Microfermentation tests for members of the family
Enterobacteriaceae
were devised by using agar solutions in disposable, multi-welled, plastic trays. The tests could be made directly from isolated colonies picked from agar plates and represented a considerable saving in time, labor, and materials over the conventional methods. Tests were formulated for determining carbohydrate fermentations, citrate utilization, motility, amino acid decarboxylation, and production of H
2
S, indole, urease, and acetyl-methyl-carbinol.