Use of the biodegradable polymer chitosan as a vehicle for applying drugs to the inner ear

Saber, Amanj; Strand, Sabina P.; Ulfendahl, Mats

doi:10.1016/j.ejps.2009.11.003

Cited by 61 publications

(30 citation statements)

References 157 publications

(291 reference statements)

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“…Alternative therapies using molecules such as chitosan could avoid some of the problems associated with antibiotics. Chitosan is a biodegradable molecule that has been used as a drug carrier in some treatment applications [45]. Besides, the antimicrobial activity of chitosan itself has been reported in some studies against a wide range of bacteria [26].…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm and antibacterial effects of specific chitosan molecules on Staphylococcus aureus isolates associated with bovine mastitis

et al. 2017

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Staphylococcus aureus is one of the major pathogens causing bovine intramammary infections (IMIs) and mastitis. Mastitis is the primary cause for the use of antibiotics in dairy farms but therapeutic failure is often observed. One of the reasons for the lack of effectiveness of antibiotic therapy despite the observed susceptibility of bacterial isolates in vitro are bacterial biofilms. In this study, we used chitosan of well-defined molecular weight (0.4–0.6, 1.3, 2.6 and 4.0 kDa) and investigated their antibiofilm and antibacterial activities in in vitro and in vivo models related to S. aureus IMIs. A chitosan of at least 6 units of glucosamine was necessary for maximum antibacterial activity. The 2.6 and 4.0 kDa forms were able to prevent biofilm production by the biofilm hyperproducer strain S. aureus 2117 and a bovine MRSA (methicillin-resistant S. aureus). The intramammary administration of the 2.6 kDa chitosan showed no adverse effects in mice or in cows, as opposed to the slight inflammatory effect observed in mammary glands with the 4.0 kDa derivative. The 2.6 kDa chitosan killed bacteria embedded in pre-established biofilms in a dose-dependent manner with a >3 log10 reduction in CFU at 4 mg/ml. Also, the 2.6 kDa chitosan could prevent the persistence of the internalized MRSA into the mammary epithelial cell line MAC-T. An in vitro checkerboard assay showed that the 2.6 kDa chitosan produced a synergy with the macrolide class of antibiotics (e.g., tilmicosin) and reduced the MIC of both molecules by 2–8 times. Finally, the intramammary administration of the 2.6 kDa chitosan alone (P<0.01) or in combination with tilmicosin (P<0.0001) reduced the colonization of mammary glands in a murine IMI model. Our results suggest that the use of chitosan alone or in combination with a low dose of a macrolide could help reduce antibiotic use in dairy farms.

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Section: Discussionmentioning

confidence: 99%

Antibiofilm and antibacterial effects of specific chitosan molecules on Staphylococcus aureus isolates associated with bovine mastitis

et al. 2017

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“…502 The hearing loss depends on the amount of gel injected in the middle ear (Feng et al, 2014). 503 However, the presence of chitosan (Saber et al, 2010), hyaluronic acid (Martini et al, 1992)or 504 poloxamer 407 hydrogels in the middle eardid not provoke any 505 histological damages to the round window and the cochlea indicating that those hydrogels 506 have no noxious effects by themselves. The hearing loss observed is thus conductive in 507 nature, i.e.…”

Section: Drug Releasementioning

confidence: 98%

“…However, animal studies point out that their efficacy can be strongly dependent on the 538 physicochemical properties of the drug being delivered and polymer characteristics(Wang et 539 al., 2009, Saber et al, 2010, Omotehara et al, 2011, Wang et al, 2011.540 Nanoparticulate systems 541 Nanoparticulate systems, also called nanocarriers, havesizesbelow1 µm and were primarily 542 developed to carry a drug to a target site, thereby avoiding thepotentiallytoxiceffects on 543 healthy tissues. Due to their numerous advantages,nanotechnologies have generated 544 considerable interest in the past several years for drug delivery to the inner ear(Pritz et al, 545 2013, Staecker and Rodgers, 2013).…”

mentioning

confidence: 99%

Recent advances in local drug delivery to the inner ear

Kechai

Agnely

Mamelle

et al. 2015

International Journal of Pharmaceutics

140

111

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“…CS has been selected as coating material because of two reasons (1) CS is a bio-safe and biodegradable polymer (Saber et al 2010;Balamurugan 2012) easily obtained from natural polymer chitin; (2) this polyelectrolyte is positively charged (Schatz et al 2004) at neutral pH, so that CS-capped ZnO will be an ideal material for interacting with negatively charged bio-colloid HA.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of ppm level humic acid in surface water using ZnO–chitosan nano-composite as fluorescence probe

Basumallick

Santra

2015

Appl Water Sci

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Surface water contains natural pollutants humic acid (HA) and fulvic acid at ppm level which form carcinogenic chloro-compounds during chlorination in water treatment plants. We report here synthesis of ZnO-chitosan (CS) nano-composites by simple hydrothermal technique and examined their application potential as fluorescent probe for monitoring ppm level HA. These ZnO-CS composites have been characterized by HRTEM, EDX, FTIR, AFM and Fluorescence Spectra. HRTEM images show the formation of ZnO-CS nano-composites of average diameter of 50-250 nm. Aqueous dispersions of these nano-composites show fluorescence emission at 395 nm when excited at 300 nm which is strongly quenched by ppm level HA indicating their possible use in monitoring ppm level HA present in surface water.

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Use of the biodegradable polymer chitosan as a vehicle for applying drugs to the inner ear

Cited by 61 publications

References 157 publications

Antibiofilm and antibacterial effects of specific chitosan molecules on Staphylococcus aureus isolates associated with bovine mastitis

Antibiofilm and antibacterial effects of specific chitosan molecules on Staphylococcus aureus isolates associated with bovine mastitis

Recent advances in local drug delivery to the inner ear

Monitoring of ppm level humic acid in surface water using ZnO–chitosan nano-composite as fluorescence probe

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