Use of Size and a Copolymer Design Feature To Improve the Biodistribution and the Enhanced Permeability and Retention Effect of Doxorubicin-Loaded Mesoporous Silica Nanoparticles in a Murine Xenograft Tumor Model

Meng, Huan; Xue, Min; Xia, Tian; Ji, Zhaoxia; Tarn, Derrick; Zink, Jeffrey I.; Nel, André E.

doi:10.1021/nn200809t

Cited by 464 publications

(401 citation statements)

References 63 publications

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“…Very recently, several studies have demonstrated that SN particles not only can serve as promising drug carriers for high tumor targeting efficiency and therapy efficacy, but also for reducing the systematic toxicity [16,17]. However, the biological safety effect is sill being a pendent question of SN particles [18,19].…”

Section: Discussionmentioning

confidence: 99%

Acute toxicity and oxidative damage induced by silica nanorattle in vivo

Liu

Tang

et al. 2012

Chin. Sci. Bull.

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Hollow mesoporous nanomaterial is a kind of promising new drug delivery system due to their unique hollow structures. In order to evaluate the toxicity of silica nanorattle (SN) particles in vivo, 40 female mice were used in this study to investigate the acute toxicity and oxidative damage. Mice were intravenously injected with SN suspension in sterile 5% glucose at 40, 80 and 240 mg/kg, respectively. The control group was administrated with equal-volume 5% glucose. Weight, feed intake, hematology analysis, blood biochemical assay and histopathology diagnosis were examined. The activities of SOD, GSH and CAT were measured as well. The results demonstrated that the levels of ALT and AST in the mice treated with 240 mg/kg SN increased significantly as compared with the control group (P<0.05), whereas the contents of BUN and CREA changed unremarkably. The activity of SOD induced by SN in the liver decreased significantly (P<0.05). In summary, this study revealed that liver was the target organ of the SN. It also can be concluded that activity of SOD played an important role in liver injury caused by SN. silica nanorattle, acute toxicity, oxidative stress, superoxide dismutase Citation:Fu C H, Liu T L, Tang F Q, et al. Acute toxicity and oxidative damage induced by silica nanorattle in vivo.

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Section: Discussionmentioning

confidence: 99%

Acute toxicity and oxidative damage induced by silica nanorattle in vivo

Liu

Tang

et al. 2012

Chin. Sci. Bull.

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“…Hemolysis assay in vitro was employed to evaluate toxicity of LDMP copolymer (Cerda-Cristerna et al, 2011;Meng et al, 2011). Heparinized rat erythrocytes were separated from rat blood by centrifugation at 3000 r/min for 30 min in a centrifuge and eluted using physiological saline to achromatic color for supernatant solution.…”

Section: Hemolysis Test Of Copolymersmentioning

confidence: 99%

Linear-dendrimer type methoxy-poly (ethylene glycol)-b-poly (ɛ-caprolactone) copolymer micelles for the delivery of curcumin

et al. 2014

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(2015) Linear-dendrimer type methoxy-poly (ethylene glycol)-b-poly (ɛ-caprolactone) copolymer micelles for the delivery of curcumin, Drug Delivery, 22:1, 58-68, DOI: 10.3109/10717544.2014 Methods: A novel linear-dendrimer methoxy-poly (ethylene glycol)-b-poly ("-caprolactone) copolymer was synthesized through O-alkylation, basic hydrolysis and ring-opening polymerization reaction with methoxy-poly (ethylene glycol), epichlorohydrin and "-caprolactone as raw materials. Its structure was characterized by 1 H-NMR and GPC. The copolymer's hemolysis and micellar encapsulation for curcumin by thin-film hydration were studied. Curcumin-loaded micelles were evaluated by use of in vitro release, FT-IR and X-ray diffraction. Curcumin-loaded micelles' in vitro cytotoxic activities against Hela and HT-29 cells were done, and its pharmacokinetic parameters were also carried out. Results: Curcumin was encapsulated into the micelles with 92.54% of entrapment efficiency and 12.84% of drug loading in amorphous forms. The dissolubility of nanoparticulate curcumin was 1.70 Â 10 5 times higher than that of curcumin in water. The obtained copolymer showed no hemolysis. In vitro drug release study indicated that, in all cases, the kinetics was adjusted well to the Makoid-Banakar model (R 2 abj ¼ 0.9984). In addition, data were analyzed by the Korsmeyer-Peppas model, n values were 0.43, indicating that the drug release was accomplished by the combination diffusion and polymer chain relaxation. The cytotoxicity experiment indicated that the nanoparticulate curcumin kept up its potent anti-cancer activities. The pharmacokinetic results showed that the MRT 0-1 , t 1/2z and AUC 0-1 of Curcuminloaded micelles were 1.64, 6.54 and 4.67 times higher than that of CUR control solution. Conclusions: The copolymeric micelles loading curcumin might act as a delivery vehicle for CUR.

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“…27,28 SiO 2 -NH 2 -coated SPIO (SPIO@SiO 2 -NH 2 ) nanoparticles prepared by alkaline hydrolysis of APTES have been reported by the present authors 29 and other groups. [30][31][32] In a previous study, SPIO@SiO 2 -NH 2 nanoparticles were applied to rabbit mesenchymal stem cell (MSC) labeling. 29 It was shown that SPIO@ SiO 2 -NH 2 nanoparticles had a higher labeling efficiency than SiO 2 -coated SPIO (SPIO@SiO 2 ) nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Enhanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines

Zhu¹,

Wáng²,

Leung³

et al. 2012

IJN

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Purpose:To compare the cellular uptake efficiency and cytotoxicity of aminosilane (SiO 2 -NH 2 )-coated superparamagnetic iron oxide (SPIO@SiO 2 -NH 2 ) nanoparticles with three other types of SPIO nanoparticles coated with SiO 2 (SPIO@SiO 2 ), dextran (SPIO@dextran), or bare SPIO in mammalian cell lines. Materials and methods: Four types of monodispersed SPIO nanoparticles with a SPIO core size of 7 nm and an overall size in a range of 7-15 nm were synthesized. The mammalian cell lines of MCF-7, MDA-MB-231, HT-29, RAW264.7, L929, HepG2, PC-3, U-87 MG, and mouse mesenchymal stem cells (MSCs) were incubated with four types of SPIO nanoparticles for 24 hours in the serum-free culture medium Dulbecco's modified Eagle's medium (DMEM) with 4.5 µg/mL iron concentration. The cellular uptake efficiencies of SPIO nanoparticles were compared by Prussian blue staining and intracellular iron quantification. In vitro magnetic resonance imaging of MSC pellets after SPIO labeling was performed at 3 T. The effect of each SPIO nanoparticle on the cell viability of RAW 264.7 (mouse monocyte/macrophage) cells was also evaluated. Results: Transmission electron microscopy demonstrated surface coating with SiO 2 -NH 2 , SiO 2 , and dextran prevented SPIO nanoparticle aggregation in DMEM culture medium. MCF-7, MDA-MB-231, and HT-29 cells failed to show notable iron uptake. For all the remaining six cell lines, Prussian blue staining and intracellular iron quantification demonstrated that SPIO@ SiO 2 -NH 2 nanoparticles had the highest cellular uptake efficiency. SPIO@SiO 2 -NH 2 , bare SPIO, and SPIO@dextran nanoparticles did not affect RAW 264.7 cell viability up to 200 µg Fe/mL, while SPIO@SiO 2 reduced RAW 264.7 cell viability from 10 to 200 µg Fe/mL in a dose-dependent manner. Conclusion: Cellular uptake efficiency of SPIO nanoparticles depends on both the cell type and SPIO surface characteristics. Aminosilane surface coating enhanced the cellular uptake efficiency without inducing cytotoxicity in a number of cell lines.

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Use of Size and a Copolymer Design Feature To Improve the Biodistribution and the Enhanced Permeability and Retention Effect of Doxorubicin-Loaded Mesoporous Silica Nanoparticles in a Murine Xenograft Tumor Model

Cited by 464 publications

References 63 publications

Acute toxicity and oxidative damage induced by silica nanorattle in vivo

Acute toxicity and oxidative damage induced by silica nanorattle in vivo

Linear-dendrimer type methoxy-poly (ethylene glycol)-b-poly (ɛ-caprolactone) copolymer micelles for the delivery of curcumin

Enhanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines

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