Use of Response Surface Methodology for the Rapid Optimization of Microbiological Media

Maddox, I. S.; Richert, S. H.

doi:10.1111/j.1365-2672.1977.tb00743.x

Cited by 78 publications

(34 citation statements)

References 5 publications

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“…One of the worthwhile technique to identify the explanatory variable in the system is response surface methodology (RSM) [11,12]. RSM can be used to evaluate the relative signi®cance of several factors.…”

Section: Introductionmentioning

confidence: 99%

Optimization of growth medium for the production of spores from Bacillus thuringiensis using response surface methodology

Liu

Tzeng

1998

Bioprocess Engineering

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The effects of cultivation medium compositions including tapioca, ®shmeal, CaCO 3 and (NH 4 ) 2 SO 4 for the growth of Bacillus thuringiensis YMB 96-1988 were accessed by using response surface methodology (RSM). The two-level (2 4±1 ) fractional factorial designs (FFD) which involve two concentrations of each nutrient, and the paths of steepest ascent were effective in searching for the major factors of the bacteria growth. This allows the ®tting of a ®rst order linear model to the data. In this study, supplementary CaCO 3 showed a negative effect on the spore production based on the ®rst order regression coef®cients derived from SAS programme. Subsequently, a 2 3 central composite design (CCD) was used for allocation of treatment combinations. Preliminary studies showed that tapioca and ®shmeal is believed to be the major factors for the growth of B. thuringiensis. Estimated optimum compositions for the production of spores by B. thuringiensis are as follows: tapioca, 5.01%; ®shmeal 5.86%; (NH 4 ) 2 SO 4 0.06% and resulted in a maximum spore count of 8.56´10 8 /ml was obtained. This value is close to the 8.35´10 8 /ml spore density as counted from actual experimental observations.

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Section: Introductionmentioning

confidence: 99%

Optimization of growth medium for the production of spores from Bacillus thuringiensis using response surface methodology

Liu

Tzeng

1998

Bioprocess Engineering

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“…Moderate, but economically reasonable yields of gibberellin were produced on molasses, sulfite liquors, and skimmed milk (FRG Patent 1081402; Maddox, 1977). The Soviet workers have successfully used plant oils, e.g.…”

Section: Nutrient Media and Environmental Factors For Gibbereuin Prodmentioning

confidence: 99%

Fungal Gibberellin Production

Brueckner

Blechschmidt

Sembdner

et al. 1989

Biotechnology of Vitamins, Pigments and Growth Factors

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“…RSM is a technique for studying the effect of several factors acting together and affecting the responses by varying them in a number of experiments [2]. RSM had been successfully applied in the optimization of medium composition for the production of glucosyltransferase by Aspergillus niger [3], optimization of growth medium for the production of CGTase from Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

MEDIUM OPTIMIZATION FOR THE PRODUCTION OF BIOMASS BY Cunninghamella sp. 2A1 USING RESPONSE SURFACE METHODOLOGY

Sulaiman

Hamid

Yusoff

2017

AJSTD

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A statistical design approach has been used to optimize the production of biomass by Cunninghamella sp. 2A1, evaluated based on lipidless biomass. A 2 3 full factorial central composite design (CCD) was chosen to study the combined effects of three factors; ammonium tartrate, peptone and glucose concentrations. The p-value for each factors was < 0.05 suggesting that these factors have significant effect on the production of lipidless biomass. The production is represented by a linear model with p-value < 0.0001. The optimized medium consists of 3.86 g/l ammonium tartrate, 55.84 g/l glucose and 7.73 g/l peptone predicted 16.83 g/l lipidless biomass. Results from four replications based on the optimized medium produced 18.48 g/l lipidless biomass, which are in close agreement with the predicted value. The coefficient for glucose was the highest indicating it as the most significant factor affecting lipidless biomass production.

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Use of Response Surface Methodology for the Rapid Optimization of Microbiological Media

Cited by 78 publications

References 5 publications

Optimization of growth medium for the production of spores from Bacillus thuringiensis using response surface methodology

Optimization of growth medium for the production of spores from Bacillus thuringiensis using response surface methodology

Fungal Gibberellin Production

MEDIUM OPTIMIZATION FOR THE PRODUCTION OF BIOMASS BY Cunninghamella sp. 2A1 USING RESPONSE SURFACE METHODOLOGY

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