Use of plastic tips in artificial feeding of Dermacentor (Anocentor) nitens females Neumann, 1897 (Acari: Ixodidae)

Ribeiro, Carla Carolina Dias Uzedo; Baêta, Bruna de Azevedo; Valim, Jaqueline Rodrigues de Almeida; Teixeira, Rafaella Câmara; Cepeda, Patrícia Barizon; Silva, Jenevaldo Barbosa da; Fonseca, Adivaldo Henrique da

doi:10.1016/j.ttbdis.2014.05.012

Cited by 8 publications

(5 citation statements)

References 7 publications

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“…Artificial feeding with the new device did not interfere with the biology of the ticks, as similarly observed in the species D. nitens by Ribeiro et al (2014).…”

Section: Discussionsupporting

confidence: 66%

“…The feeding system used 1000 μl plastic tips and followed the methods described by Ribeiro et al (2014). The donor blood used in the feed was directly collected from the cephalic vein of a healthy dog through a vacuum system into a tube containing the anticoagulant sodium citrate.…”

Section: Methodsmentioning

confidence: 99%

“…Capillary tubes have proved to be very effective for this group, showing promise for studies on pathogen isolation Plastic tips in artificial feeding of R. sanguineus (RANGEL et al, 2008). Plastic tips have been used as a new alternative to automated artificial feeding techniques for ticks and were first described by Ribeiro et al (2014) for feeding Dermacentor nitens.…”

Section: Introductionmentioning

confidence: 99%

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Using plastic tips in artificial feeding of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) females

Valim

Rangel

Baêta

et al. 2017

Rev. Bras. Parasitol. Vet.

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This study evaluated the influence of the initial weight, feeding period and temperature on weight gain and biological parameters of the non-parasitic phase of partially engorged Rhipicephalus sanguineus females that were artificially fed using plastic tips as feeding devices. The device did not alter the oviposition of the females or any other parameters evaluated. Furthermore, it was observed that the temperature of the feeding the group did not affect the weight gain and biology of ticks. This device has great potential for the development of studies on bioagent transmission because it provides higher intake of blood by ixodid ticks.Keywords: In vitro feeding, plastic tips, biological parameters, Rhipicephalus sanguineus. ResumoEste estudo avaliou a influência do peso inicial, período de alimentação e temperatura no ganho de peso e parâmetros biológicos da fase não parasitária, de fêmeas parcialmente ingurgitadas de Rhipicephalus sanguineus alimentadas artificialmente utilizando ponteiras plásticas como dispositivo de alimentação. O dispositivo não alterou a oviposição das fêmeas ou quaisquer outros parâmetros avaliados. Além disso, observou-se que a temperatura de alimentação do grupo não afetou o ganho de peso e a biologia dos carrapatos. Este dispositivo tem um grande potencial para o desenvolvimento de estudos sobre a transmissão de bioagentes, uma vez que proporciona maior ingestão de sangue por carrapatos ixodídeos.Palavras-chave: Alimentação in vitro, ponteiras plásticas, parâmetros biológicos, Rhipicephalus sanguineus.

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“…Artificial feeding with the new device did not interfere with the biology of the ticks, as similarly observed in the species D. nitens by Ribeiro et al (2014).…”

Section: Discussionsupporting

confidence: 66%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Using plastic tips in artificial feeding of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) females

Valim

Rangel

Baêta

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…Nearly-engorged adult female A . sculptum were detached from rabbits and weighed before being artificially fed, using plastic pipette tips [40,41] as follows. The ticks were divided into two groups; one group received at least 400μL of citrated rabbit blood containing 10 7 /mL viable M .…”

Section: Methodsmentioning

confidence: 99%

Ticks as potential vectors of Mycobacterium leprae: Use of tick cell lines to culture the bacilli and generate transgenic strains

Ferreira¹,

Oliveira²,

Santos³

et al. 2018

PLoS Negl Trop Dis

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Leprosy is an infectious disease caused by Mycobacterium leprae and frequently resulting in irreversible deformities and disabilities. Ticks play an important role in infectious disease transmission due to their low host specificity, worldwide distribution, and the biological ability to support transovarial transmission of a wide spectrum of pathogens, including viruses, bacteria and protozoa. To investigate a possible role for ticks as vectors of leprosy, we assessed transovarial transmission of M. leprae in artificially-fed adult female Amblyomma sculptum ticks, and infection and growth of M. leprae in tick cell lines. Our results revealed M. leprae RNA and antigens persisting in the midgut and present in the ovaries of adult female A. sculptum at least 2 days after oral infection, and present in their progeny (eggs and larvae), which demonstrates the occurrence of transovarial transmission of this pathogen. Infected tick larvae were able to inoculate viable bacilli during blood-feeding on a rabbit. Moreover, following inoculation with M. leprae, the Ixodes scapularis embryo-derived tick cell line IDE8 supported a detectable increase in the number of bacilli for at least 20 days, presenting a doubling time of approximately 12 days. As far as we know, this is the first in vitro cellular system able to promote growth of M. leprae. Finally, we successfully transformed a clinical M. leprae isolate by inserting the reporter plasmid pCHERRY3; transformed bacteria infected and grew in IDE8 cells over a 2-month period. Taken together, our data not only support the hypothesis that ticks may have the potential to act as a reservoir and/or vector of leprosy, but also suggest the feasibility of technological development of tick cell lines as a tool for large-scale production of M. leprae bacteria, as well as describing for the first time a method for their transformation.

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“…The calf used was not under antibiotic or acaricidal therapy for 2 months before the experiment. Artificial feeding of R. microplus was adapted from Valim et al [ 31 ] and Ribeiro et al [ 32 ]. Partially engorged tick females were weighed, surface sterilized with sodium hypochlorite (0.05% v/v) for 3 min and dried with paper towels.…”

Section: Methodsmentioning

confidence: 99%

Entomopathogenic fungus treatment changes the gut bacterial diversity of Rhipicephalus microplus ticks

et al. 2023

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Background Ticks are obligate bloodsucking parasites responsible for significant economic losses and concerns with human and animal health, mainly due to the transmission of pathogens. Entomopathogenic fungi have been intensively studied as an alternative strategy for tick control that can be used in combination with synthetic acaricides in the integrated management of ticks. Here, we investigated how the gut bacterial community of Rhipicephalus microplus is shaped after Metarhizium anisopliae treatment and how the tick susceptibility to the fungus is affected after disrupting gut bacterial microbiota. Methods Partially engorged tick females were artificially fed with pure bovine blood or blood plus tetracycline. Two other groups received the same diet and were topically treated with M. anisopliae. The guts were dissected, and the genomic DNA was extracted 3 days after the treatment; the V3–V4 variable region of the bacterial 16S rRNA gene was amplified. Results The gut of ticks that received no antibiotic but were treated with M. anisopliae exhibited lower bacterial diversity and a higher occurrence of Coxiella species. The Simpson diversity index and Pielou equability coefficient were higher in the gut bacterial community when R. microplus were fed with tetracycline and fungus-treated. Ticks from fungus-treated groups (with or without tetracycline) exhibited lower survival than untreated females. Previous feeding of ticks with the antibiotic did not change their susceptibility to the fungus. Ehrlichia spp. were not detected in the gueated groups. Conclusions These findings suggest that myco-acaricidal action would not be impacted if the calf hosting these ticks is under antibiotic therapy. Moreover, the hypothesis that entomopathogenic fungi can affect the bacterial community in the gut of R. microplus engorged females is endorsed by the fact that ticks exposed to M. anisopliae exhibited a dramatic reduction in bacterial diversity. This is the first report of an entomopathogenic fungus affecting the tick gut microbiota. Graphical Abstract

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Use of plastic tips in artificial feeding of Dermacentor (Anocentor) nitens females Neumann, 1897 (Acari: Ixodidae)

Cited by 8 publications

References 7 publications

Using plastic tips in artificial feeding of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) females

Using plastic tips in artificial feeding of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) females

Ticks as potential vectors of Mycobacterium leprae: Use of tick cell lines to culture the bacilli and generate transgenic strains

Entomopathogenic fungus treatment changes the gut bacterial diversity of Rhipicephalus microplus ticks

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