Traditional systems of medicine have become a topic of global importance recently. Increased commercialization of economically important medicinal plants has resulted in overharvesting and threatening their survival. The present study was carried out to document the indigenous uses of medicinal plants by the local communities in and around Rema-Kalenga Wildlife Sanctuary, Bangladesh. Data collection was predominantly qualitative recording the species use, identifying their relative importance (RI) and assessing the informants' consensus factor (F ic ) on associated knowledge. We interviewed 140 households of the local community and 5 local herbal practitioners. A total of 44 plant species were in use against 33 ailments under 10 broad disease categories. Five species were found to have high use versatility (RI>1), Emblica officinale L. being the most versatile. Respiratory problems scored the highest F ic value (0.56) involving the use of 30% species recorded. Terminalia bellerica Roxb., Sterculia villosa Roxb., Dillenia pentagyna Roxb. and Terminalia arjuna Bedd. were being harvested commercially. Use by the community, particularly for subsistence consumption ensured sustainable harvesting, but commercial extraction of some species appeared unsustainable. Buffer zone-based commercial farming of medicinal plants with a commercial value could serve a dual purpose of assuring sustainable Alternative Income Generation (AIG) for local communities and conserving the natural resources in protected areas.