Use of the amniotic membrane to cover the peritoneal cavity in the reconstruction of the abdominal wall with polypropylene mesh in rats

Abstract

Use of the amniotic membrane to cover the peritoneal cavity in the reconstruction of the abdominal wall with polypropylene mesh in rats Use of the amniotic membrane to cover the peritoneal cavity in Use of the amniotic membrane to cover the peritoneal cavity in Use of the amniotic membrane to cover the peritoneal cavity in Use of the amniotic membrane to cover the peritoneal cavity in Use of the amniotic membrane to cover the peritoneal cavity in the reconstruction of the abdominal wall with polypropylene the reconstruction of the abdominal wall with polypropylene the reconstruction of the abdominal wall with polypropylene the reconstruction of the abdominal wall with polypropylene the reconstruction of the abdominal wall with polypropylene mesh in rats mesh in rats mesh in rats mesh in rats mesh in rats Uso da membrana amniótica como cobertura da cavidade abdominal na Uso da membrana amniótica como cobertura da cavidade abdominal na Uso da membrana amniótica como cobertura da cavidade abdominal na Uso da membrana amniótica como cobertura da cavidade abdominal na Uso da membrana amniótica como cobertura da cavidade abdominal na reconstrução da parede com tela de polipropileno em ratos reconstrução da parede com tela de polipropileno em ratos reconstrução da parede com tela de polipropileno em ratos reconstrução da parede com tela de polipropileno em ratos reconstrução da parede com tela de polipropileno em ratos Objective Objective: to evaluate the efficacy of the amniotic membrane used with polypropylene mesh against the formation of adhesions and its influence on healing. Methods Methods Methods Methods Methods: twenty five female Wistar rats were anesthetized for creating a parietal defect in the anterior abdominal wall. Its correction was made with polypropylene mesh alone and associated with amniotic membrane. In the control group (n=11), the screen was inserted alone. In group A (n=7) we interposed the amniotic membrane between the screen and the abdominal wall. In group B, the amniotic membrane was placed on the mesh, covering it. After seven days, the animals were euthanized for macroscopic and microscopic evaluation of healing. Results Results ResultsResults Results: adhesions were observed in all animals except one in the control group. Severe inflammation was observed in all animals in groups A and B and in three of the control group, with significant difference between them (A and B with p=0.01). Pronounced angiogenic activity was noted in one animal in the control group, six in group A and four in group B, with a significant difference between the control group and group A (p=0.002) and group B (p=0.05). The scar collagen was predominantly mature, except in five animals of the control group, with significant difference between the control group and group A (p=0.05) and group B (p=0.05). Conclusion Conclusion Conclusion Conclusion Conclusion: The amniotic membrane did not alter the formation of adhesions in the first postoperative week. There were also pronounced inflammation, high angiogenic activit...

Effects of the application of the amniotic membrane in the healing process of skin wounds in rats

“…Vascular formation was more significant in the animals that received the HAM, indicating that even its conservation at lower temperatures does not inactivate proangiogenic substances previously identified in the cryopreserved membrane 26 . In addition, the harvesting of the amniotic membrane does not offer any risk to the newborn or the parturient, and the membrane can be easily obtained and stored in laboratories for later use.…”

“…We emphasize that the use of the HAM offers the advantages of high availability, ease of application, and good storability [12][13][14][15][16][17][18] . In this context, it is possible to affirm that by presenting properties that favor the re-structuring of the damaged portions of the tissues, the amniotic membrane could be used as a biological coverage of acute and chronic wounds [18][19][20][21][22][23][24][25][26] .…”

Immediate postoperative evaluation of enteric anastomosis after the use of meloxicam in rats

“…The impact of meloxicam on the inflammatory stage in this study was not significant, perhaps because it is preferential to the inhibition of cyclooxygenase-2, which is believed to be inductive in tissue injuries and at the same time does not inhibit the cicloxigenases, behaving only like a modulating drug 12,38,41,42 .…”

Amniotic Membrane-Coated Polypropylene Mesh for the Repair of Incisional Hernia: An Experimental Study In a Rat Model of Abdominal Wall Defect

“…However, there are limited studies on the use of AM in reconstruction of abdominal wall, all of which were experimental animal studies with conflicting outcomes (6,(25)(26)(27). The majority of these studies showed that AM is an effective antiadhesive barrier in polypropylene mesh repair of abdominal wall hernia (6,25,26). polypropylene mesh alone for the correction of abdominal wall defect in rats.It was found that although AM application did not alter the formation of adhesions after the first week of operation, it accelerated the healing as shown by increased inflammation and angiogenesis activity.…”