Use of sequence analysis of the VP4 gene to classify recent Vietnamese rotavirus isolates

Nguyen, Tuan Anh; Hoang, Le Phuc; Pham, Le Duc; Hoang, Kim Trong; Okitsu, Shoko; Mizuguchi, Masashi; Ushijima, Hiroshi

doi:10.1111/j.1469-0691.2007.01918.x

Cited by 22 publications

(15 citation statements)

References 22 publications

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“…In this study, the overall incidence of P[8]b in RVA‐positive samples was 0.8%, but it was 2.7% in the 2007–2008 season (Table I). These incidences are consistent with those (0.9–4.4%) reported by other investigators [Cunliffe et al, 2001; Nguyen et al, 2008; Paul et al, 2008; Samajdar et al, 2008; Nagashima et al, 2010]. Of note, Japanese P[8]b strains were detected only in May 2008, and both patients infected with P[8]b strains lived in the same city, suggesting that the prevalence of P[8]b RVAs in Okayama Prefecture is limited considerably.…”

Section: Disribution Of G and P Genotype Combination Of Rva Strains Isupporting

confidence: 93%

“…The OP354 strain has a genetically distinct P[8], which was reported first in Malawi during the 1998–1999 season [Cunliffe et al, 2001]. OP354‐like P[8] strains were detected recently in other countries, including India [Samajdar et al, 2008], Bangladesh [Nagashima et al, 2009], Thailand [Theamboonlers et al, 2008], Vietnam [Nguyen et al, 2008; Tamura et al, 2010], and Finland, suggesting a global distribution of these viruses. Nagashima et al [2009] determined recently full‐length VP4 gene sequences of the two OP354‐like P[8] strains, MMC38 and MMC71, which were isolated in Bangladesh.…”

Section: Disribution Of G and P Genotype Combination Of Rva Strains Imentioning

confidence: 99%

“…This is the first report of P[8]b RVA isolation in Japan to date. The prototype P[8]b RVA (strain OP354) was identified in Malawi during the 1998–1999 season [Cunliffe et al, 2001], before subsequent P[8]b RVAs were detected mainly in Asian countries [Nguyen et al, 2008; Samajdar et al, 2008; Theamboonlers et al, 2008; Nagashima et al, 2009; Tamura et al, 2010], but not in Japan. Using the conventional RT‐PCR method for P genotyping, multiple faint bands were observed with strains OH1998 and OH2024; therefore, these presumptively were determined to be a mixed genotype of P[4] and P[8].…”

Section: Disribution Of G and P Genotype Combination Of Rva Strains Imentioning

confidence: 99%

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Molecular characterization of OP354‐like P[8] (P[8]b subtype) human rotaviruses a species isolated in Japan

Kuzuya¹,

Fujii²,

Hamano³

et al. 2012

Journal of Medical Virology

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OP354-like P[8] (P[8]b subtype) species A rotaviruses (RVAs) were isolated first time in Japan during a RVA survey in Okayama Prefecture between 2006 and 2009. Two of 236 RVA-positive samples were identified as G1P[8]b by reverse transcription polymerase chain reaction. P[8]b strains (RVA/human-wt/JPN/OH1998/2008/G1P[8]b and RVA/human-wt/JPN/OH2024/2008/G1P[8]b) were isolated only in May, 2008 and both patients infected with P[8]b viruses lived in the same city, suggesting that the prevalence of P[8]b RVAs is limited considerably in Okayama Prefecture. Molecular analysis of four genes (VP4, VP6, VP7, and NSP4 genes) of Japanese P[8]b strains revealed that the VP4 genes of these strains were related closely to those of Southeast Asian and Indian P[8]b strains. In contrast, the VP6, VP7, and NSP4 genes of Japanese P[8]b strains were highly homologous to G1P[8]a strains prevalent in the same area. These results suggest that the Japanese P[8]b strain may be a result of reassortment events between Japanese G1P[8]a viruses and unidentified Asian viruses possessing the P[8]b VP4 gene.

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Section: Disribution Of G and P Genotype Combination Of Rva Strains Isupporting

confidence: 93%

Section: Disribution Of G and P Genotype Combination Of Rva Strains Imentioning

confidence: 99%

Section: Disribution Of G and P Genotype Combination Of Rva Strains Imentioning

confidence: 99%

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Molecular characterization of OP354‐like P[8] (P[8]b subtype) human rotaviruses a species isolated in Japan

Kuzuya¹,

Fujii²,

Hamano³

et al. 2012

Journal of Medical Virology

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“…BE1280 clustered closely with previously characterized RVA strains from Bangladesh (39) and showed a remarkable amount of differences in antigenic epitopes with other P [8] lineages. OP354-like P [8] strains are increasingly reported and have been found in combination with various G genotypes throughout Eurasia and Africa (11,25,39,40,52). Only limited research on the antigenic properties of OP354-like strains has been conducted to date, but neutralization plaque assays performed with antibodies directed against VP5 ‫ء‬ indicate that the antigenic properties of OP354-like strains appear to be similar as P [8] lineage 3 strains (39).…”

Section: Discussionmentioning

confidence: 99%

Genetic Analyses Reveal Differences in the VP7 and VP4 Antigenic Epitopes between Human Rotaviruses Circulating in Belgium and Rotaviruses in Rotarix and RotaTeq

et al. 2012

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Group A rotaviruses (RVAs) are a primary cause of gastroenteritis in children under 5 years of age and are associated with over 500,000 deaths annually, of which the majority occur in developing countries (42, 43). RVAs belong to the family Reoviridae, and the infectious RVA virion is a triple-layered icosahedral particle that contains 11 segments of double-stranded RNA (18). The outer capsid layer is composed of the spike protease-sensitive attachment protein VP4 (P) and the glycoprotein VP7 (G). The nucleotide sequences of the VP7-and VP4-encoding segments form the basis of a dual classification system that defines the G and P genotypes of RVAs, respectively. Although 27 G genotypes and 35 P genotypes have been identified to date (31), only a few RVA G and P genotype combinations contribute substantially to the burden of human disease (29,30,32,53 (33,35). An increase in prevalence of G12 RVAs, mainly associated with P[8] or P [6] and to a lesser extent with P[4] or P[9] VP4s, was observed around the turn of the century. The G12 RVA strains are now considered the sixth common global genotype (29,33,35,49).Two live attenuated oral RVA vaccines, Rotarix (GlaxoSmithKline Biologicals, Belgium) and RotaTeq (Merck & Co., Inc., United States), have been licensed for use in many countries around the world. Rotarix is derived from the attenuated human G1P[8] RVA strain 89-12, which was isolated in Cincinnati, OH, in 1988 (65). RotaTeq contains five human-bovine reassortant RVA strains (WI79-9, SC2-9, WI78-9, BrB-9, and WI79-4, referred to as G1, G2, G3, G4, and P1 reassortants, respectively, for simplicity). The G1 to G4 reassortants each express one of the VP7 proteins of the human RVA parental strains WI79 (G1), SC2 (G2), WI78 (G3), and BrB (G4) and the VP4 protein of the bovine RVA strain WC3 (P7[5]), whereas the P1 reassortant expresses the VP4 protein of the human RVA strain WI79 (P1A [8]) and the VP7 protein of the bovine RVA strain WC3 (G6) (9, 34). Human RVA SC-2 was isolated in 1981 at St. Christopher's Hospital of Philadelphia, and the WI79 and WI78 RVAs were isolated in 1983 at the Children's Hospital of Philadelphia, while the BrB (originally Bricout B) RVA strain was isolated in 1984 at L'Hôpital Armand Trousseau (Paris, France) (34). Both RVA vaccines have been proven to be safe and efficacious in large-scale clinical trials (9,51,59,61,62). The mechanisms by which the vaccines induce immunologic protection in infants have not been clearly elucidated. It likely includes the induction of serum and intestinal serotypespecific neutralizing antibodies directed against VP7 and VP4 and virus-specific cytotoxic T lymphocytes (20,63,64). However, proteins other than VP7 and VP4 may be involved in immune protection as well.

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“…Enteric viruses are a predominant cause of acute childhood gastroenteritis in Vietnam 9,11,20,21,30–33. Our study was designed to examine the prevalence and distribution of four enteric viruses causing hospitalization in children attending two defined healthcare centers in one rural and one urban location in southern Vietnam.…”

Section: Discussionmentioning

confidence: 99%

The Emergence of Rotavirus G12 and the Prevalence of Enteric Viruses in Hospitalized Pediatric Diarrheal Patients in Southern Vietnam

My¹,

Rabaa²,

Vinh³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Diarrhea is a major cause of childhood morbidity and mortality in developing countries, and the majority of infections are of viral etiology. We aimed to compare the etiological prevalence of the major enteric viruses in an urban and a rural setting in southern Vietnam. We simultaneously screened fecal specimens from 362 children in Ho Chi Minh City and Dong Thap province that were hospitalized with acute diarrhea over a 1-month-long period for four viral gastrointestinal pathogens. Rotavirus was the most common pathogen identified, but there was a differential prevalence of rotavirus and norovirus between the urban and rural locations. Furthermore, rotavirus genotyping and phylogenetic analysis again differentiated the genotypes by the sampling location. Our data show a disproportional distribution of enteric viral pathogens in urban and rural locations, and we provide evidence of continual importation of new rotavirus strains into southern Vietnam and report the emergence of rotavirus genotype G12.

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Use of sequence analysis of the VP4 gene to classify recent Vietnamese rotavirus isolates

Cited by 22 publications

References 22 publications

Molecular characterization of OP354‐like P[8] (P[8]b subtype) human rotaviruses a species isolated in Japan

Molecular characterization of OP354‐like P[8] (P[8]b subtype) human rotaviruses a species isolated in Japan

Genetic Analyses Reveal Differences in the VP7 and VP4 Antigenic Epitopes between Human Rotaviruses Circulating in Belgium and Rotaviruses in Rotarix and RotaTeq

The Emergence of Rotavirus G12 and the Prevalence of Enteric Viruses in Hospitalized Pediatric Diarrheal Patients in Southern Vietnam

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