Use of reference pools to compare the qualitative and quantitative determination of polychlorinated biphenyls by packed and capillary gas chromatography with electron capture detection. Part 1. Serum

Abstract

Serum for reference pools of in vivo polychlorinated biphenyls (PCBs) was obtained from four goats that had received one dose (100 mg kg-1) of a selected technical Aroclor (AR) (1016, 1242, 1254 or 1260) and were allowed to recover for 30 d. These pools were used to assess the differences in an analytical method that determines and quantifies PCBs using packed-column gas chromatography (PCGC) (quantified on the basis of mean mass percent. data for grouped PCB peaks) and capillary-column gas chromatography (CCGC) (quantified on the basis of percent. composition data for specific congeners). With CCGC, results were statistically significantly different (p less than or equal to 0.0002) from results with PCGC for ARs 1016, 1242 and 1254 but not for AR 1260 (p = 0.23). When comparing these gas chromatographic methods using bovine serum spiked in vitro with the same ARs at 17-25 p.p.b., it was found that the methods were not statistically significantly different for any of the ARs (p = 0.30-0.92). Levels of serum PCB determined by the two methods for 12 persons, divided into two groups according to exposure, were compared using the paired t-test. Group 1 consisted of three persons with dietary and/or environmental exposure; one with dietary and/or environmental exposure in addition to occupational exposure dating back 20 years. Group 2 consisted of eight persons with recent occupational exposure.(ABSTRACT TRUNCATED AT 250 WORDS)

Measurement of PCBs, DDE, and hexachlorobenzene in cord blood from infants born in towns adjacent to a PCB-contaminated waste site

“…Assuming that the sum of PCB congeners determined by capillary column accounts for 50 ±90% of the total PCBs calculated by the packed column method (Burse et al, 1990(Burse et al, , 1994Johansen et al, 1994;Liem et al, 1996;Erickson, 1997), these cord blood concentrations are roughly comparable to P PCB concentrations of between 1.3 and 2.3 ng/ ml cord serum, or approximately two -to fourfold greater than mean concentrations observed 12 ±18 years later in New Bedford. Consistent with this finding is the observation that U.S. population exposures to PCBs and DDT (or DDE) have generally declined since chemicals were banned from industrial use in the 1970s (Slorach and Vaz, 1985;Patterson et al, 1994;Noren et al, 1996).…”

“…However, comparing the New Bedford serum PCB and pesticide concentrations with those of earlier studies in which congener -specific analyses were not used is complicated by differences in analytic techniques. For example, although there are limited published data comparing the results of capillary and packed column analyses of the same samples, the sum of PCBs measured by capillary column congener -specific analyses has typically been estimated to account for between 50% and 90% of total PCBs measured by older techniques that relied on packed column analysis and Aroclor-based quantification methods (Burse et al, 1990(Burse et al, , 1994Johansen et al, 1994;Liem et al, 1996;Erickson, 1997).…”

Serum Concentrations of Various Environmental Contaminants and Their Relationship to Sex Steroid Concentrations and Phallus Size in Juvenile American Alligators

“…The quality control material was bovine serum previously spiked with three levels of analytes (Burse et al 1990); Gibco BRL calf serum (Life Technologies, Grand Island, NY). One quality control sample and one reagent blank were analyzed with every batch of 10 alligator samples.…”

A Correlation Study of Organochlorine Levels in Serum, Breast Adipose Tissue, and Gluteal Adipose Tissue among Breast Cancer Cases in India

“…The gas chromatography with electron capture detection was calibrated by repetitive analysis of standard mixtures of selected individual PCB congeners at six levels for the quantification of individual congeners. The analysis method for individual PCB congeners was a modified version of established methods (8)(9)(10)(11)(12)). …”