“…in vivo masticated bolus was used in some studies (Johansson, Gutiérrez, Landberg, Alminger, & Langton, 2018), but more than often, simple mechanical disruptive methods were used to simulate size reduction of chewing. Chopping/ cutting (Ficco et al, 2016;Woolnough, Monro, Brennan, & Bird, 2008), blending (Sarvan, Kramer, Bouwmeester, Dekker, & Verkerk, 2017;Weurding, Veldman, Veen, van der Aar, & Verstegen, 2001), and mincing/grinding (Englyst, Englyst, Hudson, Cole, & Cummings, 1999;Mishra, Monro, & Hedderley, 2008) were most frequently employed methods, due to the ease of use and the consistency in the size reduction achieved. An internationally harmonized static in vitro digestion protocol has been established for gastric and intestinal phases of digestion (Minekus et al, 2014) whereas a consensus on oral phase is still pending.…”