Use of stem cells as alternative methods to animal experimentation in predictive toxicology

Kim, Tae Won; Hwan, Jeong; Yun, Jun Won

doi:10.1016/j.yrtph.2019.03.016

Cited by 51 publications

(29 citation statements)

References 186 publications

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“…Stem cell technology, in particular human stem cells, has been transforming the fields of toxicology and pharmacology by providing noncancerous human‐specific cell models for research applications and detection of biologic activity of toxins and pharmaceuticals . While early studies were limited by controversies surrounding the use of embryonic stem cells, discoveries leading to the ability to efficiently re‐program adult human cells into human‐induced pluripotent stem cells (hiPSCs) have led to an explosion in progress in this field.…”

mentioning

confidence: 99%

Botulinum neurotoxins A, B, C, E, and F preferentially enter cultured human motor neurons compared to other cultured human neuronal populations

2019

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Human‐induced pluripotent stem cell (hiPSC)‐derived neurons can be exquisitely sensitive to botulinum neurotoxins (BoNTs), exceeding sensitivity of the traditionally used mouse bioassay. In this report, four defined hiPSC‐derived neuronal populations including primarily GABAergic, glutamatergic, dopaminergic, and motor neurons were examined for BoNT/A, B, C, D, E, and F sensitivity. The data indicate that sensitivity varies markedly for the BoNTs tested. Motor neurons are significantly more sensitive than other neuron types for all BoNTs except BoNT/D. Examination of SNARE protein levels and BoNT‐specific cell surface protein receptors reveals few differences between the cell types except greater expression levels of the receptor protein SV2C and synapsin‐IIa in motor neurons. This indicates that differential toxicity of BoNTs for motor neurons compared to other neuronal cell types involves multiple mechanisms.

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confidence: 99%

Botulinum neurotoxins A, B, C, E, and F preferentially enter cultured human motor neurons compared to other cultured human neuronal populations

2019

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“…Currently, there are several different methodologies to assess the toxicological effects of test chemicals on human cells and animal tissues in vitro and ex vivo . Researchers use 2D cell culture in many assays that have already been validated; however, these methods are limited, as they use nonhuman lineage cells that may have chromosomal anomalies or primary cells that have limitations in the number of passages and the variability between batches [1] . In this context, human stem cells have been explored as a valuable in vitro system for toxicological studies [2] as an alternative to using animals or traditional in vitro methods.…”

Section: Methods Introductionmentioning

confidence: 99%

Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction

et al. 2021

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In vitro stem cell models are used as alternatives to animal models and are important tools for cytotoxicity studies. Researchers can determine the effects of test substances on human cells by evaluating cell viability and differentiation. Here, we describe an in vitro model to quantify adipogenesis based on the Nile red staining of specific lipid droplets and the emission of basic lipids from human adipose tissue-derived mesenchymal stromal cells (AD-MSCs) in the presence of test substances. This assay allows for the prediction of toxicity based on the inhibition of adipogenesis in vitro in a 96-well format. The differentiation of a progenitor cell into a specialized cell, the adipocyte, is easy to monitor and quantify, making this a simple assay. The fluorescence staining of nuclei and lipid droplets is measured after 14 days of cell differentiation to determine cell number and assess cell differentiation using high-content imaging analysis, thus allowing for the identification of chemicals that impact differentiation. We also describe a protocol to assess adipocyte differentiation by fluorescence intensity using a multiplate reader. Researchers can utilize the protocol described here for many purposes to evaluate in vitro adipogenesis. With this method, it is possible to reduce the use of animals.

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“…Las células madre o estromales son células indiferenciadas presentes en el organismo en las etapas embrionarias, fetales y adultas. Estas células darán lugar tras la diferenciación a células específicas que constituyen los tejidos y órganos (1)(2)(3) . Según su origen pueden clasificarse en: células madre embrionarias, células madre fetales, células madre adultas y células madre pluripo-…”

Section: Introductionunclassified

Establecimiento e implementación de un protocolo simplificado de expansión y cultivo de Células Madre de Pulpa Dental Humana (DPSCh)

Francia

Grazioli

Echarte

et al. 2021

odonto

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Use of stem cells as alternative methods to animal experimentation in predictive toxicology

Cited by 51 publications

References 186 publications

Botulinum neurotoxins A, B, C, E, and F preferentially enter cultured human motor neurons compared to other cultured human neuronal populations

Botulinum neurotoxins A, B, C, E, and F preferentially enter cultured human motor neurons compared to other cultured human neuronal populations

Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction

Establecimiento e implementación de un protocolo simplificado de expansión y cultivo de Células Madre de Pulpa Dental Humana (DPSCh)

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