Use of reagent-impregnated (“Patho-Tec”) test papers in the identification of Enterobacteriaceae and similar bacteria

Narayan, K. G.; Guiñée, P. A. M.; Mossel, D. À. A.

doi:10.1007/bf02045549

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…Thus, excellent results were obtained with the Voges-Proskauer, the phenylalanine, the oxidase, and, with one exception, with the indole test. Our results agree generally with those of other authors (2,7,9,10,14) as regards the accurate and consistent results obtained with the oxidase and phenylalanine tests. The same is only partially true for our good results with Voges-Proskauer strips.…”

Section: Discussionsupporting

confidence: 94%

Comparative Study of the Efficacy of Seven Paper-Reagent Strips and Conventional Biochemical Tests in Identifying Gram-negative Organisms

Matsen¹,

Sherris²

1969

Applied Microbiology

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Gram-negative organisms were tested with commercially available reagentimpregnated strips (PATHO-TEC). Of the 291 strains, all were tested by using seven paper tests and their conventional counterparts. Excellent correlation was obtained with the oxidase, phenylalanine-deaminase, and Voges-Proskauer tests. Indole tests made on liquid medium cultures also gave complete correlation, but some false-negative results with indole-positive Proteus strains were obtained when growth from solid medium was tested by the strip method. Paper strip urease tests were positive within 2 hr with all Klebsiella and some Serralia, Herellea, and Citrobacter strains as well as with Proteus strains. Approximately 15% of citrate strip test results differed from those of the conventional tests, and reproducibility was poor on retest. The lysine decarboxylase strip test showed a number of discrepancies and posed problems of interpretation and readability. Paper reagent strip methods are simple and convenient and merit further development to increase the specificity of those which depend on pH change up to that achieved with the Voges-Proskauer, oxidase, phenylalanine, and indole methods.

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Section: Discussionsupporting

confidence: 94%

Comparative Study of the Efficacy of Seven Paper-Reagent Strips and Conventional Biochemical Tests in Identifying Gram-negative Organisms

Matsen¹,

Sherris²

1969

Applied Microbiology

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“…The first commercial kit with tests for specific enzymes con-tained Patho-Tec paper strips (172), a method that evolved to the Micro-ID system (15) for the identification of clinically important gram-negative rods, mainly members of the Enterobacteriaceae. The Micro-ID kit included tests for ␤-galactosidase, cytochrome oxidase, lysine and ornithine decarboxylases, tryptophanase, and urease.…”

Section: Introductionmentioning

confidence: 99%

Use of Enzyme Tests in Characterization and Identification of Aerobic and Facultatively Anaerobic Gram-Positive Cocci

Bascomb¹,

Manafi

1998

Clin Microbiol Rev

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SUMMARY The contribution of enzyme tests to the accurate and rapid routine identification of gram-positive cocci is introduced. The current taxonomy of the genera of aerobic and facultatively anaerobic cocci based on genotypic and phenotypic characterization is reviewed. The clinical and economic importance of members of these taxa is briefly summarized. Tables summarizing test schemes and kits available for the identification of staphylococci, enterococci, and streptococci on the basis of general requirements, number of tests, number of taxa, test classes, and completion times are discussed. Enzyme tests included in each scheme are compared on the basis of their synthetic moiety. The current understanding of the activity of enzymes important for classification and identification of the major groups, methods of testing, and relevance to the ease and speed of identification are reviewed. Publications describing the use of different identification kits are listed, and overall identification successes and problems are discussed. The relationships between the results of conventional biochemical and rapid enzyme tests are described and considered. The use of synthetic substrates for the detection of glycosidases and peptidases is reviewed, and the advantages of fluorogenic synthetic moieties are discussed. The relevance of enzyme tests to accurate and meaningful rapid routine identification is discussed.

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3 Enzyme Tests in Bacterial Identification

Bascomb

1988

Methods in Microbiology

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Use of reagent-impregnated (“Patho-Tec”) test papers in the identification of Enterobacteriaceae and similar bacteria

Cited by 5 publications

References 10 publications

Comparative Study of the Efficacy of Seven Paper-Reagent Strips and Conventional Biochemical Tests in Identifying Gram-negative Organisms

Comparative Study of the Efficacy of Seven Paper-Reagent Strips and Conventional Biochemical Tests in Identifying Gram-negative Organisms

Use of Enzyme Tests in Characterization and Identification of Aerobic and Facultatively Anaerobic Gram-Positive Cocci

3 Enzyme Tests in Bacterial Identification

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