Use of the DCB Technique for Extraction of Hydrous Iron Oxides from Roots of Wetland Plants

Taylor, Gregory J.; Crowder, Adèle A.

doi:10.2307/2443295

Cited by 159 publications

(83 citation statements)

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“…1). Fe has been shown to exert strong competition over other heavy metals for sensitive metabolic sites within the leaves (Kuo 1986;Taylor and Crowder 1983) or in the root tips (Chen et al 2006). Mean shoot Fe concentrations in the present experiment at Fe50 (except at P0) were higher than the 448 mg kg −1 reported for normally grown rice plants (Ottow et al 1982).…”

Section: Discussioncontrasting

confidence: 56%

“…Iron plaque on fresh root surfaces was extracted using a modified dithionite-citrate-bicarbonate (DCB) method (Taylor and Crowder 1983;Otte et al 1989). Briefly, the entire root system of each seedling was incubated for 60 min at 25°C in 60 ml 0.03 mol l −1 sodium citrate (Na 3 C 6 H 5 O 7 · 2H 2 O) and 0.125 mol l −1 sodium bicarbonate (NaHCO 3 ) with the addition of 1.2 g sodium dithionite (Na 2 S 2 O 4 ).…”

Section: Experimental Treatmentsmentioning

confidence: 99%

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Influence of external zinc and phosphorus supply on Cd uptake by rice (Oryza sativa L.) seedlings with root surface iron plaque

et al. 2007

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Section: Discussioncontrasting

confidence: 56%

Section: Experimental Treatmentsmentioning

confidence: 99%

Influence of external zinc and phosphorus supply on Cd uptake by rice (Oryza sativa L.) seedlings with root surface iron plaque

et al. 2007

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“…The corresponding ear samples were washed with deionized water, dried at 70°C for 48 h, and then separated into husk and rice grain using a rice sheller. Iron plaque was extracted from fresh root surfaces using dithionite-citrate-bicarbonate (DCB) solution containing 0.03 M sodium citrate and 0.125 M sodium bicarbonate, with the addition of 0.02 g sodium dithionite per milliliter (Taylor and Crowder 1983;Liu et al 2004). The root sample per cluster was immersed in DCB solution (200 ml) at 25°C for 60 min, and then rinsed three times with deionized water.…”

Section: Sampling and Chemical Analysismentioning

confidence: 99%

Mitigating heavy metal accumulation into rice (Oryza sativa L.) using biochar amendment — a field experiment in Hunan, China

Zheng

Chen

Cai

et al. 2015

Environ Sci Pollut Res

138

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A field experiment was conducted to investigate the effect of bean stalk (BBC) and rice straw (RBC) biochars on the bioavailability of metal(loid)s in soil and their accumulation into rice plants. Phytoavailability of Cd was most dramatically influenced by biochars addition. Both biochars significantly decreased Cd concentrations in iron plaque (35-81 %), roots (30-75 %), shoots (43-79 %) and rice grain (26-71 %). Following biochars addition, Zinc concentrations in roots and shoots decreased by 25.0-44.1 and 19.9-44.2 %, respectively, although no significant decreases were observed in iron plaque and rice grain. Only RBC significantly reduced Pb concentrations in iron plaque (65.0 %) and roots (40.7 %). However, neither biochar significantly changed Pb concentrations in rice shoots and grain. Arsenic phytoavailability was not significantly altered by biochars addition. Calculation of hazard quotients (HQ) associated with rice consumption revealed RBC to represent a promising candidate to mitigate hazards associated with metal(loid) bioaccumulation. RBC reduced Cd HQ from a 5.5 to 1.6. A dynamic factor's way was also used to evaluate the changes in metal(loid) plant uptake process after the soil amendment with two types of biochar. In conclusion, these results highlight the potential for biochar to mitigate the phytoaccumulation of metal(loid)s and to thereby reduce metal(loid) exposure associated with rice consumption.

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“…Arsenic speciation was separated by a PRP-X100 anion exchange HPLC column (150×4.1 mm, Hamilton, Netherlands) under the solution of 7.0 mmol L −1 (NH 4 ) 2 HPO 4 and 7.0 mmol L −1 NH 4 NO 3 (pH=6.2). Roots were incubated in DCB (dithionite-citrate-bicarbonate) solution for the extraction of iron plaques on the root surface (Taylor and Crowder 1983;Liu et al 2004). Arsenic concentration in the iron plaque extraction solution was determined by ICP-MS (Agilent 7500, Agilent Technologies, USA).…”

Section: Determination Of Fe 2+ S 2− and As Concentrationsmentioning

confidence: 99%

Arsenic bioavailability to rice plant in paddy soil: influence of microbial sulfate reduction

2015

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Purpose High arsenic (As) mobility in anaerobic paddy soil due to microbial Fe-and As-reducing processes results in As accumulation into rice plants. Sulfur (S) also undergoes microbial reducing processes in the anaerobic paddy soil, while this process interacts with the Fe-and As-reducing processes, forms secondary minerals, and thus influences As mobility in paddy soil. This work was carried out to investigate the role of sulfur and sulfate-reducing bacteria (SRB) in As redox transformation and bioavailability to rice plant in an anaerobic paddy soil. Materials and methods Anaerobic incubation experiments with or without sulfate (SO 4 2− ) and SRB were carried out to monitor S and iron (Fe) dynamics and their relations to As speciation and release to soil solution. Rice cultivation in pot experiment was then carried out to check the SO 4 2− amendment in bioavailability and uptake into rice plants.Results and discussion The inoculation of an enriched SRB community into the sterilized paddy soil increased reduction and release of As to the soil solution after flooding, showing its ability in arsenate (As(V)) as well as SO 4 2− reduction to arsenite (As(III)) and sulfide (S 2− ), respectively. Sulfate addition (2 and 100 mM) into the anaerobic paddy soil increased the reduction of SO 4 2− and ferric iron (Fe 3+ ) and resulted to lower dissolved As in the soil solution, which limited As mobility in the paddy soil. Application of SO 4 2− (100 mg kg −1 ) into paddy soil finally decreased the concentration of dissolved As in the soil solution by 23.5 % and also decreased As content in rice roots and iron plaque by 22.6 and 30.5 %, respectively. Conclusions The results revealed the important role of sulfur and the SRB activity in As speciation and mobility in anaerobic paddy soil, implying a lower As bioavailability to rice plants under sulfur-enriched anaerobic paddy soil, and an efficient way to reduce As accumulation in rice plants by sulfur fertilization in paddy soils.

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Use of the DCB Technique for Extraction of Hydrous Iron Oxides from Roots of Wetland Plants

Cited by 159 publications

References 0 publications

Influence of external zinc and phosphorus supply on Cd uptake by rice (Oryza sativa L.) seedlings with root surface iron plaque

Influence of external zinc and phosphorus supply on Cd uptake by rice (Oryza sativa L.) seedlings with root surface iron plaque

Mitigating heavy metal accumulation into rice (Oryza sativa L.) using biochar amendment — a field experiment in Hunan, China

Arsenic bioavailability to rice plant in paddy soil: influence of microbial sulfate reduction

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