Use of real-time PCR to detect <i>Mycoplasma haemofelis</i> and ‘<i>Candidatus</i> Mycoplasma haemominutum’ in the saliva and salivary glands of haemoplasma-infected cats

Dean, Rachel; Helps, Christopher R; Jones, Timothy J.; Tasker, Séverine

doi:10.1016/j.jfms.2007.12.007

Cited by 54 publications

(48 citation statements)

References 15 publications

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“…It is postulated that transmissions can also occur via contact with contaminated saliva, feces and/or urine. Furthermore, it's possible that each feline hemoplasma may have a different transmission route (Dean et al 2008).…”

Section: Resultsmentioning

confidence: 99%

Frequency and hematological alterations of different hemoplasma infections with retrovirusis co-infections in domestic cats from Brazil

et al. 2016

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ABSTRACT.-Firmino F.P., Aquino L.C., Marçola T.G., Bittencourt M.V., Mycoplasma haemofelis is the agent of feline infectious anemia, although Candidatus M. haemominutum can also be associated. This study evaluated the frequency and hematological alterations caused by hemoplasma infections and co-infections with FeLV, FIV and Toxoplasma gondii in domestic cats from two distinct areas (urban -G1 and periurban -G2) of Brasília, Brazil. One hundred cats were evaluated, 51 from the G1 area and 49 from G2. No cats were positive for T. gondii. Hemoplasma infection was diagnosed in 33% cats from G1 and 32.6% from G2 (p>0.05). In G1 35.3% of the positive cats were infected with Mycoplasma haemofelis, 47.06% with Candidatus Mycoplasma haemominutum and 17.64% with mixed hemoplasma species infection; 12.5% of the cats identified as PCR positive in G2 were infected with Mycoplasma haemofelis, 18.75% with Candidatus Mycoplasma haemominutum and 68.75% with mixed infection. Cats from the periurban area had higher mixed hemoplasmas infection rates than those from urban area, and most of them were asymptomatic carriers. Cytology results were positive in only 5% of cats from G1. Mycoplasma haemofelis infected cats had normocytic normochromic anemia while the cats infected with Candidatus Mycoplasma haemominutum or with both species did not. 37.2% of G1 cats were co-infected with Mycoplasma haemofelis and FeLV, and presented lower PCV and hemoglobin concentration than those infected only with Mycoplasma haemofelis. The co--infection with Candidatus Mycoplasma haemominutum and FeLV produced lower WBC, segmented cells and platelets, and increased total protein concentration.

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Section: Resultsmentioning

confidence: 99%

Frequency and hematological alterations of different hemoplasma infections with retrovirusis co-infections in domestic cats from Brazil

et al. 2016

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“…3,5,19 However, the mode of transmission of hemoplasma infection in field cases has not been confirmed, although transmission by fleas and ticks has been investigated. 21,23 In the present study, presence of coinfection with other vector-borne pathogens was significantly associated with a positive hemoplasma qPCR result.…”

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Prevalence of Hemotropic Mycoplasmas in Healthy and Unhealthy Cats and Dogs in Spain

Roura

Peters

Altet³

et al. 2010

J VET Diagn Invest

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Abstract. The aims of the present study were to determine the prevalence of hemoplasmas in cats and dogs from the Barcelona area of Spain with the use of species-specific quantitative polymerase chain reaction (qPCR) assays and to evaluate any associations between hemoplasma infection, clinical presentation, and vector-borne infections. Blood samples from cats (191) and dogs (182) were included and were classified as healthy (149) or unhealthy (224). Ethylenediamine tetra-acetic acid blood samples underwent DNA extraction and qPCR analysis. Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum', and 'Candidatus Mycoplasma turicensis' were detected in cats, whereas Mycoplasma haemocanis and 'Candidatus Mycoplasma haematoparvum' were detected in dogs, with prevalences of 3.7%, 9.9%, 0.5%, 14.3%, and 0.6%, respectively. In cats, no association between hemoplasma infection and health status, age, breed, presence of anemia, Feline leukemia virus status, and other vector-borne infections was found, but outdoor access (P 5 0.009), male sex (P 5 0.01), and Feline immunodeficiency virus status (P , 0.001) were significantly associated with hemoplasma infection. In dogs, sex, age, health status, presence of anemia, and breed were not significantly associated with hemoplasma infection, but a significant association was found between hemoplasma infection and vector-borne infections (P , 0.001). The present report documents the occurrence of feline 'Candidatus M. turicensis' and canine 'Candidatus M. haematoparvum' infections in Spain.

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“…Mycoplasma haemominutum" and "Ca. Mycoplasma turicensis" have been detected in the saliva of infected cats (6,35). On the other hand, the recent discovery of "Ca.…”

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Worldwide Occurrence of Feline Hemoplasma Infections in Wild Felid Species

et al. 2007

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While hemoplasma infections in domestic cats are well studied, almost no information is available on their occurrence in wild felids. The aims of the present study were to investigate wild felid species as possible reservoirs of feline hemoplasmas and the molecular characterization of the hemoplasma isolates. Blood samples from the following 257 wild felids were analyzed: 35 Iberian lynxes from Spain, 36 Eurasian lynxes from Switzerland, 31 European wildcats from France, 45 lions from Tanzania, and 110 Brazilian wild felids, including 12 wild felid species kept in zoos and one free-ranging ocelot. Using real-time PCR, feline hemoplasmas were detected in samples of the following species: Iberian lynx, Eurasian lynx, European wildcat, lion, puma, oncilla, Geoffroy's cat, margay, and ocelot. "Candidatus Mycoplasma haemominutum" was the most common feline hemoplasma in Iberian lynxes, Eurasian lynxes, Serengeti lions, and Brazilian wild felids, whereas "Candidatus Mycoplasma turicensis" was the most prevalent in European wildcats; hemoplasma coinfections were frequently observed. Hemoplasma infection was associated with species and free-ranging status of the felids in all animals and with feline leukemia virus provirus-positive status in European wildcats. Phylogenetic analyses of the 16S rRNA and the partial RNase P gene revealed that most hemoplasma isolates exhibit high sequence identities to domestic cat-derived isolates, although some isolates form different subclusters within the phylogenetic tree. In conclusion, 9 out of 15 wild felid species from three different continents were found to be infected with feline hemoplasmas. The effect of feline hemoplasma infections on wild felid populations needs to be further investigated.

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Use of real-time PCR to detect Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’ in the saliva and salivary glands of haemoplasma-infected cats

Cited by 54 publications

References 15 publications

Frequency and hematological alterations of different hemoplasma infections with retrovirusis co-infections in domestic cats from Brazil

Frequency and hematological alterations of different hemoplasma infections with retrovirusis co-infections in domestic cats from Brazil

Prevalence of Hemotropic Mycoplasmas in Healthy and Unhealthy Cats and Dogs in Spain

Worldwide Occurrence of Feline Hemoplasma Infections in Wild Felid Species

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