Use of reversed phase high pressure liquid cromatography for the physicochemical and thermodynamic characterization of oxyresveratrol/β-cyclodextrin complexes

Rodríguez-Bonilla, Pilar; López‐Nicolás, José Manuel; García-Carmona, Francisco

doi:10.1016/j.jchromb.2010.04.016

Cited by 29 publications

(19 citation statements)

References 33 publications

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“…There are a few reports concerning the stability of OR. OR possesses low bioavailability and is easily oxidized by prooxidant agents (42).…”

Section: Discussionmentioning

confidence: 99%

“…This may be affected by the ME formula with a permeation enhancer in reducing the diffusion barrier (32,40,41). As a commonly used transdermal enhancer, isopropyl myristate has been supposed to be able to increase permeation by fluidizing intercellular lipids in the stratum corneum or to increase drug solubility at the lipid part of the stratum corneum (42,43). In addition, surfactant including Tween 80 enhanced the flux of drug across the skin by incorporating in skin lipid bilayer and causing more permeable arrangement or increasing the fluidity of the membrane (44).…”

Section: Discussionmentioning

confidence: 99%

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Microemulsion-Based Oxyresveratrol for Topical Treatment of Herpes Simplex Virus (HSV) Infection: Physicochemical Properties and Efficacy in Cutaneous HSV-1 Infection in Mice

et al. 2012

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Abstract. The physicochemical properties of the optimized microemulsion and the permeating ability of oxyresveratrol in microemulsion were evaluated, and the efficacy of oxyresveratrol microemulsion in cutaneous herpes simplex virus type 1 (HSV-1) infection in mice was examined. The optimized microemulsion was composed of 10% w/w of isopropyl myristate, 35% w/w of Tween 80, 35% w/w of isopropyl alcohol, and 20% w/w of water. The mean particle diameter was 9.67±0.58 nm, and the solubility of oxyresveratrol in the microemulsion was 196.34±0.80 mg/ml. After accelerated and long-term stability testing, the microemulsion base and oxyresveratrol-loaded microemulsion were stable. The cumulative amount of oxyresveratrol permeating through shed snake skin from microemulsion at 6 h was 93.04 times compared to that of oxyresveratrol from Vaseline, determined at 20% w/w concentration. In cutaneous HSV-1 infection in mice, oxyresveratrol microemulsion at 20%, 25%, and 30% w/w, topically applied five times daily for 7 days after infection, was significantly effective in delaying the development of skin lesions and protecting from death (p<0.05) compared with the untreated control. Oxyresveratrol microemulsion at 25% and 30% w/w was significantly more effective than that of 30% w/w of oxyresveratrol in Vaseline (p<0.05) and was as effective as 5% w/w of acyclovir cream, topically applied five times daily (p>0.05). These results demonstrated that topical oxyresveratrol microemulsion at 20-30% w/w was suitable for cutaneous HSV-1 mouse infection.

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“…There are a few reports concerning the stability of OR. OR possesses low bioavailability and is easily oxidized by prooxidant agents (42).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Microemulsion-Based Oxyresveratrol for Topical Treatment of Herpes Simplex Virus (HSV) Infection: Physicochemical Properties and Efficacy in Cutaneous HSV-1 Infection in Mice

et al. 2012

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“…This effect is clearly the result of by the encapsulation of phenolic compounds (reported in ascocarps of T. claveryi ), some of which may be substrates of tyrosinase. A similar effect of CDs (complexing phenols and preventing the browning of plant extracts) has been described by our group . Tyrosinase (EC 1.14.18.1) uses molecular oxygen to catalyze the oxidation of monophenols to their corresponding o ‐diphenols and their subsequent oxidation to o ‐quinones .…”

Section: Resultsmentioning

confidence: 54%

Mycelium growth stimulation of the desert truffleTerfezia claveryichatin by β‐cyclodextrin

López‐Nicolás

Pérez‐Gilabert

García-Carmona

et al. 2013

Biotechnology Progress

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The commercial value of Terfezia claveryi, an edible desert truffle with important gastronomic, nutritional, and antioxidant properties, has led to growing interest in its cultivation. The erratic and slow growth of T. claveryi mycelium in vitro represents an impairment to obtain mycorrhizal plants, and it makes necessary to find a new culture medium able to overcome these drawbacks. In this work, we analyze the effect of cyclodextrins (CDs) on the growth of T. claveryi mycelium. Different parameters, including colony diameter, growth rate, and colony fresh weight, were evaluated, both in the presence and absence of these encapsulant agents. The results obtained confirm the ability of CDs to stimulate the growth of T. claveryi mycelium when present in the culture medium. A similar effect was observed when CDs were added to the culture medium of Tuber melanosporum. Three natural (α-, β-, and γ) and two modified (hydroxypropil-β and methyl-β) CDs were assayed. The best results were obtained with β-cyclodextrin, but no improvement was observed with its chemically modified derivatives. CDs complex the different compounds present in the culture medium which impair mycelial growth.

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“…Modifications of the retention properties of molecules when different CD concentrations are added to the mobile phase were found to be related to the stoichiometry and stability of the inclusion complexes formed. Our papers analysed the encapsulation process of different bioactive compounds, determining the stoichiometry of the guest molecule/CD complexes, and the encapsulation constants, using fatty acids and stilbenes, among others, as guest molecules (López‐Nicolás et al , ; López‐Nicolás and García‐Carmona, ; Rodríguez‐Bonilla et al , ; Rodríguez‐Bonilla et al , ). In 2013 we used natural and modified CDs to study the encapsulation process of MeJA but it was not possible to separate the four stereoisomers (López‐Nicolás et al , ).…”

Section: Introductionmentioning

confidence: 99%

“…Recently our group has published several studies concerning the use of CDs as agents in the mobile phase of HPLC systems (López-Nicolás and García-Carmona, 2008;Rodríguez-Bonilla et al, 2010;Rodríguez-Bonilla et al, 2011). Modifications of the retention properties of molecules when different CD concentrations are added to the mobile phase were found to be related to the stoichiometry and stability of the inclusion complexes formed.…”

Section: Introductionmentioning

confidence: 99%

Separating and Identifying the Four Stereoisomers of Methyl Jasmonate by RP‐HPLC and using Cyclodextrins in a Novel Way

Matencio

Bermejo-Gimeno

García-Carmona

et al. 2016

Phytochemical Analysis

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Use of reversed phase high pressure liquid cromatography for the physicochemical and thermodynamic characterization of oxyresveratrol/β-cyclodextrin complexes

Cited by 29 publications

References 33 publications

Microemulsion-Based Oxyresveratrol for Topical Treatment of Herpes Simplex Virus (HSV) Infection: Physicochemical Properties and Efficacy in Cutaneous HSV-1 Infection in Mice

Microemulsion-Based Oxyresveratrol for Topical Treatment of Herpes Simplex Virus (HSV) Infection: Physicochemical Properties and Efficacy in Cutaneous HSV-1 Infection in Mice

Mycelium growth stimulation of the desert truffleTerfezia claveryichatin by β‐cyclodextrin

Separating and Identifying the Four Stereoisomers of Methyl Jasmonate by RP‐HPLC and using Cyclodextrins in a Novel Way

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