Use of some novel alternative electron sinks to inhibit ruminal methanogenesis

Ungerfeld, Emilio M.; Rust, S. R.; Burnett, Robert

doi:10.1051/rnd:2003016

Cited by 55 publications

(57 citation statements)

References 28 publications

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“…Finally, ethanol concentrations increased with NIT supporting the hypothesis that this alcohol may have a role in disposing reducing equivalents spared from methanogenesis (Ungerfeld et al, 2003). Overall, NIT stimulated [2H] producing pathways as shown by the greater acetate molar percentage.…”

Section: Discussionsupporting

confidence: 62%

Redirection of Metabolic Hydrogen by Inhibiting Methanogenesis in the Rumen Simulation Technique (RUSITEC)

2017

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A decrease in methanogenesis is expected to improve ruminant performance by allocating rumen metabolic hydrogen ([2H]) to more energy-rendering fermentation pathways for the animal. However, decreases in methane (CH4) emissions of up to 30% are not always linked with greater performance. Therefore, the aim of this study was to understand the fate of [2H] when CH4 production in the rumen is inhibited by known methanogenesis inhibitors (nitrate, NIT; 3-nitrooxypropanol, NOP; anthraquinone, AQ) in comparison with a control treatment (CON) with the Rumen Simulation Technique (RUSITEC). Measurements started after 1 week adaptation. Substrate disappearance was not modified by methanogenesis inhibitors. Nitrate mostly seemed to decrease [2H] availability by acting as an electron acceptor competing with methanogenesis. As a consequence, NIT decreased CH4 production (−75%), dissolved dihydrogen (H2) concentration (−30%) and the percentages of reduced volatile fatty acids (butyrate, isobutyrate, valerate, isovalerate, caproate and heptanoate) except propionate, but increased acetate molar percentage, ethanol concentration and the efficiency of microbial nitrogen synthesis (+14%) without affecting gaseous H2. Nitrooxypropanol decreased methanogenesis (−75%) while increasing both gaseous and dissolved H2 concentrations (+81% and +24%, respectively). Moreover, NOP decreased acetate and isovalerate molar percentages and increased butyrate, valerate, caproate and heptanoate molar percentages as well as n-propanol and ammonium concentrations. Methanogenesis inhibition with AQ (−26%) was associated with higher gaseous H2 production (+70%) but lower dissolved H2 concentration (−76%), evidencing a lack of relationship between the two H2 forms. Anthraquinone increased ammonium concentration, caproate and heptanoate molar percentages but decreased acetate and isobutyrate molar percentages, total microbial nitrogen production and efficiency of microbial protein synthesis (−16%). Overall, NOP and AQ increased the amount of reduced volatile fatty acids, but part of [2H] spared from methanogenesis was lost as gaseous H2. Finally, [2H] recovery was similar among CON, NOP and AQ but was largely lower than 100%. Consequently, further studies are required to discover other so far unidentified [2H] sinks for a better understanding of the metabolic pathways involved in [2H] production and utilization.

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Section: Discussionsupporting

confidence: 62%

Redirection of Metabolic Hydrogen by Inhibiting Methanogenesis in the Rumen Simulation Technique (RUSITEC)

2017

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“…It is presumably that the methane-suppressing effects of TS may lead to accumulations of hydrogen (Hu et al, 2005). Another way of diverting hydrogen away from methane formation would be to promote alternative electron-sink metabolic pathways to dispose of the reducing power (Ungerfeld et al, 2003), and fumarate has been shown to compete successfully for hydrogen (Newbold et al, 2005). However, further addition of DF did not have better inhibitory effects on methane emissions compared to TS addition only.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of methanogenesis by tea saponin and tea saponin plus disodium fumarate in sheep

Zhang¹,

Zhang²,

Zhou³

et al. 2007

J. Anim. Feed Sci.

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The study was conducted to investigate the effects of tea saponin (TS 5 g/kg DM), TS plus disodium fumarate (DF 20 g/kg DM) and coconut oil (7% DM) on methane emissions in sheep using simple box chamber. Eight Huzhou sheep were assigned to four treatments in a 4 × 4 Latin square arrangement. Addition of TS and TSDF decreased (P<0.0001) methane emissions by 8.5 and 9.6%, respectively. Addition of TSDF signifi cantly increased (P<0.01) propionate and decreased (P<0.01) acetate proportion. Addition of TS and TSDF, though not as effectively as coconut oil, can inhibit methane emissions, which is benefi cial for economy and environment.

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“…Changes in CH 4 production usually occur with an alteration in the VFA profile because of alternative electron sinks to dispose of reducing power. Moreover, the transformation of the feed to propionate usually limits the rate of methanogenesis due to the impact of hydrogen transfer (Ungerfeld et al, 2003). The supplementation with sage increased propionate and total VFA concentrations and also altered the A:P ratio, simultaneously inhibiting digestibility in vitro.…”

Section: Discussionmentioning

confidence: 99%

An <i>in vitro</i> study on the effect of sage, <i>Salvia officinalis</i> L., on rumen fermentation

Zmora¹,

Cieślak²,

Pers‐Kamczyc³

et al. 2012

J. Anim. Feed Sci.

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Dried leaves of Salvia officinalis L., a rich source of phytofactors, were evaluated for their effect on methane production, microbial population, and basic parameters of rumen fermentation in a batch culture system. A 400 mg mixture of meadow hay and barley meal at a ratio of 60:40 was used as the substrate. Dried leaves of Salvia officinalis L. were added to the substrate at six levels (4, 10, 20, 40, 100, and 200 mg). A wide range of dried Salvia officinalis L. leaves, in quantities even up to 50% of the substrate, was used to test the effect of increasing amounts of phytofactors in the investigated cultures, mostly on methane production. CH 4 production was significantly mitigated by supplementation with 200 mg of sage per incubation vessel. The supplement affected the protozoa and methanogen populations which indicates that methane emission is associated with these populations; in vitro dry matter digestibility decreased linearly, however. Although Salvia officinalis L. seems to be a plant for which there may be a use in ruminant nutrition and the presented results suggest that the optimal level of Salvia officinalis is between 100 and 200 mg, further investigations under long-term in vivo conditions are necessary, as are the determination of the optimum dose and the active agent.

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Use of some novel alternative electron sinks to inhibit ruminal methanogenesis

Cited by 55 publications

References 28 publications

Redirection of Metabolic Hydrogen by Inhibiting Methanogenesis in the Rumen Simulation Technique (RUSITEC)

Redirection of Metabolic Hydrogen by Inhibiting Methanogenesis in the Rumen Simulation Technique (RUSITEC)

Inhibition of methanogenesis by tea saponin and tea saponin plus disodium fumarate in sheep

An <i>in vitro</i> study on the effect of sage, <i>Salvia officinalis</i> L., on rumen fermentation

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