Use of RP4-prime plasmids constructed in vitro to promote a polarized transfer of the chromosome in Escherichia coli and Rhizobium meliloti

Julliot, J. S.; Boistard, P.

doi:10.1007/bf00268639

Cited by 45 publications

(25 citation statements)

References 28 publications

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“…Alternatively, the obligatory inheritance of ser-I+ could indicate an orientated mobilization of the P. morganii chromosome by either of these plasmids, with ser-l+ a proximal marker. However, with this mode of transfer, inheritance of proximal markers is not absolute (Coetzee, 1975;Juliot & Boistard, 1979). The finding that recombinants express the complete set of plasmid markers as well as the ser-lf marker is inconsistent with an Escherichia coli Hfr-mediated type transfer (Hayes, 1968) and is similar to that observed in S. coelicolor A3(2) NF x U F matings where all progeny express the NF donor property (Hopwood et aZ., 1969).…”

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confidence: 52%

Mobilization of the Proteus morganii Chromosome by R Plasmids

Beck

Coetzee

1980

Microbiology

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R plasmids R702., R711b, R1, D, Rip69, R447b, R471 andiR394, belonging to different incompatibility groups, mobilized the Proteus rnorganii 28 15 chromosome. Matings employing plasmids K711 b or R702 as sex factors with doubly auxotrophic recipients produced recombinants characterized by the obligatory inheritance of ser-I+, irrespective of the selected marker. I N T R O D U C T I O NProteus rnorganii occupies a unique position in the Proteus group. It differs in biochemical aspects as well as in its G + C mole percentage (see Coetzee, 1972). The chromosome of P. rnirabilis is mobilized by various R plasmids (Coetzee, 1978a(Coetzee, , b, 1979a) and a map of the chromosome is available (Coetzee, 1979 b). This paper reports attempts to mobilize the P. morganii chromosome with R plasmids. METHODSBacteria undplasmids. These are listed in Table 1. Media. Nutrient broth and agar were as described by Coetzee ( 1 9 7 8~) .MacConkey agar was from Difco. Minimal medium (MM) was that of Grabow & Smit (1967) containing methionine (4.0 pg ml-l) and calcium pantothenate (5 pg ml-l) and was supplemented with amino acids (40 pg ml-l) when necessary. Incubation temperature was 37 "C.Antibacterial drugs. Ampicillin, chloramphenicol, tetracycline and kanamycin (each 30 pg ml-l), nalidixic acid (75 ,ug ml-l) and streptomycin ( 1 mg ml-l) were added to MM or MacConkey agar when required.Plate matings. These were done by the method of Coetzee ( 1 9 7 8~) . Briefly, equal volumes (0.4 ml) of the partners were mixed and filtered through Millipore membranes which were then placed on nutrient agar and incubated overnight. Growth was harvested and washed with saline and suitable dilutions were plated on selective media and incubated for 4 d. Recombinants were tested for expression of plasmid antibiotic resistance markers by the method of Coetzee (1978a, 19796).Umelected marker analysis. Recombinants (50 to 500) selected for the transfer of a single marker on appropriately supplemented MM were replicated to MM to score prototrophs. R E S U L T S A N D D I S C U S S I O NPlasmids R702, R711b, R1, D, Rip69, R447b, R471 and R394 were stably maintained in P. morganii 28115, had intra-strain transfer frequencies of per donor (not shown) and mobilized the P. morganii chromosome. Plasmids Rip69, R447b, R471 or

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confidence: 52%

Mobilization of the Proteus morganii Chromosome by R Plasmids

Beck

Coetzee

1980

Microbiology

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“…Bacterial strains, plasmids, and phages are described in Table 1. Escherichia coli strains were grown on L broth (18). TY complete medium supplemented with 200 ,ug of rifampin (Rif) per ml (when selecting Rif' strains) or M9 mineral medium was used for R. meliloti (18,30).…”

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confidence: 99%

A new symbiotic cluster on the pSym megaplasmid of Rhizobium meliloti 2011 carries a functional fix gene repeat and a nod locus

Renalier¹,

Batut²,

Ghai³

et al. 1987

J Bacteriol

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A 290-kilobase (kb) region of the Rhizobium melloti 2011 pSym megaplasmid, which contains nodulation genes (nod) as well as genes involved in nitrogen fixation (nif andflx), was shown to carry at least six sequences repeated elsewhere in the genome. One of these reiterated sequences, about 5 kb in size, had previously been identified as part of a cluster offix genes located 220 kb downstream of the nifHDK promoter. Deletion of the reiterated part of this fix cluster does not alter the symbiotic phenotype. Deletion of the second copy of this reiterated sequence, which maps on pSym 40 kb upstream of the nijHDK promoter, also has no effect. Deletion

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“…DNA was probed with the following plasmids: pRWRm13 (Wheatcroft & Watson 1987), which contains 0.9 kb of the internal part of the R . meliloti insertion sequence ISRml ; pRmR2 (Ruvkun et al 1982), which contains a 3.9 kb segment DNA from R. meliloti carrying nifH and part of n i p ; pGMI42R (Julliot & Boistard 1979), which contains a 10 kb sequence of R. meliloti chromosomal DNA. Total, or plasmid DNA alone, was restricted with EcoRl and hybridized with the different probes (Hartmann 1989;Hartmann & Amarger 1991).…”

Section: N a S T U D I E Smentioning

confidence: 99%