Use of Pleurotus dryinus for lignocellulolytic enzymes production in submerged fermentation of mandarin peels and tree leaves

Elisashvili, Vladimir; Penninckx, Michel; Kachlishvili, Eva; Asatiani, Mikheil D.; Kvesitadze, G.

doi:10.1016/j.enzmictec.2005.08.033

Cited by 94 publications

(56 citation statements)

References 23 publications

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“…The laccase production varied significantly between Ammonium sulphate and all other nitrogen sources (Graph 8). 72 have shown that medium with ammonium sulphate has given highest levels of laccase activity in Cerrena unicolor. Zahida et al, 68 have reported that the greatest production of laccase, chick pea powder was used as nitrogen source in solid state fermentation.…”

Section: Influence Of Nitrogen Sources For Laccase Productionmentioning

confidence: 99%

“…Thus, determination of optimum inoculum size becomes a crucial step in SSF. In previous studies different inoculums size have been used for laccase production, four Pleurotuspulmonarius mycelial plugs measuring 10 mm in diameter were used for inoculation of 5 g of wheat bran 10 . Moleds et al, 64 have observed that the highest specific activity for laccase was obtained when 20 Trametes hirsute agar plugs measuring 3 mm in diameter for inoculation 2.5 gm barley bran or 5 g grape seed was used.…”

Section: Graph 5: Effect Of Inoculum Size On Laccase Production By Mamentioning

confidence: 99%

“…approaches for this purpose is to utilize the potential of lignocellulosic wastes, some of which may contain significant concentrations of soluble carbohydrates and inducers of enzyme synthesis ensuring efficient production of ligninolytic enzymes 10,11 . SSF is defined as any fermentation process performed on a non-soluble material that acts both as physical support and source of nutrients in absence of free flowing liquid 12 .…”

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confidence: 99%

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2016

IJPSR

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ABSTRACT:The aim of the present study was to investigate the production of laccase by Marasmius sp. BBKAV79 in Solid State Fermentation (SSF) and its antiproliferative activity. Experiments were also conducted to determine the variables that could affect activity of laccase. In particular, the effects of the initial moisture content, pH, temperature, and inoculum volume, incubation period in addition to carbon and nitrogen sources were evaluated. Rice bran was found to be the best supported lignocellulosic substrate for extracellular laccase production under SSF. Highest activity of laccase was achieved at pH 6.0 and temperature at 40°C. Inoculum size and incubation period for laccase production at 2000µl culture broth and 10 days respectively. The presence of starch and ammonium sulphate in the growth medium was found to be favourable for the production of the laccase. Such a high activity was obtained without any addition of inducers. Thus, the indigenous isolate is found to be a potential producer of laccase using SSF and can be exploited for further biotechnological applications. The process also promises economical utilization and value addition of agroresidues and it potently suppressed the proliferation of tumor cell lines KB, MCF7 and VERO with an IC50 value of 88.61 µl, 49.5 µl, and ~67603 µl respectively, signifying that it is an anticancer protein.

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Section: Influence Of Nitrogen Sources For Laccase Productionmentioning

confidence: 99%

Section: Graph 5: Effect Of Inoculum Size On Laccase Production By Mamentioning

confidence: 99%

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confidence: 99%

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2016

IJPSR

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“…Various lignocellulosic substrates and whiterot fungi have been used successfully in submerged and solid-state fermentation for lignocellulolytic enzyme production. The type and composition of lignocellulosic substrate appear to determine the type and amount of enzyme produced by basidiomycetes (Elisashvili et al, 2006). Compared with submerged fermentation, solidstate fermentation provides certain advantages of fungal enzyme production with the aspect of application in bioprocesses such as biobleaching, biopulping, bioremediation etc (Stajic et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Cultivation of Oyster Mushroom and Recovery of Value Added by- products from Biodegradable Lignocellulosic Waste Materials by Solid State Fermentation

Padhye¹,

Atawane²

2017

Int.J.Curr.Res.Aca.Rev.

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Article InfoIn the present study, the edible fungus Pleurotus sajor-caju was investigated for its ability to grow on different lignocellulosic substrates namely, water hyacinth, wood chips and paddy and to produce various lignocellulolytic enzymes such as cellulase, xylanase and pectinase. The production pattern of the extracellular enzymes, total proteins and reducing sugar level was studied during the growth of this fungus under Solid State Fermentation (SSF) for a period of 56 days. Wheat grains were rapidly utilized and consumed by the fungus as a simple carbon source and were used for spawn formation. Different lignocellulosic complex carbon sources served as substrates and exhibited better induction process for more enzyme biosynthesis and production. The maximum activity of enzymes was obtained on 28 th and 56 th day of culture growth. The total yield of mushroom grown on different lignocellulosic substrates and levels of cellulose, pectinase and xylanase was also detected.

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“…For example, Baldrian et al (2005) reported the production of cellulolytic and hemicellulolytic enzymes endo-1,4-β-glucanase, exo-1,4-β-glucanase, 1,4-β-glucosidase, endo-1,4-β-xylanase, 1,4-β-xylosidase, endo-1,4-β-mannanase and 1,4-β-mannosidase, and ligninolytic enzymes such as Mn-peroxidase and laccase, by growing P. ostreatus on wheat straw in the presence and absence of Cu, Mn, Pb, and Zn. Elisashvili et al (2006) studied the production of cellulase, xylanase, laccase and manganese peroxidase in submerged fermentation of mandarin peels and tree leaves. Thakur et al (2013) reported the production of ligninolytic enzymes using P. ostreatus from biologically and chemically pretreated wheat straw and banana stem substrates.…”

Section: Introductionmentioning

confidence: 99%

Production of lignocellulolytic enzymes from floriculture residues using Pleurotus ostreatus

et al. 2014

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Floriculture is a vital agro-industrial sector in the Colombian economy; the export of flowers positively impacts employment and the balance of trade. However, this industry could negatively impact the environment if its waste products are not handled properly. These flower residues, rich in lignin, hemicellulose and cellulose, could be a cost-effective raw material to produce enzymes. Here, we evaluate the production of lignocellulolytic enzymes by degradation of Chrysanthemum and Rosa residues using Pleurotus ostreatus, and manganese sulfate and copper sulfate as inductors. From the two residues, we obtained laccase, manganese peroxidase, endoglucanase, exoglucanase, and β-glucosidase. The use of inductors, favored all enzyme activities except for β-glucosidase. The enzymes that displayed the highest activity were laccase (4,693.4 U/L and 2,640 U/L from the residues of Chrysanthemum and Rosa, respectively) and β-glucosidase (9,513 U/L and 6,811.9 U/L). The enzyme that showed the lowest activity was endoglucanase (11.5 U/L and 15.4 U/L). Under the conditions evaluated, the best substrate for enzyme production is Chrysanthemum wastes; the extracts obtained had higher enzymatic activity than the extracts from Rosa residues.

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Use of Pleurotus dryinus for lignocellulolytic enzymes production in submerged fermentation of mandarin peels and tree leaves

Cited by 94 publications

References 23 publications

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Cultivation of Oyster Mushroom and Recovery of Value Added by- products from Biodegradable Lignocellulosic Waste Materials by Solid State Fermentation

Production of lignocellulolytic enzymes from floriculture residues using Pleurotus ostreatus

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