Use of potentiometric detection in (ultra) high performance liquid chromatography and modelling with adsorption/desorption binding kinetics

Daems, Devin; Camp, Guy Van; Fernández, M.; Guisez, Yves; Prinsen, Els; Nagels, L.J.

doi:10.1016/j.aca.2013.03.031

Cited by 8 publications

(8 citation statements)

References 23 publications

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“…10A). Moreover, the potentiometric ISEs can be used to trace the adsorption/ desorption binding kinetics and small molecule/biomolecule binding kinetics [92,143]. These techniques give new insights into the functioning of ISEs and allow a better understanding of potentiometric ISEs for their use as biosensors.…”

Section: Other Applicationsmentioning

confidence: 99%

Recent advances in potentiometric biosensors

Ding

Qin

2020

TrAC Trends in Analytical Chemistry

229

121

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Potentiometry based on ion-selective electrodes (ISEs) has been injected with new vigor and gone through a renaissance with the improvements in the detection limits and selectivities of ISEs, the introduction of new materials, new sensing concepts (from conventional potentiometry to dynamic electrochemistry approaches), and deeper theoretical understanding and modelling of the potentiometric responses of ISEs. The new breakthroughs encourage innovations in ion sensing and biosensing applications. Moreover, with the introduction of new bioreceptors, such as enzymes, antibodies, aptamers, peptides, versatile sensing protocols have been designed for a broad range of different target molecules by using ISEs as powerful transducers. This paper reviews the recent trends in potentiometric biosensors. Their applications in biosensing of metal ions, small molecules, DNA, proteins, bacteria and toxicities have been discussed. This review provides the outlook of the potentiometric biosensing based on the integration of potentiometric ISEs with new materials and emerging techniques.

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Section: Other Applicationsmentioning

confidence: 99%

Recent advances in potentiometric biosensors

Ding

Qin

2020

TrAC Trends in Analytical Chemistry

229

121

View full text Add to dashboard Cite

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“…The higher effective plate number obtained for CTC and DXC presented better separation efficiency, which is ascribed to the higher content of ACN during their elution. However, a slightly smaller value was obtained for all target TCs with the HPLC-ISE methodology, which may be attributed to the adsorption/desorption phenomena of the analytes at the membrane surface of the TC-selective electrode [22,60]. This fact also explains the differences obtained in the peak symmetry, demonstrated by the tailing factors of 1.4 to 1.6 and 1.1 to 1.2 for potentiometric and UV detectors, respectively.…”

Section: Methods Validationmentioning

confidence: 82%

“…The analytical parameters of the proposed TC-selective electrodes under flow conditions are slightly different than those obtained in static measurements (Table S1, ISM B). Under flow conditions, the residence and response time, the rate of diffusion and the exchange reaction of the eluent ions, the sample dispersion and dilution, namely in flow-cell, affects the analytical performance of the proposed potentiometric detector, as has already been reported by other authors [22,48,[53][54][55]. To overtake these constraints, the use of carbon nanotubes in the sensing membrane was considered.…”

Section: Optimization Of Hydrodynamic Conditionsmentioning

confidence: 96%

“…The coupling of potentiometric detection with liquid chromatography was considered decades ago by using metallic copper electrodes [16][17][18] for the determination of inorganic ions in ion chromatography systems. Later on, Luc Nagels and his group extended the applications to organic ionic substances, including organic acids [19], basic drugs [20,21], and alkaloids [22], resorting to polymeric membrane electrodes based on a conductive support. Despite their powerful features, potentiometric sensors are still not viewed as routine detectors in HPLC and thus researchers should put more effort into demonstrating their competitiveness with other detectors.…”

Section: Introductionmentioning

confidence: 99%

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Cucurbit[8]uril-Based Potentiometric Sensor Coupled to HPLC for Determination of Tetracycline Residues in Milk Samples

et al. 2022

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The determination of chlortetracycline, doxycycline, oxytetracycline, and tetracycline in milk samples by HPLC coupled to a cucurbit[8]uril-based potentiometric sensor is herein presented. The new tetracycline-selective electrode is based on a polymeric membrane incorporating cucurbit[8]uril as a macrocyclic host, potassium tetrakis(p-chlorophenyl) borate as an ionic additive, 2-fluorophenyl 2-nitrophenyl ether as a plasticizer, and multi-walled carbon nanotubes as nanostructured materials. A microfluidic wall-jet flow-cell is implemented as a potentiometric detector after chromatographic separation by a C8 column using a gradient mobile phase of sulphuric acid and acetonitrile. The proposed methodology was validated following International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) and European Union (EU) guidelines. Linear regression models provided R2 in the range from 0.9973 ± 0.0026 to 0.9987 ± 0.0012 for all tetracycline antibiotics. The limits of detection and quantification ranged from 13.3 to 46.0 μg L−1 and 44.4 to 92.1 μg L−1, respectively. Precision intra-day, inter-day, and inter-electrode showed relative standard deviation values lower than 12.5%, 13.5%, and 12.9%, respectively. Accuracy was assessed by analysis of spiked milk samples around the maximum residue limit, yielding recovery values in the range from 81.3 to 108.5%. The simple, sensitive, cost-effective, and reliable HPLC-ion-selective electrode method justifies its use as a competitive alternative for the analysis of tetracycline residues in the food quality control sector.

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“…Detectors with low cell volume based on conductivity [11][12][13], potentiometry [14,15], amperometry (AD) [1][2][3][4][5][6][7][8][9][10], or pulsed amperometry (PAD) [16,17] can be located online in close proximity, in a post-column configuration, to a liquid chromatographic setup or inserted as microelectrodes close to a capillary end [18,19]. A miniaturized electrolysis cell can also be part of an online setup such as in a FIA system or of a pre-or post-LC column coupled to a mass spectrometer for electrochemical product generation and characterization [20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%