2003
DOI: 10.17221/3720-cjgpb
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Use of Prolamin Polymorphism to Describe Genetic Variation in a Collection of Barley Genetic Resources

Abstract: Abstract:The polymorphism of prolamin storage proteins was studied in seed samples of 20 historical cultivars of spring barley (Hordeum vulgare L.) of Czech and Slovak origin, using polyacrylamide gel electrophoresis (PAGE). Only two samples were uniform. Most heterogeneity of prolamin patterns was observed in the oldest accessions. By means of a prolamin identity index it was possible to distinguish sister lines from admixtures within the seed samples. The obtained spectra will be used as additional descripto… Show more

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Cited by 4 publications
(6 citation statements)
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“…Electrophoretic analysis is most commonly applied for hordein separation; it enables to study their allelic variability very effectively. Therefore, it is very suitable for the differentiation and identifi cation of the individual genotypes of barley (Vyhnánek et al, 2003;Bleidere and Grunte, 2009). The biochemical techniques most widely employed for separation of proteins or study of the hordein genetic structure include electrophoretic methods in polacrylamide gel (SDS PAGE, A PAGE) (Yin et al, 2003;Baloch et al, 2014) and starch gel (Šašek et al, 1990;Pomortsev et al, 2001;2012;Psota and Bradová, 2009;Lyalina et al, 2016).…”
Section: Frekvence Hordeinových Alelmentioning
confidence: 99%
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“…Electrophoretic analysis is most commonly applied for hordein separation; it enables to study their allelic variability very effectively. Therefore, it is very suitable for the differentiation and identifi cation of the individual genotypes of barley (Vyhnánek et al, 2003;Bleidere and Grunte, 2009). The biochemical techniques most widely employed for separation of proteins or study of the hordein genetic structure include electrophoretic methods in polacrylamide gel (SDS PAGE, A PAGE) (Yin et al, 2003;Baloch et al, 2014) and starch gel (Šašek et al, 1990;Pomortsev et al, 2001;2012;Psota and Bradová, 2009;Lyalina et al, 2016).…”
Section: Frekvence Hordeinových Alelmentioning
confidence: 99%
“…Heterogenní odrůdy jsou charakterizované vyšším počtem hordeinových linií (biotypů), (Dimova et al, 2010). Řada autorů popisuje vnitroodrůdový polymorfi smus, který je důležitý pro charakterizaci odrůd ječmene (Radovic a Vapa, 1996; Echart-Almeida a Cavalli-Molina, 2000; Vyhnánek et al, 2003). Jeho znalost umožňuje stanovit genetickou strukturu heterogenních odrůd, která je dána počtem hordeinových linií, případ-ně jejich procentním zastoupením v odrůdě (Bradová et al, 2001;Psota a Bradová, 2009).…”
Section: Vnitroodrůdový Polymorfi Smusunclassified
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