Use of straight vegetable oil mixtures of rape and camelina as on farm fuels in agriculture

Paulsen, Hans; Wichmann, Volker; Schuemann, U.; Richter, Brantlee Spakes

doi:10.1016/j.biombioe.2011.06.031

Cited by 30 publications

(18 citation statements)

References 8 publications

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“…To determine whether acetyl‐TAG produced by expression of Ea DAcT in Camelina possesses reduced viscosity, we measured the kinematic viscosity (according to ASTM D455) of lcTAG purified from wild‐type Camelina oil and of acetyl‐TAG purified from Ea DAcT‐transformed seed oil. The viscosity of lcTAG was 30.6 mm 2 /s, in keeping with the literature values (Bernardo et al ., ; Paulsen et al ., ). In contrast, the kinematic viscosity of acetyl‐TAG was 20.3 mm 2 /s, a 34% reduction.…”

Section: Resultsmentioning

confidence: 97%

Metabolic engineering of oilseed crops to produce high levels of novel acetyl glyceride oils with reduced viscosity, freezing point and calorific value

Liu

Rice

McGlew

et al. 2015

Plant Biotechnology Journal

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SummarySeed oils have proved recalcitrant to modification for the production of industrially useful lipids. Here, we demonstrate the successful metabolic engineering and subsequent field production of an oilseed crop with the highest accumulation of unusual oil achieved so far in transgenic plants. Previously, expression of the Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) gene in wild-type Arabidopsis seeds resulted in the accumulation of 45 mol% of unusual 3-acetyl-1,2-diacyl-sn-glycerols (acetyl-TAGs) in the seed oil (Durrett et al., 2010 PNAS 107:9464). Expression of EaDAcT in dgat1 mutants compromised in their ability to synthesize regular triacylglycerols increased acetyl-TAGs to 65 mol%. Camelina and soybean transformed with the EaDAcT gene accumulate acetyl-triacylglycerols (acetyl-TAGs) at up to 70 mol% of seed oil. A similar strategy of coexpression of EaDAcT together with RNAi suppression of DGAT1 increased acetyl-TAG levels to up to 85 mol% in field-grown transgenic Camelina. Additionally, total moles of triacylglycerol (TAG) per seed increased 20%. Analysis of the acetyl-TAG fraction revealed a twofold reduction in very long chain fatty acids (VLCFA), consistent with their displacement from the sn-3 position by acetate. Seed germination remained high, and seedlings were able to metabolize the stored acetyl-TAGs as rapidly as regular triacylglycerols. Viscosity, freezing point and caloric content of the Camelina acetyl-TAG oils were reduced, enabling use of this oil in several nonfood and food applications.

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Section: Resultsmentioning

confidence: 97%

Metabolic engineering of oilseed crops to produce high levels of novel acetyl glyceride oils with reduced viscosity, freezing point and calorific value

Liu

Rice

McGlew

et al. 2015

Plant Biotechnology Journal

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“…Camelina ( Camelina sativa [L.] Crantz) is a reemerging oilseed crop of the Brassicaceae family and possesses the unique attribute of multiple uses. Among the many uses of camelina, the most frequent focus is on its value as a high potential biofuel feedstock mainly biodiesel and jet fuel (Ciubota‐Rosie, Ruiz, Ramos, & Pérez, ; Keske, Hoag, Brandess, & Johnson, ; Liu, Savas, & Avedisian, ; Paulsen, Wichmann, Schuemann, & Richter, ), and other uses entail several industrial products such as adhesives, resins, hydrophilic monomers, gums, and coatings (Berti, Gesch, Eynck, Anderson, & Cermak, ; Li & Sun, ; Sainger et al, ), a high protein animal feed for different classes of livestock (Adhikari, Heo, & Nyachoti, ; Bullerwell, Collins, Lall, & Anderson, ; Jaśkiewicz, Sagan, & Puzio, ; Pikul et al, ), food and supplements (Berti et al, ) are among the major focus of its cultivation globally. Along with the aforementioned uses, the inherent trait of high oil content (28%–48% on a DM basis) of camelina seed (Bacenetti, Restuccia, Schillaci, & Failla, ; Yang, Caldwell, Corscadden, He, & Li, ) relative to the 26.6%–40.2% oil content of canola (Malhi, Gan, & Raney, ) increases its potential as a non‐conflicting biofuel crop, that is, it has been classified as a minor edible oilseed crop for human food supply compared to canola (Drenth, Olsen, Cabot, & Johnson, ; Koçar, ).…”

Section: Introductionmentioning

confidence: 99%

Nitrogen source and rate effects on grain and potential biodiesel production of camelina in the semiarid environment of northern Nevada

et al. 2018

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The objective of this two-year study (2016-2017 spring) carried out at the University of Nevada, Reno Main Station Field Laboratory, Reno, NV, was to evaluate the effects of nitrogen source, rate, and camelina cultivar on grain yield and potential biodiesel production irrigated with reclaimed water. Treatments were two sources of urea fertilizer [conventional urea (CU) and polymer-coated urea (PCU)], four N rates (0, 40, 80, and 120 kg N ha -1 ), and two cultivars of camelina ("Blaine Creek" and "Pronghorn") arranged in a 4 × 2 × 2 factorial combinations with four replications each in a RCBD experiment. Plot size was 7.6 m long × 1.8 m wide, and camelina was seeded at a rate of 5 kg PLS seed ha -1 .The quantity of light intercepted increased linearly from 44.9% to 65.9% as N application rate increased from 0 to 120 kg N ha -1 , and it was greater for CU (59.6%) compared to PCU (54.0%) fertilized plots. There was a linear increased in grain yield ranging from 534 to 1,010 kg/ha as N application rate increased from 0 to 120 kg N ha -1 . In Year 2, grain yield of Blaine Creek (898 kg/ha) was greater than that of Pronghorn (464 kg/ha). Also, there was a linear increase in estimated biodiesel from 51.2 to 94.2 L/ha as N application rate increased. For both grain and biodiesel production, there was no advantage of using controlledrelease PCU fertilizer and 80 to 120 kg N ha -1 is sufficient for the cultivation of camelina in this environment. Based on the range of grain yield obtained in this study, camelina can be a potential alternative crop to integrate into the annual crop production cycle in water-limited environments like Nevada.

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“…Studies performed with various vegetable oils showed that the direct combustion causes excess of carbonization of the injectors, combustion chamber and the exhaust tubing, and also increase the struggle of the injection, contamination of the lubricant oil, among other problems (KRATZEISEN & MÜLLER, 2010;AGARWAL et al, 2010;PAULSEN et al, 2011;ACHARYA et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Performance of a diesel engine fueled with a preheated blend of soybean oil and petrodiesel

Delalibera

Campolina²,

Neto

et al. 2012

Eng. Agríc.

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Brazil is doing a major effort to find alternatives to diesel oil as combustible. Some study lines are oriented to the development of vegetable oils used as fuel, as a source of getting cheaper and have higher energy density than the converted vegetable oils, and less risk of environmental contamination. The aim of this study was to evaluate the performance, the useful life of the lubricant and some components of a Diesel Cycle engine, with an electronic injection system, in a long-term test operating with a preheated blend (65°C) of 50% (v v -1 ) of soybean oil in petrodiesel. There was a reduction of the useful life of the injectors which presented failure because of high wear with 264 hours of operation and showed an increase in emissions of particulate matter (opacity) which may be assigned to the failures occurred in the injection system. An increase in the useful life of the lubricant, when compared with the literature was also observed. The electronic injection system may favor the burning of the tested fuel. The test was interrupted with 264 hours because of failures in the injection system.

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Use of straight vegetable oil mixtures of rape and camelina as on farm fuels in agriculture

Cited by 30 publications

References 8 publications

Metabolic engineering of oilseed crops to produce high levels of novel acetyl glyceride oils with reduced viscosity, freezing point and calorific value

Metabolic engineering of oilseed crops to produce high levels of novel acetyl glyceride oils with reduced viscosity, freezing point and calorific value

Nitrogen source and rate effects on grain and potential biodiesel production of camelina in the semiarid environment of northern Nevada

Performance of a diesel engine fueled with a preheated blend of soybean oil and petrodiesel

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