies and due to spreading.…”

An overview of applications in pineapple agroindustrial residues

“…The use of agroindustrial residues of pineapple as a supplement usually involves the use of microorganisms for protein enrichment. For example, Mensah and Twumasi (2017) used pineapple residues as a substrate for production of Single Cell Protein (SCP) with yeast Saccharomyces cerevisiae as inoculum. According to its results, it was concluded that residues from the food processing industry serve as good substrates for SCP production, which presents high benefit.…”