Use of sodA sequencing for the identification of clinical isolates of coagulase-negative staphylococci

Abstract

This study evaluated the possible advantages provided by a genotypic method over commercially available biochemical systems for the identification of clinical isolates of coagulase-negative staphylococci (CNS). Partial sequencing of the sodA gene was performed for 168 coagulase-negative clinical isolates of staphylococci identified previously with the ID32 STAPH system. Of these, 101 (60.1%) were identified to the species level with ID32 STAPH, while 67 (39.9%) were misidentified or not identified with certainty. Sequencing of sodA proved useful for resolving all ambiguities or inconclusive identifications generated by the commercially available biochemical identification system.

Investigation of Glycopeptide Susceptibility of Coagulase-Negative Staphylococci (CoNS) From a Tertiary Care Hospital in Gorgan, Northern Iran

“…The current study revealed that S. haemolyticus and S. epidermidis were the most prevalent CoNS from clinical isolates. This result, in line with many previous studies, confirms the predominance of these two species in various clinical samples compared to other CoNS species (28)(29)(30)(31). In addition, S. haemolyticus and S. epidermidis have been reported to be among the most frequent CoNS species acquiring resistance to multiple antibiotics, which is in agreement with the results of the current study (3,8).…”

Genetic Analysis of Glycopeptide-Resistant Staphylococcus epidermidis Strains from Bone and Joint Infections

“…All isolates were identified to the species level by partial sodA sequencing (23). A disk diffusion method was used for antibiotic susceptibility testing according to the EUCAST 2012 recommendations (http://www.eucast.org/antimicrobial_susceptibility_testing/); strains with inconclusive results for resistance to methicillin were further studied by testing for the mecA gene (24).…”

Decreased Susceptibility to Teicoplanin and Vancomycin in Coagulase-Negative Staphylococci Isolated from Orthopedic-Device-Associated Infections

“…The "CoNS group" was identified using Gram staining and the following tests: catalase, Slidex latex agglutination (bioMérieux), and tube coagulase (Bio-Rad, Marnes la Coquette, France). CoNS isolates were then identified to the species level by partial sodA sequencing, as described elsewhere (17). The susceptibilities of strains to 15 antibiotics were determined by the disk diffusion method on Mueller-Hinton agar (Bio-Rad) (4) using the following disks: penicillin (6 g), oxacillin (5 g), ofloxacin (5 g), erythromycin (15 g), lincomycin (15 g), pristinamycin (15 g), rifampin (30 g), gentamicin (10 IU), kanamycin (30 g), tobramycin (10 g), tetracycline (30 g), trimethoprim-sulfamethoxazole (1.25 and 23.75 g, respectively), fosfomycin (50 g), vancomycin (30 g), and teicoplanin (30 g).…”