Flocculation is extensively employed for clarification through sedimentation. Application of ecofriendly plant-based bio-flocculants in wastewater treatment has attracted significant attention lately with high removal capability in terms of solids, turbidity, colour and dye. However, moderate flocculating property and short shelf life restrict their development. In order to enhance the flocculating ability, natural polysaccharides derived from plants are chemically modified by inclusion of synthetic, non-biodegradable monomers (e.g. acrylamide) onto their backbone to produce grafted bio-flocculants. This review is aimed to provide an overview of the development and flocculating efficiencies of plant-based bio-flocculants and grafted bio-flocculants for the first time. Furthermore, the processing methods, flocculation mechanism and the current challenges are discussed. All the reported studies about plant-derived bio-flocculants are conducted under lab-scale conditions in wastewater treatment. Hence, the possibility to apply natural bio-flocculants in food and beverage, mineral, paper and pulp, oleo-chemical and biodiesel industries is discussed and evaluated.