Use of SSR markers in identification of sunflower isogenic lines in late generations of backcrossing

Dimitrijević, Aleksandra; Imerovski, Ivana; Miladinović, Dragana; Tančić, Sonja; Dušanić, Nenad; Jocić, Siniša; Miklič, Vladimir

doi:10.2298/hel1053191d

Cited by 12 publications

(4 citation statements)

References 16 publications

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“…Different marker types were used for the introgression of Pl 6 into susceptible sunflower material including STS (Sequence-Tagged Sites) markers belonging to the TIR-NBS-LRR class of RGA (Resistance-Gene Analog) ( Bouzidi et al, 2002 ) and co-dominant CAPS (Cleaved Amplified Polymorphic Sequence) markers ( Table 1 ). The developed markers have been successfully used to introduce Pl 6 by MAS or to track the introduction of Pl 6 in backcrosses during conversion of downy mildew susceptible lines into resistant ones ( Dimitrijevic et al, 2010 ; Jocic et al, 2010 ).…”

Section: Marker Development By Linkage Mappingmentioning

confidence: 99%

Sunflower Hybrid Breeding: From Markers to Genomic Selection

Dimitrijević

Horn

2018

Front. Plant Sci.

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In sunflower, molecular markers for simple traits as, e.g., fertility restoration, high oleic acid content, herbicide tolerance or resistances to Plasmopara halstedii, Puccinia helianthi, or Orobanche cumana have been successfully used in marker-assisted breeding programs for years. However, agronomically important complex quantitative traits like yield, heterosis, drought tolerance, oil content or selection for disease resistance, e.g., against Sclerotinia sclerotiorum have been challenging and will require genome-wide approaches. Plant genetic resources for sunflower are being collected and conserved worldwide that represent valuable resources to study complex traits. Sunflower association panels provide the basis for genome-wide association studies, overcoming disadvantages of biparental populations. Advances in technologies and the availability of the sunflower genome sequence made novel approaches on the whole genome level possible. Genotype-by-sequencing, and whole genome sequencing based on next generation sequencing technologies facilitated the production of large amounts of SNP markers for high density maps as well as SNP arrays and allowed genome-wide association studies and genomic selection in sunflower. Genome wide or candidate gene based association studies have been performed for traits like branching, flowering time, resistance to Sclerotinia head and stalk rot. First steps in genomic selection with regard to hybrid performance and hybrid oil content have shown that genomic selection can successfully address complex quantitative traits in sunflower and will help to speed up sunflower breeding programs in the future. To make sunflower more competitive toward other oil crops higher levels of resistance against pathogens and better yield performance are required. In addition, optimizing plant architecture toward a more complex growth type for higher plant densities has the potential to considerably increase yields per hectare. Integrative approaches combining omic technologies (genomics, transcriptomics, proteomics, metabolomics and phenomics) using bioinformatic tools will facilitate the identification of target genes and markers for complex traits and will give a better insight into the mechanisms behind the traits.

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Section: Marker Development By Linkage Mappingmentioning

confidence: 99%

Sunflower Hybrid Breeding: From Markers to Genomic Selection

Dimitrijević

Horn

2018

Front. Plant Sci.

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“…) and SSR (Dimitrijevic et al. ) have been previously used for cluster analyses in sunflower. Our results obtained by cluster analysis corresponded to the phylogenetic relations within the tested lines.…”

Section: Discussionmentioning

confidence: 99%

Identification of PCR markers linked to different Or genes in sunflower

et al. 2012

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Broomrape is a parasitic plant that significantly decreases yield of sunflower. Breeding for resistance has proved to be the most efficient method for suppressing broomrape infestation in the field; however, new races of parasite constantly emerge, and new resistance genes need to be discovered and introduced into cultivated sunflower lines. The aim of this work was to test SSR markers from linkage group 3 (LG3) to investigate whether they could be used for identification of a particular Or gene. Twenty sunflower inbred lines were used, and polymorphism between the lines with various resistance genes and genetic background was investigated. The used markers revealed DNA polymorphism between the investigated lines. Strong association of markers from LG3 with Or 6 , as well as Or 4 and Or 2 genes, was found. Identified markers could be used for introduction of these resistance genes into commercial sunflower lines and for establishment and identification of differential lines.

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“…Single Nucleotide Polymorphism). Они нашли применение в исследованиях генотипической структуры коллекций, сохраняемых в генетических банках семян (Garayalde et al, 2011;Filippi et al, 2015;2020), а также при анализе уровня генетического разнообразия селекционных линий (Sujatha et al, 2008;Darvishzadeh et al, 2010;Dimitrijević et al, 2010;Duca et al, 2013;Zia et al, 2014;Mwangi et al, 2019).…”

Section: Introductionunclassified

Polymorphism of microsatellite markers linked with Rf1 and Pl5/Pl8 loci in sunflower Helianthus annuus L.

Karabitsina

Alpatieva

Kusnetsova

et al. 2022

Biotehnologiâ i selekciâ rastenij

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Background. Microsatellite (SSR) markers are an effective tool for certifying collections of plant genetic resources, as well as for identifying genes that determine valuable biological and agronomic traits. The knowledge of their polymorphism is important for characterizing genetic diversity within the sunflower (Helianthus annuus L.) collection. The present study was aimed at analyzing nucleotide polymorphism of SSR-markers linked with the genes for fertility restoration (Rf1) and downy mildew resistance (Pl5/Pl8). Materials and methods. The material included 84 self-pollinated lines of VIR sunflower genetic collection, F1 and F2 hybrids from crosses between fertile lines VIR 365 and RIL130, and offspring from test crosses. Polymorphism of SSR markers ORS224, ORS511, ORS799 and НА4011 was studied by means of PCR analysis. To determine the microsatellite structure and variability, the amplified fragments were cloned and sequenced. Results. The unique alleles which differed from the typical ones (characteristic for most genotypes) were revealed in the ORS224 marker locus of four lines, and the unique alleles in the ORS511 locus were observed in 10 lines. The ORS511 unique allele of line VIR 365 included two 161 and 240 bp fragments, while line RIL130 was characterized by a typical 159 bp fragment. The profiles of typical and unique markers were inherited as allelic variants of the same locus in F2 of (VIR 365 × RIL130) and a population from VIR 111A × (VIR 365 × RIL130). The nucleotide sequences of unique alleles differed from typical allelic variants in the length and number of repeat units (GA in ORS224 and AT/GT in ORS511), and also by the presence of indels and nucleotide substitutions. Differences in length of НА4011 marker 240 and 200 bp allele variants were caused by 80, 47 and 44 bp indels. Conclusions. A number of lines in the VIR sunflower genetic collection are marked by the unique alleles of microsatellite loci ORS224 and ORS511, which differ from the frequently occurring variants in the length and number of repeat units, as well as in the presence of indels and nucleotide substitutions. Polymorphism of allele variants of HA4011 microsatellite is associated with the presence of indels of 80, 47 and 4 bp.

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Use of SSR markers in identification of sunflower isogenic lines in late generations of backcrossing

Cited by 12 publications

References 16 publications

Sunflower Hybrid Breeding: From Markers to Genomic Selection

Sunflower Hybrid Breeding: From Markers to Genomic Selection

Identification of PCR markers linked to different Or genes in sunflower

Polymorphism of microsatellite markers linked with <i>Rf1</i> and <i>Pl5/Pl8</i> loci in sunflower <i>Helianthus annuus</i> L.

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