Use of polymeric membranes for purification of an E. coli expressed biotherapeutic protein

Abstract

Polymers have had a significant impact on the field of bioseparations in the past few decades. Most recently, membrane chromatography has emerged as an efficient alternative to the conventional packed-bed chromatography by eliminating the diffusion-related limitations associated with the traditional resin beads. In this article, we examine six membrane adsorbers for purification of granulocyte colony-stimulating factor (GCSF), an Escherichia coli-based biotherapeutic. These adsorbers differ either in their base matrix or in the surface chemistry. The role of interactions between the filter surfaces and the protein molecules in effecting these separations is the focus of the article.

ATF for cell culture harvest clarification: mechanistic modelling and comparison with TFF

“…Tangential flow filtration (TFF) is the most common form of membrane separation that is used for this purpose . However, TFF performance is marred by membrane fouling and hence a deeper understanding of what causes membrane fouling and how it can be minimized has been a topic of interest to researchers in academia and industry alike …”

“…5, 6 However, TFF performance is marred by membrane fouling and hence a deeper understanding of what causes membrane fouling and how it can be minimized has been a topic of interest to researchers in academia and industry alike. 7,8 The clarification process can be understood as a separation of micrometer sized suspended cells. In general, such separation can deal with mechanisms such as complete blocking of pore mouths, intermediate blocking (partial blocking of pore mouth), standard blocking (pore constriction due to the deposition of particles on pore walls), cake filtration (deposition of particles on membrane surface), inertial migration (tubular-pinch effect), shear-induced hydrodynamic convection, shear-induced hydrodynamic diffusion, 9 as well as eddy diffusion.…”