Use of RAPD analysis to assess the threat of interspecific hybridization to the critically endangered Polemonium kiushianum in Japan

Matoba, Hideyuki; Inaba, Kazufumi; Nagano, Katsuya; Uchiyama, Hiroshi

doi:10.1007/s10265-010-0353-2

Cited by 8 publications

(7 citation statements)

References 16 publications

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“…Similar cases have been reported, indicating that hybrids of Polemonium kiushianum and non-indigenous horticultural congeners are not detected in the semi-natural grasslands of Mt. Aso, Japan (Matoba et al 2011). However, interspecific hybrids are often found in the mixed zones where both parental species grow (Hasegawa et al 2006;Parsons & Hermanutz 2006;Kobayashi et al 2007;Kothera et al 2007;Yamamoto et al 2018).…”

Section: Discussionsupporting

confidence: 74%

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Ability of the endangered species Papaver fauriei to produce hybrids with a cultivated poppy (Papaver sp.)

Yamagishi

Onuma

Kondo

et al. 2018

Plant Species Biology

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Papaver fauriei is an endemic and endangered species that grows only on the gravelly alpine slopes of Mt. Rishiri, Japan. Cultivated poppy (Papaver sp.), the species name of which is unknown, has been introduced to the natural habitats of P. fauriei through human activities. Because the appearance and internal transcribed spacer (ITS) sequences of these two poppies are highly similar, it is of concern that they could produce hybrids in their natural habitats. Thus, first, the ability of these two poppies to produce hybrids was analyzed by artificial fertilization in this study. A large number of seeds were produced by reciprocal crosses between P. fauriei and the cultivated poppy, comparable with the number of seeds obtained by self‐ or cross‐fertilization of P. fauriei or the cultivated poppy. In addition, high germination was observed for seeds obtained from crosses between the two poppies, and deleterious phenotypes, such as albinism and dwarfism, were not detected in the F1 generation. These results indicate that after pollination, there is no reproductive isolation between the two poppies. Second, we sequenced the internal transcribed spacer (ITS) region of 240 poppy individuals collected from the gravelly alpine slopes of Mt. Rishiri, and 66 showed the sequence of P. fauriei, whereas 174 showed the sequence of the cultivated poppy. However, the ITS sequence that confirms hybridism between the two poppies was not detected in these individuals, indicating that hybridization of P. fauriei and the cultivated poppy rarely occurs under natural conditions. Unknown mechanism(s) appear to prevent cross‐pollination between the two poppies.

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Section: Discussionsupporting

confidence: 74%

“…Aso, Japan (Matoba et al . ). However, interspecific hybrids are often found in the mixed zones where both parental species grow (Hasegawa et al .…”

Section: Discussionmentioning

confidence: 97%

Ability of the endangered species Papaver fauriei to produce hybrids with a cultivated poppy (Papaver sp.)

Yamagishi

Onuma

Kondo

et al. 2018

Plant Species Biology

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“…RAPD analysis permits a study of genetic differences at a finer scale than that often obtained using nuclear and mitochondrial DNA sequencing and it allows for the study of the whole genome rather than only a restricted part. RAPD markers have been in use for ∼20 years and are widely applied in studies of population genetics, hybridization, genome mapping, species identifications and phylogenetics of bacteria, plants and animals (Matoba et al. 2001; Jones et al.…”

Section: Methodsmentioning

confidence: 99%

Connectivity, small islands and large distances: the Cellana strigilis limpet complex in the Southern Ocean

et al. 2011

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The Southern Ocean contains some of the most isolated islands on Earth, and fundamental questions remain regarding their colonization and the connectivity of their coastal biotas. Here, we conduct a genetic investigation into the Cellana strigilis (limpet) complex that was originally classified based on morphological characters into six subspecies, five of which are endemic to the New Zealand (NZ) subantarctic and Chatham islands (44-52°S). Previous genetic analyses of C. strigilis from six of the seven island groups revealed two lineages with little or no within-lineage variation. We analysed C. strigilis samples from all seven island groups using two mitochondrial (COI and 16S), one nuclear (ATPase β) and 58 loci from four randomly amplified polymorphic DNA markers (RAPDs) and confirmed the existence of two distinct lineages. The pronounced genetic structuring within each lineage and the presence of private haplotypes in individual islands are the result of little genetic connectivity and therefore very high self-recruitment. This study supports the significance of the subantarctic islands as refugia during the last glacial maximum and adds to the knowledge of contemporary population connectivity among coastal populations of remote islands in large oceans and the distance barrier to gene flow that exists in the sea (despite its continuous medium) for most taxa.

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“…Among the variety of molecular marker techniques available, RAPD (Welsh and McClelland, 1990;Williams et al, 1990) has been extensively used for DNA fingerprinting of genotypes because of its technical simplicity, the low cost and no requirement for sequence information, a demand for small quantities of DNA, and because it can produce abundant polymorphic markers (Wolfe and Liston, 1998). Despite criticism, RAPD analyses are now extensively used for estimating genetic relationships among closely related populations or species and providing molecular evidence concerning the hybrid origin of plants (Smith et al, 1996;Baumel et al, 2003;González-Rodríguez et al, 2004;Mehetre et al, 2004aMehetre et al, , 2004bDivakaran et al, 2006;Singh et al, 2007;Ali et al, 2008;Srivastava et al, 2010;Matoba et al, 2011;Mukherjee et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Interspecific in vitro hybridization in genus Centaurium and identification of hybrids via flow cytometry, RAPD, and secondary metabolite profiles

Banjanac¹,

Šiler²,

Škorić³

et al. 2014

Turk J Bot

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Most of the species from the genus Centaurium Hill readily hybridize between themselves in natural habitats. Artificial interspecific hybrid plants were obtained under in vitro conditions using reciprocal crosses between 4 species: Centaurium erythraea, C. littorale, C. maritimum, and C. pulchellum. Among 76 crossing experiments performed, 3 types of crosses produced seeds and gave 20 interspecific hybrid plants. Nuclear DNA quantification, fingerprinting of molecular markers, and determination of variation in main secondary metabolite content were employed to confirm their hybrid nature. All 3 approaches were capable of detecting hybrid plants. RAPD molecular markers proved to be the most reliable, always placing the hybrids between their parents in dendrograms, while secoiridoid glycoside content also showed intermediate profiles in the hybrid plants in relation to the parents. Hence, each of the techniques employed can be recommended for elucidating the mechanisms of interspecific hybridization in natural populations of Centaurium species in further studies. This could help to comprehend their breeding system and recognize interspecific hybridization as one of the crucial steps in polyploid formation and speciation in this genus.

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Use of RAPD analysis to assess the threat of interspecific hybridization to the critically endangered Polemonium kiushianum in Japan

Cited by 8 publications

References 16 publications

Ability of the endangered species Papaver fauriei to produce hybrids with a cultivated poppy (Papaver sp.)

Ability of the endangered species Papaver fauriei to produce hybrids with a cultivated poppy (Papaver sp.)

Connectivity, small islands and large distances: the Cellana strigilis limpet complex in the Southern Ocean

Interspecific in vitro hybridization in genus Centaurium and identification of hybrids via flow cytometry, RAPD, and secondary metabolite profiles

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