Use of saturated sodium chloride solution as a tissue fixative

Al-Sarraj, Ayad A.

doi:10.33899/ijvs.2010.5582

Cited by 11 publications

(11 citation statements)

References 7 publications

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“…Therefore, the macroscopic aspect of brains at extraction is closer, in size and consistency, to that of a fresh brain. Additionally, previous studies using the same fixative have shown good cellular morphology in histology sections of the nervous tissue 27 . To our knowledge, specific antigens have not been assessed in nervous tissue fixed in this way.…”

Section: Fixative Solutionmentioning

confidence: 71%

“…deformation due to manipulation of the unfixed brain) and, at the same time, it has the potential of becoming a new source to obtain brain specimens for neuroscientific research projects. Our project takes advantage of the fixation method used by anatomists 15,17,27 , which by perfusion of the whole body allows the fixation of the brain in situ. Our anatomy lab performs all the perfusions within the first 48 hours post-mortem, ensuring a minimal decay of tissues prior to fixation.…”

Section: Discussionmentioning

confidence: 99%

“…Our anatomy lab employs different fixative solutions to fulfill specific aims (mainly saturated salt fixation for neurosurgical simulation purposes), so our pilot study aimed to test this solution that, based on our prior teaching experience, preserves the brain and meninges well in terms of volume and consistency. The salt saturated solution 26 is not standardly used in most anatomy labs, but has been shown to preserve cell morphology of various tissues well, including nervous tissue 26,27 .…”

Section: Discussionmentioning

confidence: 99%

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Proof of concept of a novelex vivo, in situmethod for MRI and histological brain assessment

Maranzano

Dadar

Bertrand-Grenier

et al. 2020

Preprint

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MRI-histology correlation studies of the ex vivo brain mostly employ fresh, extracted (ex situ) specimens, aldehyde fixed by immersion. This method entails manipulation of the fresh brain during extraction, introducing several disadvantages: deformation of the specimen prior to MRI acquisition; introduction of air bubbles in the sulci, creating artifacts; and uneven or poor fixation of the deeper regions of the brain.We propose a new paradigm to scan the ex vivo brain, exploiting a technique used by anatomists: fixation by whole body perfusion, which implies fixation of the brain in situ. This allows scanning the brain surrounded by fluids, meninges, and skull, thus preserving the structural relationships of the brain in vivo and avoiding the disadvantages of ex situ scanning. Our aims were: 1) to assess whether months of in situ fixation resulted in a loss of fluid around the brain; 2) to evaluate whether in situ fixation modified antigenicity for myelin and neuron specific marker; 3) to assess whether in situ fixation improved the register of ex vivo brain images to standard neuroanatomical templates in pseudo-Talairach space for morphometry studies.Five head specimens fixed with a saturated sodium chloride solution (a non-standard fixative used in our anatomy laboratory for neurosurgical simulation) were employed. We acquired 3D T1weighted (MPRAGE), 2D fluid-attenuated inversion recovery T2-weighted turbo spin echo (T2w-FLAIR), and 3D gradient-echo (3D-GRE) pulse sequences of all brains on a 1.5T MRI. After brain extraction, sections were processed for binding with myelin basic protein (MBP) and neuronal nuclei (NeuN) primary antibodies by immunofluorescence.This study showed that all but one specimen retained fluids in the subarachnoid and ventricular spaces. The specimen that lost fluid was the oldest one, with the longest interval between the time of death and the MRI scanning day being 403 days. All T1-weighted images were successfully processed through a validated pipeline used with in vivo MRIs. The pipeline did not require any modification to run on the ex vivo-in situ scans. All scans were successfully registered to the brain template, more accurately than an ex vivo-ex situ scan and exhibited positive antigenicity for MBP and NeuN.MRI and histology study of the ex vivo-in situ brain fixed by perfusion is feasible and allows for in situ MRI imaging for of at least 10 months post-mortem prior to histology analyses. Fluids around and inside the brain specimens and antigenicity for myelin and neurons were all well preserved.

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Section: Fixative Solutionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Proof of concept of a novelex vivo, in situmethod for MRI and histological brain assessment

Maranzano

Dadar

Bertrand-Grenier

et al. 2020

Preprint

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“…Al-Hubaity and Al-Saraj [10] had carried out work on saturated table salt and suggested that it could be used in place of formalin to preserve bodies [10]. Al-Saraj [1] worked on saturated sodium chloride solution and concluded that it can be used as a fixative in histopathological procedures. The results obtained were same as that obtained by using conventional formaldehyde.…”

Section: Discussionmentioning

confidence: 99%

“…Fixation should also provide for the preservation of tissue substances and proteins. Therefore, it is regarded the first step and the foundation in a sequence of events that culminates in the final examination of a tissue section [1].…”

Section: Introductionmentioning

confidence: 99%

Artefacts Produced by Normal Saline When Used as a Holding Solution for Biopsy Tissues in Transit

Sengupta¹,

Prabhat²,

Gupta³

et al. 2013

J. Maxillofac. Oral Surg.

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Oral surgeons, surgeons and dentists carrying out small biopsy procedures on an out-patient basis have been frequently sending poorly stored biopsy tissues to the Departments of Oral Pathology of various dental colleges, wherein the tissues were being stored in normal saline and then transferred to formalin. Such tissues often show abnormal cellular and architectural changes, which pose a challenge to diagnosis, especially when the histopathologist is not aware that the tissue was stored in normal saline for some time. We wanted to know the extent of artefactually induced cellular and architectural changes in tissues that were transported in normal saline and received by us, based on the duration for which they were held in normal saline. Normal oral soft tissue was subjected to storage in normal saline for periods varying from 1 to 36 h, before being fixed in 10 % formalin. Soft tissue specimens were collected during routine surgical extractions and each was cut into six parts. One was transferred immediately to formalin as control and other bits kept in normal saline for intervals of 1, 3, 12, 24, and 36 h before fixing in formalin. A comparative photographic analysis was carried out to analyze the histopathological changes. The tissues kept in normal saline before fixation underwent various changes. In no way should normal saline be used, even for a short time, to store and transport excised tissue; it is imperative that such tissues be placed immediately in a proper fixative like 10 % formalin.

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The Usefulness of Saturated Salt Solution Embalming Method for Oral Surgical Skills Training: A New Cadaveric Training Model for Bone Harvesting

Watanabe

Yoneyama

Hamada

et al. 2019

Anatomical Sciences Ed

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The purpose of this study was to assess the usefulness of saturated salt solution‐embalmed cadavers for oral surgical skills training related to bone graft harvesting. Two half‐day surgical skills training workshops were held at the Tokyo Medical University utilizing eight cadavers embalmed with the saturated salt solution. A total of 22 participants including oral surgeons, residents, and dentists attended the workshop. Surgical training consisted of six procedures related to intraoral and extraoral bone harvesting. The participants were surveyed to assess self‐confidence levels for each surgical procedure before and after completion of each workshop. The Wilcoxon signed‐rank test was used to compare the differences between each median score before and after the workshop. There were statistically significant increases in the self‐assessed confidence scores in bone harvesting procedures for the zygomatic bone (P = 0.003), maxillary tuberosity (P = 0.002), and other sites (P < 0.001). The anatomical features of saturated salt solution‐embalmed cadavers were also examined. The textures of the oral mucosa and skin were similar to those of living individuals. The structure of bone tissues was well‐preserved and the hardness was realistic. Consequently, all procedures were performed with sufficient realism. The saturated salt solution method has a relatively low cost of preparation and storage, and almost no odor. The authors suggest that saturated salt solution‐embalmed cadavers could provide a new model for oral surgical skills training in bone harvesting.

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Use of saturated sodium chloride solution as a tissue fixative

Cited by 11 publications

References 7 publications

Proof of concept of a novelex vivo, in situmethod for MRI and histological brain assessment

Proof of concept of a novelex vivo, in situmethod for MRI and histological brain assessment

Artefacts Produced by Normal Saline When Used as a Holding Solution for Biopsy Tissues in Transit

The Usefulness of Saturated Salt Solution Embalming Method for Oral Surgical Skills Training: A New Cadaveric Training Model for Bone Harvesting

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