Use of Raman spectroscopy for diagnosis of disease in dental tissue

Timchenko, E. V.; Тимченко, П. Е.; Zherdeva, L. A.; Волова, Л. Т.; Burda, A. G.

doi:10.1364/jot.83.000313

Cited by 3 publications

(5 citation statements)

References 7 publications

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“…The spectral changes found in this work that occur in periodontitis do not occur in other widespread dental diseases (e.g., caries). We previously carried out studies [27] that showed a decrease in the concentration of ions (PO4) 3-in caries, as also shown in [28].…”

Section: Discussionmentioning

confidence: 84%

Raman Spectroscopy of Changes in the Tissues of Teeth with Periodontitis

et al. 2020

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The results of experimental studies of the tissues of teeth with periodontitis, using the Raman spectroscopy method, are presented in this work. Spectral changes in the tissues of teeth with periodontitis were identified, and the results can be used for the correction of treatment of this disease in dental practice. Criteria for the noninvasive diagnosis of periodontitis, based on changes in tooth enamel spectral properties, were developed.

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Section: Discussionmentioning

confidence: 84%

Raman Spectroscopy of Changes in the Tissues of Teeth with Periodontitis

et al. 2020

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“…The various other phosphate vibrations observed are the ν2 bending mode (430-456 cm −1 ), the ν3 asymmetric stretching mode (1,030-1,100 cm -1 ), and the ν4 asymmetric bending vibrations (530-645 cm −1 ). [8,13,14] Type-B carbonate substitution of the phosphate groups by carbonate is evidenced by a distinct peak at~1,070 cm −1 . [8,9,14] The intensity of the 960 cm −1 phosphate band was divided with the -CH 2 band at~2,940 cm −1 to calculate the degree of mineralization or mineral-to-matrix ratio of the different types of tissues.…”

Section: Discussionmentioning

confidence: 99%

“…[8,13,14] Type-B carbonate substitution of the phosphate groups by carbonate is evidenced by a distinct peak at~1,070 cm −1 . [8,9,14] The intensity of the 960 cm −1 phosphate band was divided with the -CH 2 band at~2,940 cm −1 to calculate the degree of mineralization or mineral-to-matrix ratio of the different types of tissues. [11,15,16] The use of this δCH 2 bending band was preferred to the amide I band mainly used in bone studies [8,17] as there are some interferences with the pMMA -C =O vibration.…”

Section: Discussionmentioning

confidence: 99%

“…An increase of the mineral-to-matrix ratio indicates a more mineralized matrix. [8,9,14,17] Mineral crystallinity is quantifiable by measuring the peak width at halfmaximum intensity of the bandwidth of the PO 4 3− peak at~960 cm −1 . A reduced mineral crystallinity, corresponding to a narrowing in the bandwidth of phosphate peak, indicates an increase in the mineral size and an increase in mineralization.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, we confirm by spectrum analysis and imaging what has previously been presented for dental tissues. [5,11,14,18,[20][21][22] Enamel is the hardest, higher crystalline tissue of the body, its matrix contains less than 5% organic matter, and it is poorly hydrated. This was evidenced on the spectra and on the mapping of the coronal dentine-enamel junction.…”

Section: F I G U R Ementioning

confidence: 99%

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Raman spectroscopic analysis and imaging in two cases of benign cementoma: Comparison with dental and bone tissues

Chappard

Guillaume

Teman³

et al. 2020

J Raman Spectroscopy

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Benign cementoma is a rare tumor of the jaws whose origin is still debating. The tumor may derive from the dental cementum or the bone surrounding the socket. We have studied the profiles of enamel, dentine, and cementum from normal teeth and alveolar and cortical bone of the mandible. Raman spectroscopy was used to compare the spectra of these tissues embedded in poly(methylmethacrylate) from 300 to 3,100 cm−1. The degree of mineralization, carbonate‐to‐phosphate ratio, and crystallinity were derived. Tissue hydration was determined by measuring the ratio of the 813 cm−1 band of methacrylate to the 960 cm−1 band of phosphate. Raman spectroscopic imaging was obtained on large tissue areas with a bandwidth from 500 to 1,620 cm−1. Maps of the different peak ratio were obtained to analyze the mineral and organic phases (crystallinity, carbonate‐to‐phosphate, degree of mineralization) together with tissue hydration. Cementomas appeared highly heterogeneous, and their matrix possessed histological and spectroscopic characteristics that resemble highly calcified woven bone with a poor organization of the hydroxyapatite crystals.

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