Use of real-time polymerase chain reaction to identify Entamoeba histolytica in schoolchildren from northwest Mexico

Aguayo-Patrón, Sandra V; Castillo-Fimbres, Reyna; Quihui‐Cota, Luis; Barca, Ana María Calderón de la

doi:10.3855/jidc.9350

Cited by 4 publications

(4 citation statements)

References 21 publications

(34 reference statements)

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“…The convenience non-probabilistic sampling study was carried out collecting data from 2016 to 2018 in northwestern Mexico (Sonora State), a desertic area with dry climate, previously described [ 2 ]. Children under 18 years old with a diagnosis of T1D or CD were recruited at the external consultation of the Children Sonora State Hospital and the Mexican Social Security Institute (HIES and IMSS).…”

Section: Methodsmentioning

confidence: 99%

“…The Blastocystis spp. identification was done according to Grabensteiner and Hess [ 12 ], starting with an initial hold at 95 °C for 15 min, followed by 40 cycles at 94 °C for 30 s, 60 °C for 30 s and 72 °C for 60 s. For the E. histolytica, the conditions were the same previously described by ourselves [ 2 ]. For G. lamblia , the conditions were an initial 94 °C for 5 min, followed by 35 cycles at 94 °C for 30 s, 58 °C for 90 s, 72 °C for 90 s and 72 °C for 10 min [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

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Enteric parasitic infection disturbs bacterial structure in Mexican children with autoantibodies for type 1 diabetes and/or celiac disease

et al. 2020

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Background Intestinal bacterial dysbiosis and increased gut permeability are associated with higher risk of developing type 1 diabetes (T1D) or celiac disease (CD). There is a lack of information on parasitism involved in gut disturbance of predisposed children. We evaluated the effect of enteropathogenic parasites ( Cryptosporidium spp., Cyclospora spp. G. lamblia , and Blastocystis spp.) on the bacterial structure of feces from children with autoantibodies for T1D or CD. Participants included 37 children under 18 years of age, from whom stools were analyzed for enteric parasites by qPCR and 22/37 for bacterial profile by sequencing the V3–V4 region of the 16s rRNA gene. Dietary, clinical, and socioeconomic data was recorded. Results Pathogens parasitized 28/37 participants, Cryptosporidium spp. was the most prevalent (62.2%), followed by both Cyclospora cayetanensis and Blastocystis spp (37.8%). There were no dietary differences ( p > 0.05) attributable to parasitism. Co-infected participants with Cryptosporidium and Cyclospora did not differ (p = 0.064) from non-infected participants in bacterial alpha phylogenetic diversity. The same parasites’ co-infection was associated with a decreased abundance of the Ruminococaceae (p = 0.04) and Verrucomicrobioceae families, of the Akkermansia genus (p = 0.009). There was a lower Firmicutes/Bacteroidetes ratio (p = 0.02) in infected than in uninfected participants. Conclusions Cryptosporidium and Cyclospora affected the bacterial structure at family and genus levels, decreasing the ratio between Firmicutes and Bacteroidetes in children with auto-antibodies for T1D or CD, which could increase the risk of illness onset.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Enteric parasitic infection disturbs bacterial structure in Mexican children with autoantibodies for type 1 diabetes and/or celiac disease

et al. 2020

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“…The TaqMan method, which targets the 18S rRNA gene, is more specific than the SYBR Green method for the detection of amoebiasis, according to a comparison of real-time PCR using different probes. Furthermore, they discovered that E. histolytica can be found in clinical samples containing very few parasites using probe-based real-time PCR techniques that are undetectable with conventional PCR (59).…”

Section: Real-time Pcr Methodsmentioning

confidence: 99%

Evaluation of Different Techniques in Laboratory Diagnosis of Intestinal Amoebiasis

Jannati,

Tavakoli Pasand,

Garedaghi

2024

Int J Med Parasitol Epidemiol Sci

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Entamoeba histolytica is an intestinal protozoan disease that causes amoebiasis, which is a health problem in several developing countries that can lead to 100000 deaths annually. This parasite is the third cause of death in tropical regions with poor sanitary conditions. An essential part of the treatment of patients is the detection of the pathogenic E. histolytica and its differentiation from non-pathogenic Entamoeba spp. Because microscopy and antigen detection techniques are inexpensive and readily available, these techniques are commonly used to diagnose amoebiasis. Rapid tests and different ELISAs for antigen detection are some of the more modern and sensitive methods, and some diagnostic methods are not able to distinguish different species of Entamoeba. Techniques for molecular detection are highly specific and sensitive. However, utilizing molecular methods as the usual diagnostic method becomes difficult due to their high cost in most endemic areas. For the diagnosis of intestinal amoebiasis, there is still a need for highly sensitive and specific tests that are quick and affordable to use, especially in developing countries where this disease is common.

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“…Antibody testing can be used to diagnose amoebiasis, although the majority of patients with intestinal amoebiasis have been exposed to Entamoeba histolytica and have generated IgG antibodies to this parasite, which can last for a long period. As a result of the difficulties in distinguishing between past and current illnesses, exact diagnosis utilising available IgG antibody detection techniques is a problem [14,15]. Stool culture with isoenzyme analysis was commonly used as reference method to differentiate between E. histolytica and E. dispar.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Microscopy, Boeck and Drbolav’s Stool Culture Medium and Bichro-latex Antibody Test with A Reference Elisa Antigen Test for E. histolytica Diagnosis in Calabar, Nigeria

Onosakponome¹,

Ohanu²,

U.³

2021

MRJI

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Objective: Amoebiasis is a parasitic infection caused by E.histolytica, accurate diagnosis of E. histolytica is important in the treatment of amoebiasis and to avoid preventable costs. The study objective is to compare different diagnostic methods used in the diagnosis of amoebiasis for detection of E.histolytica parasite. Materials and Methods: Faecal and serum specimens of 200 patients defined as symptomatic (diarrhea and dysentery) and asymptomatic (a case history of E.histolytica infection) was used for the study .Stool specimen was examined with microscopy, cultured in Boeck and drbolav’s medium and anti-E. histolytica antibodies were investigated using a latex slide test. Stool samples were also examined by immunoassay methods for specific antigens which is the reference standard for comparison. Result: Two hundred (200) samples examined for E.histolytica parasite 12(6.0%) were positive in in microscopy,34(17%) in bichro-latex antibody test and 6(3.0%) in Boeck and drbolav’s culture medium. The three test methods showed significant detection of E.histolytica parasite(p<0.05). Microscropic method detected 100% of E.histolytica infection in symptomatic patients and Boeck and Drbohlav’s culture medium detected 33.3%. However, the method of diagnosis is not associated with the diagnosis E.histolytica infection in asymptomatic and symptomatic Patients(p>0.05).The diagnostic precision of the microscopy diagnostic method showed that sensitivity was 40.2%, specificity was 82.3%, PPV 39.6% and NPV 70.4% .The sensitivity was 86.6%, specificity was 70.6% PPV 87.6% and NPV 75.6% for bichro-latex antibody test and The sensitivity was 20.6 %, specificity was 50.6 %, PPV 34.6%, NPV 61.2% for Boeck and Drbohlav’s culture medium Conclusion: The result from the comparison of the three diagnostic methods for E.histolytica parasite indicate high sensitivity and specificity for bichro-latex antibody test when compared with the other methods. however, in areas were molecular technology such as Polymerase chain reaction and enzyme linked immunosorbent assay is not available, bichro-latex antibody assay is recommended. although,microscopic examination can be used in diagnosis of amoebiasis in geographical areas with technological limitation in health.

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Use of real-time polymerase chain reaction to identify Entamoeba histolytica in schoolchildren from northwest Mexico

Cited by 4 publications

References 21 publications

Enteric parasitic infection disturbs bacterial structure in Mexican children with autoantibodies for type 1 diabetes and/or celiac disease

Enteric parasitic infection disturbs bacterial structure in Mexican children with autoantibodies for type 1 diabetes and/or celiac disease

Evaluation of Different Techniques in Laboratory Diagnosis of Intestinal Amoebiasis

Comparison of Microscopy, Boeck and Drbolav’s Stool Culture Medium and Bichro-latex Antibody Test with A Reference Elisa Antigen Test for E. histolytica Diagnosis in Calabar, Nigeria

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