Use of TDZ for Micropropagation of Some Mediterranean Crop Species

Mazri, Mouaad Amine; Belkoura, I.; Meziani, Reda

doi:10.1007/978-981-10-8004-3_5

Cited by 5 publications

(2 citation statements)

References 73 publications

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“…Thidiazuron was first reported to have cytokinin activity in 1982. Since then, it has been used successfully in vitro to induce adventitious shoot formation and to promote axillary shoot proliferation [31], in in vitro propagation of plants including medicinal and horticultural crops [32] and for the micropropagation of several plant species [33].…”

Section: Resultsmentioning

confidence: 99%

Effect of Phytohormones on Shoot Proliferation of Doubled Haploid Lines of African Marigold (Tagetes erecta L.)

Mehraj,

Singh,

Kumar

et al. 2024

IJECC

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In vitro propagation of marigold has played a very important role in rapid multiplication of cultivars with desirable traits and production of healthy and disease-free plants. Shoot proliferation is a very important phase of micropropagation. This paper describes an efficient protocol for In vitro micropropagation of Doubled haploid lines of marigold (Tagetes erecta L.) using nodal explants. Murashige and Skoog medium (MS) supplemented with various concentrations and combinations of Benzylaminopurine (BAP), Naphthylacetic acid (NAA), Gibberellic acid (GA3) and Thidiazuron (TDZ) were employed for shoot regeneration in this study, maximum percent sprouting (100%) and minimum days to sprout break (1.48) were found in MS media supplemented with BAP 2mg/L + NAA 0.05mg/L + GA3 1mg/L, while maximum number of sprouts (1.73) and maximum average shoot length (3.98cm) were observed in case of media supplementation with BAP 1mg/L + NAA 0.05mg/L + GA3 0.5mg/L. However, in case of Thidiazuron (TDZ) minimum days taken for shoot proliferation (2.66) and maximum number of shoots per micro shoot (7.04) were observed in media supplemented with BAP 1mg/L + NAA 0.1mg/L + TDZ 0.5mg/L, while maximum length of shoots (4.2cm) was observed in control devoid of any hormones.

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Section: Resultsmentioning

confidence: 99%

Effect of Phytohormones on Shoot Proliferation of Doubled Haploid Lines of African Marigold (Tagetes erecta L.)

Mehraj,

Singh,

Kumar

et al. 2024

IJECC

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“…Most importantly, there is an increase of antioxidant enzymes ( Devireddy et al, 2021 ). Other outcomes include inducing shoot regeneration, roots and morphogenesis in Chinese water chestnut ( Wang et al, 2015 ), in vitro responses such as organogenesis ( Aremu et al, 2015 ; Werbrouck, 2016 ; Chen and Wei, 2018 ; Mazri et al, 2018 ), callus culture bioassays ( Kopecný et al, 2010 ), delayed senescence ( Nisler et al, 2016 ; Prernostava et al, 2018 ) and as basic as increasing yield of the cereal crops ( Ashikari et al, 2005 ; Nisler et al, 2021 ). Moreover, 44% increased zinc levels were present in the seeds of the transgenic barley plant along with drought tolerance.…”

Section: Biotechnological Responsesmentioning

confidence: 99%

Cytokinin Oxygenase/Dehydrogenase Inhibitors: An Emerging Tool in Stress Biotechnology Employed for Crop Improvement

Arora¹,

Sen²

2022

Front. Genet.

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In order to meet the global challenges of food security, one of the foremost solutions lies in enhancing the crop productivity. This can be attained by considering key plant hormones such as cytokinins as agrochemicals as cytokinins in particular are known to control the essential processes of the plants. Even though, it has already been established since 1980s that the enzyme, cytokinin oxidase/dehydrogenase (CKO/CKX) deactivates cytokinins; the potential applications of manipulating these enzymes have mostly been speculated to have a high potential in the biotechnology industry and spreads to agriculture, horticulture and agroforestry. The enzyme is critical in maintaining a balanced level of cytokinins in plants. However, it is yet to be fully established that inhibiting this enzyme can be the constant source of improvement in the productivity of plants, even though success has been obtained in some economically important plant species. Furthermore, the impact efficiency of this enzyme may vary from plant to plant, which needs to be evaluated employing tissue culture and other extrinsic applications. This review intends to cover the relevant studies addressing any biological activity of this enzyme in the current context and any associated biotechnological applications specific to enhanced grain yield, abiotic stress tolerance, delayed senescence and in vitro organogenesis among various plants and not only cereals. Moreover, our study will identify the present gaps in research with respect to many important food crops, which will be useful for researchers who are actively involved in providing a foundation for a variety of genetically improved plants achieved through this manner. In addition to this, other ways of engineering the amount of cytokinin levels appropriate for signaling also needs to be analyzed in order to extend the benefits of cytokinin biology to other crops too. The application of these inhibitors can be considered among the best alternates as well as addition to genetically modified plants for overcoming the gaps in crop demand.

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In Vitro Responses of Some Mediterranean Fruit Crops to Auxin, Cytokinin and Gibberellin Treatments

Mazri

Koufan

Abdelwahd

et al. 2022

Auxins, Cytokinins and Gibberellins Signaling in Plants

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Use of TDZ for Micropropagation of Some Mediterranean Crop Species

Cited by 5 publications

References 73 publications

Effect of Phytohormones on Shoot Proliferation of Doubled Haploid Lines of African Marigold (Tagetes erecta L.)

Effect of Phytohormones on Shoot Proliferation of Doubled Haploid Lines of African Marigold (Tagetes erecta L.)

Cytokinin Oxygenase/Dehydrogenase Inhibitors: An Emerging Tool in Stress Biotechnology Employed for Crop Improvement

In Vitro Responses of Some Mediterranean Fruit Crops to Auxin, Cytokinin and Gibberellin Treatments

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