Use of the aqueous extract of Eucalyptus microcorys for the treatment in microcosm, of water containing Enterococcus faecalis: hierarchisation of cells' inhibition factors

Abstract

An experimental study in aquatic microcosm was carried out to determine the major factors involved in the inhibition of Enterococcus faecalis in the presence of aqueous extract of Eucalyptus microcorys. The planktonic bacterial cells remained in various concentrations of the aqueous solution at light intensities which fluctuated between 0 and 3,000 lx and incubation periods which ranged from 3 to 24 hours. A hierarchisation of studied factors revealed that the aqueous extract concentration, followed by experimental temperature, light intensity and incubation duration influence the inhibition of E. faecalis cells, respectively, with a rate of 86.82%, 7.03%, 5.25% and 0.90%. The cell abundances dropped significantly at 1.5% (λ ¼ 0.491 and F ¼ 5.518) and 2% (λ ¼ 0.568 and F ¼ 4.055) concentrations coupled with 1,000, 2,000 and 3,000 lx. The highest light intensities and extract concentration produce the highest log removal values. The disinfectant properties of E. microcorys were evaluated by the Chick-Watson model. This Chick-Watson model so obtained varied between log (N/No) ¼ À0.09 Ct and log (N/No) ¼ À0.17 Ct for extract concentrations of 1, 1.5 and 2%. Aqueous extract of E. microcorys could be used for water disinfection.

In Vitro and In Silico Activities of E. radiata and E. cinerea as an Enhancer of Antibacterial, Antioxidant, and Anti-Inflammatory Agents

“…The presence of phytochemicals in plant fractions, such as flavonoids and polyphenols, may be responsible for disruptive bacterial membrane effect [8]. For bacterial illnesses such as sinusitis, sore throat, angina, cough, bronchitis, and urinary tract infections, [46] recommends a decoction of Eucalyptus leaves in water at 10-20 g/L as a daily beverage.…”

Evaluation of the Efficacy of <i>Albizia zygia</i> Extracts on Bacterial Inhibition in Aquatic Microcosm

“…The extract concentrations were 0.5 g/L, 1 g/L and 1.5 g/L. The homogenized extracts were filtered successively on filter paper, sterile cotton, sterile Whatman paper, and finally cellulose nitrate membrane of 0.45 µm porosity (Tamsa et al, 2018).…”

“…Studies have been carried out on the evaluation of the synergistic effect of different pH values and different infusion concentrations of Artemisia annua on Enterococcus faecalis growth in aquatic microcosms under dark and lighting conditions (Mobili et al, 2015). Studies conducted by Tamsa et al (2018) identified the main factors involved in the inhibition of Enterococcus faecalis in the presence of an aqueous extract of Eucalyptus microcorys in an aquatic microcosms. In addition, Metsopkeng et al (2019) evaluated in microcosm the survival of Aeromonas hydrophila and Enteropathogenic Escherichia coli (EPEC), in the presence of Moringa oleifera aqueous seeds extract at concentrations ranging from 1 to 40 g/L, and under incubation temperatures of 4˚C and 23˚C.…”

Effect of aqueous extract of Eucalyptus microcorys on Salmonella typhi strain adhered cells to polythene fragments in water microcosm

“…In general, it has been observed that the inhibition of the growth of bacteria in aquatic environments is linked to the presence of bactericidal and bacteriostatic compounds in plants. 17 The best known for Eucalyptus microcorys are quinones and anthraquinones. It has also been shown in vitro that the essential oils of Eucalyptus microcorys have strong antibacterial activity against Enterococcus faecalis and Salmonella typhi murium.…”