Use of resistant mutants to study the interaction of triton X-100 with Staphylococcus aureus

We previously reported that Triton X-100 (TRX) reduced methicillin resistance in Staphylococcus aureus, although the degree of reduction varied among strains. One of the biological effects of TRX on S. aureus cells was enhancement of lipoteichoic acid (LTA) release. We investigated the correlation between the amount of LTA released and the degree of reduction in methicillin resistance induced by TRX. The strains showing the greatest reduction of methicillin resistance released the largest amount of LTA, compared to those showing slight or moderate reduction. A mutant whose resistance was not affected by TRX did not increase its release of LTA. These findings suggest that LTA release is associated with a reduction in methicillin resistance in the presence of TRX.

Section: Introductionsupporting

confidence: 80%

Triton X-100-induced lipoteichoic acid release is correlated with the methicillin resistance inStaphylococcus aureus

Ohta

Komatsuzawa

Sugai

et al. 2000

“…During our characterization of this sensitizing e¡ect, we found that TRX stimulated lipoteichoic acid (LTA) release [9]. This ¢nding was also reported by Raychaudhuri and Chatterjee [11]. LTA is an amphiphilic molecule and anchored to the bacterial cell membrane [4].…”

Section: Introductionsupporting

confidence: 78%

Triton X-100-induced lipoteichoic acid release is correlated with the methicillin resistance in Staphylococcus aureus

Ohta

2000

We previously reported that Triton X‐100 (TRX) reduced methicillin resistance in Staphylococcus aureus, although the degree of reduction varied among strains. One of the biological effects of TRX on S. aureus cells was enhancement of lipoteichoic acid (LTA) release. We investigated the correlation between the amount of LTA released and the degree of reduction in methicillin resistance induced by TRX. The strains showing the greatest reduction of methicillin resistance released the largest amount of LTA, compared to those showing slight or moderate reduction. A mutant whose resistance was not affected by TRX did not increase its release of LTA. These findings suggest that LTA release is associated with a reduction in methicillin resistance in the presence of TRX.

“…The finding of low lysis induction by daptomycin in strain DU4916 can be considered as a tolerant response to a cell wall antibiotic [18], thus establishing a possible link between this phenomenon in staphylococci [19] and methicillin resistance. In accord with this, lower minimum inhibitory concentrations of vancomycin and D-cycloserine were noted in strain DU4916S than DU4916 [13].…”

Section: Discussionmentioning

confidence: 92%

“…It is not known how Triton X-100 stimulates S. aureus autolytic activity. Release of the putative autolysin inhibitor lipoteichoic acid may be involved, but it is only a partial explanation [18]. There is evidence for a variety of motifs in autolysin regulation in various bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…Triton X-100-stimulated autolysis was measured with some modifications of the method of Raychaudhuri and Chatterjee [18]. Cells were grown in the presence of Triton X-100, typically 0.01-0.05 % (vol/vol), and autolysis of cells washed in cold distilled water, was measured in 0.05 M potassium phosphate buffer pH 7.2 with shaking (200 r.p.m.)…”

Section: Measurements Of Autolytic Activitymentioning

confidence: 99%

See 1 more Smart Citation

Lower autolytic activity in a homogeneous methicillin-resistant Staphylococcus aureus strain compared to derived heterogeneous-resistant and susceptible strains

Gustafson

1989

It has been proposed that in addition to production of a penicillin-binding protein with low affinity for beta-lactam antibiotics, control of autolysin activity is involved in the mechanism of staphylococcal methicillin resistance. A homogeneous methicillin-resistant Staphylococcus aureus strain (DU4916) had lower rates of unstimulated, NaCl- and Triton X-100-stimulated autolysis, and daptomycin (LY146032)-induced lysis than a heterogeneous methicillin-resistant strain (DU4916-K7) and a methicillin-susceptible strain (DU4916S) derived from DU4916.