Use of Proline to Induce Salt Stress Tolerance in Guava

Abstract

Guava is a fruit tree with high potential in the semi-arid region of northeast Brazil. However, qualitative and quantitative water scarcity is a limiting factor for the expansion of irrigated agriculture. Thus, it is necessary to use techniques to mitigate the effects of salt stress, such as foliar application of proline. The objective of this study was to evaluate the effect of foliar application of proline as a mitigator of salt stress effects on the morphophysiology of guava cv. Paluma. The experiment was carried out under field conditions at the ‘Rolando Enrique Rivas Castellón’ Experimental Farm in São Domingos, PB, Brazil, using a randomized block design in a 5 × 4 factorial scheme referring to five levels of electrical conductivity of irrigation water, ECw (0.8, 1.5, 2.2, 2.9, and 3.5 dS m−1) and four concentrations of proline (0, 8, 16, and 24 mM). Salinity above 0.8 dS m−1 compromised gas exchange, photosynthetic pigment synthesis, photochemical efficiency, and growth of guava plants at 360 days after transplanting. Foliar application of proline at a concentration of 24 mM mitigated the effect of salt stress on the relative water content, stomatal conductance, and carotenoid contents in plants irrigated with 3.6 dS m−1 water. Meanwhile, a proline concentration of up to 18 mM resulted in higher transpiration, CO2 assimilation rate, instantaneous carboxylation efficiency, and absolute growth rate in stem diameter under ECw of 0.8 dS m−1. Proline concentration of up to 24 mM increased the biosynthesis of photosynthetic pigments and the relative growth rate in stem diameter of guava in the period from 190 to 360 days after transplanting.