Use of ProteinChip™ array surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) to identify thymosin <i>β</i>-4, a differentially secreted protein from lymphoblastoid cell lines

Diamond, Deborah L.; Zhang, Yanni; Gaiger, Alexander; Smithgall, Molly D.; Vedvick, Thomas S.; Carter, Darrick

doi:10.1016/s1044-0305(03)00265-4

Cited by 31 publications

(18 citation statements)

References 15 publications

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“…Increased levels of human thymosin beta4 were found in melanoma-derived metastases (Clark 2000, Ridley 2000 and in a malignant mouse fibrosarcoma cell line (Kobayashi 2002). Thymosin beta4 was also detected in Epstein-Barr virus transformed B-cells in a differential screening experiment carried out by Diamond et al (2003). Wang et al (2003) could demonstrate changes in cultivated colon cancer cells towards malignant progression after overexpressing thymosin beta4.…”

Section: Introductionmentioning

confidence: 94%

Expression of thymosin beta4 during chick development

Dathe

Brand‐Saberi

2004

Anatomy and Embryology

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We cloned the chick homologue of Homo sapiens thymosin beta4, encoding a G-actin sequestering factor which plays an important role in angiogenesis, cell motility and tumorigenesis. The thymosin beta4 gene is highly conserved between chick and human. Its expression was analyzed during different stages of development. At early stages thymosin beta4 is expressed in the mesoderm and endoderm and in Hensen's node. Later, thymosin beta4 transcripts are found in the head mesenchyme, somites, dorsal root ganglia, neural tube, brain, blood vessels and feather buds. The pattern of thymosin beta4 expression in blood vessels indicates a function mainly in development of the blood circulatory system which closely parallels findings in vitro. The observed expression pattern shows a high similarity to expression data published for mice, mainly in the heart and in the nervous system. Important new aspects are the early onset of expression, the expression in the mesoderm preceding heart formation and the involvement in feather development.

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Section: Introductionmentioning

confidence: 94%

Expression of thymosin beta4 during chick development

Dathe

Brand‐Saberi

2004

Anatomy and Embryology

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“…Since the first report of Tb10 upregulation in renal cell carcinomas in 1991 [24], DNA array methodology has revealed a frequent correlation between dysregulated b-thymosin expression and tumour progression [9,29,[43][44][45][46][47][48][49][50][51][52]. In particular, increased b-thymosin levels were associated with augmented metastatic potential, presumably reflecting the need of these cell types both to migrate [43,44,47] and induce vascularisation.…”

Section: Tb4 and Tumour Progressionmentioning

confidence: 99%

Thymosin β4 and angiogenesis: modes of action and therapeutic potential

Smart¹,

Rossdeutsch²,

Riley³

2007

Angiogenesis

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“…However, YB-1, several isoforms of thymosin ␤, and also 14-3-3 have been demonstrated to be upregulated in several kinds of human cancers (17,24,49,51), suggesting that these genes are involved in the progression rather than the induction of tumors.…”

Section: Discussionmentioning

confidence: 99%

Gene Profiling of Cottontail Rabbit Papillomavirus-Induced Carcinomas Identifies Upregulated Genes Directly Involved in Stroma Invasion as Shown by Small Interfering RNA-Mediated Gene Silencing

Huber

Vlasny

Jeckel

et al. 2004

J Virol

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While high-risk human papillomaviruses (HPVs) have been identified as necessary risk factors for the development of cervical cancer (7), the role of HPV in skin cancer, which is the most common cancer in Caucasians, is still undefined (3, 23). A first link between papillomaviruses and skin cancer was obtained in 1935 from an animal model system, when Rous and Beard (40) described the development of squamous cell carcinomas (SCCs) in rabbits after experimental infection with cottontail rabbit papillomavirus (CRPV). Initial benign warts develop into invasively growing SCCs without any further cocarcinogens within 6 to12 months in the vast majority of the animals (50). A similar situation has been described for humans, where in the rare genetic disorder epidermodysplasia verruciformis, patients develop flat HPV-induced warts which progress in up to 60% of the cases into mostly primary SCCs (36, 37). Skin cancer has also been recognized as a common side effect in long-term-immunosuppressed patients, who have a 65-fold-increased risk for this disease. Shortly after the onset of immunosuppressive therapy, HPV-induced warts develop, and after 20 years of immunosuppression, nonmelanocytic skin cancer can be found in up to 70% of the patients (8). As in the case of epidermodysplasia verruciformis patients, the lesions seem to progress from warts through dysplastic lesions into SCC (4,16,43). Although HPV-associated SCCs have been found in 65% (43) or 81% (4) of the cases, the mechanisms leading to the development of skin cancer and especially the role of papillomaviruses are not yet fully understood.Thus far, the only model system for studying the progression of papillomavirus-induced skin tumors is the domestic rabbit infected with CRPV. Infection of domestic rabbits with CRPV particles or viral DNA leads to the development of local tumors within 3 to 6 weeks postinfection. These papillomas progress within 6 to 12 months in more than 80% of the cases into invasively growing and finally metastasizing carcinomas (45; S. Jeckel, unpublished data). Only in very few individual rabbits do papillomas persist in the benign state, and even fewer regress. This progressive behavior does not depend on any other known cofactor besides the viral infection, which makes CRPV an important model for papillomavirus-associated skin disorders in order to study the role of viral and cellular gene expression during cancer development.Earlier studies using more descriptive methods, such as RNA-RNA in situ hybridization, showed no pronounced qualitative or quantitative changes in viral gene expression during malignant progression (50). The primary target cells localized in the bulge region of hair follicles already revealed rather high expression of E6 and E7 mRNAs (42), which remains a constant feature from papillomas through carcinomas to lung metastases (50, 52). Therefore it seems most likely that alterations

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Use of ProteinChip™ array surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) to identify thymosin β-4, a differentially secreted protein from lymphoblastoid cell lines

Cited by 31 publications

References 15 publications

Expression of thymosin beta4 during chick development

Expression of thymosin beta4 during chick development

Thymosin β4 and angiogenesis: modes of action and therapeutic potential

Gene Profiling of Cottontail Rabbit Papillomavirus-Induced Carcinomas Identifies Upregulated Genes Directly Involved in Stroma Invasion as Shown by Small Interfering RNA-Mediated Gene Silencing

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