Use of the DCB Technique for Extraction of Hydrous Iron Oxides From Roots of Wetland Plants

Taylor, Gregory J.; Crowder, Adèle A.

doi:10.1002/j.1537-2197.1983.tb12474.x

Cited by 233 publications

(72 citation statements)

References 10 publications

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“…Roots were rinsed three times with deionized water that was added to the DCB extract. The resulting solution was made up to 50 ml with deionized water (Taylor and Crowder 1983). After DCB extraction, roots were oven dried at 70°C for 3 days and weighed.…”

Section: Dcb Extraction Of Iron Plaquementioning

confidence: 99%

A natural rice rhizospheric bacterium abates arsenic accumulation in rice (Oryza sativa L.)

Lakshmanan

Shantharaj

et al. 2015

Planta

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A natural rice rhizospheric isolate abates arsenic uptake in rice by increasing Fe plaque formation on rice roots. Rice (Oryza sativa L.) is the staple food for over half of the world's population, but its quality and yield are impacted by arsenic (As) in some regions of the world. Bacterial inoculants may be able to mitigate the negative impacts of arsenic assimilation in rice, and we identified a nonpathogenic, naturally occurring rice rhizospheric bacterium that decreases As accumulation in rice shoots in laboratory experiments. We isolated several proteobacterial strains from a rice rhizosphere that promote rice growth and enhance the oxidizing environment surrounding rice root. One Pantoea sp. strain (EA106) also demonstrated increased iron (Fe)-siderophore in culture. We evaluated EA106's ability to impact rice growth in the presence of arsenic, by inoculation of plants with EA106 (or control), subsequently grew the plants in As-supplemented medium, and quantified the resulting plant biomass, Fe and As concentrations, localization of Fe and As, and Fe plaque formation in EA106-treated and control plants. These results show that both arsenic and iron concentrations in rice can be altered by inoculation with the soil microbe EA106. The enhanced accumulation of Fe in the roots and in root plaques suggests that EA106 inoculation improves Fe uptake by the root and promotes the formation of a more oxidative environment in the rhizosphere, thereby allowing more expansive plaque formation. Therefore, this microbe may have the potential to increase food quality through a reduction in accumulation of toxic As species within the aerial portions of the plant.

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Section: Dcb Extraction Of Iron Plaquementioning

confidence: 99%

A natural rice rhizospheric bacterium abates arsenic accumulation in rice (Oryza sativa L.)

Lakshmanan

Shantharaj

et al. 2015

Planta

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“…At harvest, rice roots with iron plaque were washed three time with deionized water, and then iron plaque was removed from the surface of the rice roots with 80 ml cold dithionite-citrate-bicarbonate (DCB) (0.03 M sodium citrate and 0.125 M sodium bicarbonate with 0.06 M sodium dithionite) for 60 min at room temperature (20-25°C) (Taylor and Crowder 1983). Roots were rinsed three times with deionized water, and the wash water was added to the DCB-extracts.…”

Section: Measurement Of Dcb Extraction Of Iron Plaque From Rootsmentioning

confidence: 99%

“…Under anaerobic conditions oxygen loss from rice roots induces iron and manganese deposits on the surface of the roots (Armstrong 1967). Iron plaque is commonly formed on the surfaces of roots of wetland plants, including Oryza sativa, Typha latifolia and Phragmites communis, and is mainly caused by the oxidation of ferrous iron and the precipitation of iron oxide on the root surface and rhizosphere (Armstrong 1964;Armstrong 1967;Chen et al 1980;Taylor and Crowder 1983;Taylor et al 1984).…”

Section: Introductionmentioning

confidence: 99%

Iron plaque outside roots affects selenite uptake by rice seedlings (Oryza sativa L.) grown in solution culture

2006

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A hydroponics experiment was conducted to investigate the effects of iron plaque on root surfaces with respect to selenite uptake and translocation within the seedlings of two cultivars of rice (Oryza sativa L. cv Xiushui48 and Bing9652). Different amounts of iron plaque were formed by adding 0, 10, 30, 50, 70 mg Fe l -1 in the nutrient solution. After 24 h of growth, the amount of iron plaque was positively correlated with the Fe 2+ addition to the nutrient solution. These concentrations of Fe, inducing plaque, had no significant effect on the shoot and root growth of rice plants in 50 lg Se l -1 nutrient solution. The amount of Se accumulated in iron plaque was positively correlated to the amount of iron plaque. Increasing iron plaque decreased the selenium concentration in shoots and in roots. At the same time, the translocation of Se from roots to shoots was reduced with increasing amounts of iron plaque. At both the shorter and longer exposure times, the ratio of root-to-shoot selenium was higher than in the controls. More Se stayed in the roots at the longer exposure time than at the shorter time. The concentration of selenium in the xylem sap was sharply decreased with increasing amount of iron plaque on the rice roots. The DCB (dithionite-citrate-bicarbonate)-extracted Se was up to 89.9-91.1% of the total Se when roots with iron plaque (Fe 70) were incubated in 50 lg Se l -1 solution for 30 min. This DCB-extracted Se, however, accounted for only 21.9-28.7% of total Se when roots with iron plaque were incubated in the same solution for 3 days. Se adsorbed in iron plaque can be desorbed by low-molecular-weight organic acids, similar to the desorption of Se from ferrihydrite. These results suggest that iron plaque might act as a 'buffer' for Se in the rhizosphere.

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“…Then the Fe and Mn plaques deposited on the root surface were extracted using the dithionite-citrate-carbonate (DCB) method of Taylor and Crowder (1983b) and McLaughlin et al (1985). The fresh roots were incubated for 3 h at 25°C in 45 mL of a solution (pH 6.5) containing 0.27 M sodium citrate (Na 3 C 6 H 5 O 7 Á2H 2 O) and 0.11 M sodium bicarbonate (NaHCO 3 ), with the addition of 3.0-g sodium dithionite (Na 2 S 2 O 4 ).…”

Section: Cs Treatmentsmentioning

confidence: 99%

Ethylene is involved in the complete-submergence induced increase in root iron and manganese plaques in Oryza sativa

Guo

Gan

et al. 2014

Plant Growth Regul

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Flooding is one of the most important abiotic constraints on rice yields in rain-fed lowlands. During the submergence period, ethylene accumulates rapidly to a physiologically active level in the tissues of submerged plants. Also, iron and manganese plaque formation on roots is an adaptation that allows rice-like plant species to survive in their natural aquatic habitats. This study provides evidence of a time-dependent increase in the iron and manganese plaque content of rice root surfaces during 96 h of complete submergence (CS), accompanied by the increased expression in roots of genes in the OsACS (Os-ACS1, OsACS2, OsACS4 and OsACS5) and OsACO (OsACO1, OsACO2, OsACO3 and OsACO7) families. To further investigate ethylene action in root plaque formation, rice seedlings were completely submerged in Kimura B nutrient solutions containing different concentrations of Fe 2? or Mn 2? . Under these conditions, root plaques were induced dramatically in an almost dose-dependent manner. Iron and manganese plaque formation was promoted by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid and blocked by the ethylene biosynthesis inhibitor aminoethoxyvinyl glycine (AVG) in seedlings grown on media supplemented or not with Fe 2? or Mn 2? . Furthermore, submergence-induced iron and manganese root plaque formation was decreased by pretreatment with 5 lM AVG for 12 h. Our findings suggest that ethylene signaling is involved in CS-induced iron and manganese root plaque formation in rice.

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Use of the DCB Technique for Extraction of Hydrous Iron Oxides From Roots of Wetland Plants

Cited by 233 publications

References 10 publications

A natural rice rhizospheric bacterium abates arsenic accumulation in rice (Oryza sativa L.)

A natural rice rhizospheric bacterium abates arsenic accumulation in rice (Oryza sativa L.)

Iron plaque outside roots affects selenite uptake by rice seedlings (Oryza sativa L.) grown in solution culture

Ethylene is involved in the complete-submergence induced increase in root iron and manganese plaques in Oryza sativa

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