Abstract. Laboratory incubations were performed to estimate nitrification rates and the associated nitrous oxide (N 2 O) emissions under aerobic conditions on a range of soils from National Agricultural Nitrous Oxide Research Program field sites. Significant site-to-site variability in nitrification rates and associated N 2 O emissions was observed under standardised conditions, indicating the need for site-specific model parameterisation. Generally, nitrification rates and N 2 O emissions increased with higher water content, ammonium concentration and temperature, although there were exceptions. It is recommended that site-specific model parameterisation be informed by such data. Importantly, the ratio of N 2 O emitted to net nitrified N under aerobic conditions was small (<0.2% for the majority of measurements) but did vary from 0.03% to 1%. Some models now include variation in the proportion of nitrified N emitted as N 2 O as a function of water content; however, strong support for this was not found across all of our experiments, and the results demonstrate a potential role of pH and ammonium availability. Further research into fluctuating oxygen availability and the coupling of biotic and abiotic processes will be required to progress the process understanding of N 2 O emissions from nitrification.