1998
DOI: 10.1128/jcm.36.6.1584-1587.1998
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Use of Tests for Acidification of Methyl-α- d -Glucopyranoside and Susceptibility to Efrotomycin for Differentiation of Strains of Enterococcus and Some Related Genera

Abstract: A total of 107 Enterococcus strains, 10Vagococcus fluvialis strains, and 8 Lactococcus garvieae strains were tested for acidification of methyl-α-d-glucopyranoside (MGP) and susceptibility to 100-μg efrotomycin (EFRO) disks. All 26 strains ofEnterococcus casseliflavus, including 3 nonmotile and 2 nonpigmented strains, acidified MGP and were resistant to EFRO. All 22 strains of Enterococcus gallinarum, including 5 nonmotile strains, also acidified MGP and were resistant to EFRO. None of the 26 strains of Entero… Show more

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Cited by 35 publications
(12 citation statements)
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“…The identities of the organisms tested and the results of the LM-ARA, MGP, and rapid motility (RM) assays are shown in These results confirm previous observations concerning the utility of MGP acidification for differentiating IVRE from other enterococcal species (3,5). Unfortunately, an overnight incubation step is necessary to obtain adequate sensitivity with the MGP assay.…”
supporting
confidence: 81%
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“…The identities of the organisms tested and the results of the LM-ARA, MGP, and rapid motility (RM) assays are shown in These results confirm previous observations concerning the utility of MGP acidification for differentiating IVRE from other enterococcal species (3,5). Unfortunately, an overnight incubation step is necessary to obtain adequate sensitivity with the MGP assay.…”
supporting
confidence: 81%
“…The ability to accurately differentiate IVRE from other VRE, especially E. faecalis and E. faecium, is nonetheless of considerable importance and, unfortunately, has proven to be somewhat problematic for commercial biochemical identification systems (9,11). A number of tests for rapidly and inexpensively identifying IVRE, including motility (7,15), pigment production (7), acidification of methyl-␣-D-glucopyranoside (MGP) (3,5), and susceptibility to efrotomycin (3), have been described in the literature. A previous study conducted in our laboratory demonstrated that determining the ability of organisms to rapidly reduce litmus milk (LM) and acidify arabinose (ARA) was a useful approach for differentiating between the clinically significant enterococcal species (8).…”
mentioning
confidence: 99%
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“…En la mayoría de los casos, este test necesita incubación a 35º C durante 18 horas, aunque una incubación más prolongada puede ser necesaria en aquellas casos de resultados dudosos. El desarrollo de un color amarillo demuestra una reacción positiva 4 .…”
unclassified
“…La placa se incuba a 35º C durante 18 a 24 horas. Si después de la incubación por 18 horas se observa un área de inhibición alrededor del disco, independiente de su tamaño, el organismo es clasificado como susceptible a la efrotomicina 4 . Hasta el momento, este disco no se encuentra disponible comercialmente y aunque los discos se pueden preparar, este es un test que se utiliza fundamentalmente en investigación (Tabla 1).…”
unclassified