Use of solid phase extraction for the isolation and clean-up of a derivatised furazolidone metabolite from animal tissues

Conneely, A.; Nugent, A. M.; O’Keeffe, Michael

doi:10.1039/b203058f

Cited by 47 publications

(40 citation statements)

References 6 publications

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“…Tissue bound nitrofuran metabolites are small molecular species which are usually derivatised using ortho-nitrobenzaldehyde (o-NBA) in order to increase molecular mass and improve the sensitivity of detection (Conneely et al, 2002). Prior to derivatisation, the release of bound metabolites from tissue is carried out under mildly acidic conditions (Figure 2b).…”

Section: Sample Preparation Methodsmentioning

confidence: 99%

“…Szilagyi and de la Calle (2006) detected semicarbazide in egg tissue using solid phase extraction in conjunction with LC-MS/MS . Conneely et al (2002) on the other hand, implemented additional clean up measures for the detection of AOZ in pig liver. In this study, a protease digestion step was introduced to overcome matrix effects and allowed the simple application of filtered supernatants to SPE columns.…”

Section: Lc-ms/ms Conditionsmentioning

confidence: 99%

“…In this study, a protease digestion step was introduced to overcome matrix effects and allowed the simple application of filtered supernatants to SPE columns. In an attempt to remove the excess o-NBA from the sample, the use of two different SPE cartridges was implemented resulting in the removal of 99% of the excess o-NBA (Conneely et al, 2002).…”

Section: Lc-ms/ms Conditionsmentioning

confidence: 99%

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Nitrofuran antibiotics: a review on the application, prohibition and residual analysis

Vass¹,

Hruška²,

Fránek³

2008

Vet. Med.

238

130

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Nitrofuran antibiotics, employed for the treatment of bacterial diseases in livestock production, were banned from use in the European Union (EU) in 1995 due to concerns about the carcinogenicity of their residues in edible tissue. This review provides an overview of nitrofuran toxicity, metabolism, and also specific aspects of legislation surrounding their prohibition. Special attention is devoted to semicarbazide -a nitrofuran metabolite and food contaminant. Analytical procedures for nitrofuran analysis in various matrices and validation requirements for screening and confirmation methods with respect to EU regulations are also reviewed.Keywords: nitrofurans; tissue bound metabolites; semicarbazide; bioavailability; mutagenicity; legislation; sample preparation; validation; detection methods List of abbreviations AHD = 1-aminohydantoin; AOZ = 3-amino-2-oxazolidinone; AMOZ = 3-amino-5-morpholino-methyl-1,3-oxazolidinone; CC α = decision limit; CC β = detection capability; EC = European Commission; EFSA = European Food Safety Authority; ELISA = enzyme linked immuno-adsorbent assay; ESI = electro-spray ionisation; EU = European Union; FTD = furaltadone; FZD = furazolidone; HPLC = high performance liquid chromatography; IC = inhibition concentration; LC = liquid chromatography; LOD = limit of detection; MS = mass spectrometry; NFT = nitrofurantoin; NFZ = nitrofurazone; NP = nitrophenyl; NPAHD = 3-(2-nitrobenzylidenamino)-2,4-imidazolidinedione); NPAMOZ = 5-(morpholinomethyl)-3-(2-nitrobenzylidenamino)-2-oxazolidinone); NPAOZ = [3-(2-nitrobenzylidenamino)-2-oxazolidinone]; NPSEM = 3[(2-nitrophenyl)methylene]-hydrazinecarboxamide; o-NBA = ortho-nitrobenzaldehyde; RASFF = Rapid Alert System for Food and Feed; SE = solvent extraction; SEM = semicarbazide; SPE = solid phase extraction; UV = ultraviolet

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Section: Sample Preparation Methodsmentioning

confidence: 99%

Section: Lc-ms/ms Conditionsmentioning

confidence: 99%

Section: Lc-ms/ms Conditionsmentioning

confidence: 99%

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Nitrofuran antibiotics: a review on the application, prohibition and residual analysis

Vass¹,

Hruška²,

Fránek³

2008

Vet. Med.

238

130

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“…As nitrofuran metabolites are covalently bound to proteins and are released from the tissues under slightly acidic conditions, they are derivatised with onitrobenzaldehyde (o-NBA) to form nitrophenyl (NP) derivatives prior to detection [16].…”

Section: Introductionmentioning

confidence: 99%

Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ)

Shen

Sun

et al. 2013

Talanta

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Publisher rights This is the author's version of a work that was accepted for publication in Talanta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Talanta, VOL 103, 01/2012 General rights Copyright for the publications made accessible via the Queen's University Belfast Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Cite this article as: Zhen-Lin Xu, Yu-Dong Shen, Yuan-Ming Sun, Katrina Campbell, Yuan-Xin Tian, Shi-Wei Zhang, Hong-Tao Lei and Yue-Ming Jiang, Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), Talanta, http://dx.doi.org/10. 1016/j.talanta.2012.10.059 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractA heterologous competitive indirect enzyme-linked immunosorbent assay (ciELISA) for the determination of the furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) was developed. AMOZ was derivatised with 2-(4-formylphenoxy)acetic acid or 2-(3-formylphenoxy)acetic acid to obtain two novel immunizing haptens. The ability of these haptens in producing specific polyclonal antibodies against the nitrophenyl derivative of AMOZ (NPAMOZ) was compared with that of traditional immunizing haptens (derivatised AMOZ with 3-carboxybenzaldehyle or 4-carboxybenzaldehyle). The results indicated that the novel immunizing haptens were able to produce antibodies with almost a two-fold improvement in sensitivity of the ciELISA for NPAMOZ in comparison with the existing antibody based ELISAs. The differences in sensitivity were explained by the molecular modeling of the lowest energy conformations of NPAMOZ and the haptens. Another novel hapten, derivatised AMOZ with 2-oxoacetic acid, was synthesized and used as a heterologous coating hapten. The results showed that this strategy of using only a partial structure of the target molecule as the coating hapten was able to obtain a two to three-fold improvement in sensitivity. This study provided a modern approach for the development of an immunoassay with improved sensitivity for the metabolites of nitrofuran antibiotics.

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“…These methods have been widely used in the extraction of metabolites from animal and plant tissues (Shryock et al, 1986;Kopka et al, 1995;Chen et al, 1998;Adams et al, 1999;Bouchereau et al, 2000;Conneely et al, 2002) and microorganisms (Cordeiro and Freire, 1996;Weuster-Botz, 1997;Hajjaj et al, 1998;Schaefer et al, 1999;Vaseghi et al, 1999;Letisse and Lindley, 2000;Martins et al, 2001;Buchholz et al, 2002;Buziol et al, 2002;Maharjan and Ferenci, 2003). Acidic extraction is commonly used for the extraction of nucleotides and water-soluble metabolites (Vaseghi et al, 1999;Martins et al, 2001;Buchholz et al, 2002), but for metabolome studies it is evident that not all metabolites are stable at the extreme pH, and destruction of some nucleotides has been reported during acidic extractions (Hajjaj et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Global metabolite analysis of yeast: evaluation of sample preparation methods

Villas‐Bôas

Højer-Pedersen

Åkesson

et al. 2005

Yeast

366

363

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Sample preparation is considered one of the limiting steps in microbial metabolome analysis. Eukaryotes and prokaryotes behave very differently during the several steps of classical sample preparation methods for analysis of metabolites. Even within the eukaryote kingdom there is a vast diversity of cell structures that make it imprudent to blindly adopt protocols that were designed for a specific group of microorganisms. We have therefore reviewed and evaluated the whole sample preparation procedures for analysis of yeast metabolites. Our focus has been on the current needs in metabolome analysis, which is the analysis of a large number of metabolites with very diverse chemical and physical properties. This work reports the leakage of intracellular metabolites observed during quenching yeast cells with cold methanol solution, the efficacy of six different methods for the extraction of intracellular metabolites, and the losses noticed during sample concentration by lyophilization and solvent evaporation. A more reliable procedure is suggested for quenching yeast cells with cold methanol solution, followed by extraction of intracellular metabolites by pure methanol. The method can be combined with reduced pressure solvent evaporation and therefore represents an attractive sample preparation procedure for high-throughput metabolome analysis of yeasts.

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Use of solid phase extraction for the isolation and clean-up of a derivatised furazolidone metabolite from animal tissues

Cited by 47 publications

References 6 publications

Nitrofuran antibiotics: a review on the application, prohibition and residual analysis

Nitrofuran antibiotics: a review on the application, prohibition and residual analysis

Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ)

Global metabolite analysis of yeast: evaluation of sample preparation methods

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