Use of the dynamic gastro-intestinal model TIM to explore the survival of the yogurt bacterium Streptococcus thermophilus and the metabolic activities induced in the simulated human gut

Uriot, Ophélie; Galia, Wessam; Awussi, Ahoefa Ablavi; Perrin, Clarisse; Denis, Sylvain; Chalancon, Sandrine; Lorson, Emilie; Poirson, Chantal; Junjua, Maira; Roux, Yves Le; Alric, Monique; Dary, Annie; Blanquet‐Diot, Stéphanie; Roussel, Yvonne

doi:10.1016/j.fm.2015.05.007

Cited by 32 publications

(27 citation statements)

References 60 publications

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“…5 ). However, further studies in vivo should be done, because the food matrix usually confers extra protection on bacterial survival in the stomach 61 .…”

Section: Discussionmentioning

confidence: 99%

Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice

Hui

Guo

Zhang

et al. 2018

Sci Rep

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Human exposure to lead mainly occurs by ingestion of contaminated food, water and soil. Blocking lead uptake in the gastrointestinal tract is a novel prevention strategy. Whole-cell biosorbent for lead was constructed with PbrR genetically engineered on the cell surface of Escherichia coli (E. coli), a predominant strain among intestinal microflora, using lipoprotein (Lpp)-OmpA as the anchoring protein. In vitro, the PbrR displayed cells had an enhanced ability for immobilizing toxic lead(II) ions from the external media at both acidic and neutral pH, and exhibited a higher specific adsorption for lead compared to other physiological two valence metal ions. In vivo, the persistence of recombinant E. coli in the murine intestinal tract and the integrity of surface displayed PbrR were confirmed. In addition, oral administration of surface-engineered E. coli was safe in mice, in which the concentrations of physiological metal ions in blood were not affected. More importantly, lead associated with PbrR-displayed E. coli was demonstrated to be less bioavailable in the experimental mouse model with exposure to oral lead. This is reflected by significantly lower blood and femur lead concentrations in PbrR-displayed E. coli groups compared to the control. These results open up the possibility for the removal of toxic metal ions in vivo using engineered microorganisms as adsorbents.

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“…5 ). However, further studies in vivo should be done, because the food matrix usually confers extra protection on bacterial survival in the stomach 61 .…”

Section: Discussionmentioning

confidence: 99%

Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice

Hui

Guo

Zhang

et al. 2018

Sci Rep

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“…In order to increase the bioavailability and systemic health effect, it was stated that new processing developments should be considered to increase the free FA in the grain matrix (Anson et al 2009). Uriot et al (2016) aimed to determine the survival and adaptation capacity of 30 S. thermophillus strains from the yoghurt bacteria in the intestinal region in terms of urease, low temperature shock protein and amino acid decarboxylase enzymes. The survivability status of 4 strains was determined by TIM.…”

Section: Tim-1 Model Systemmentioning

confidence: 99%

“…In addition, the bacterial cells of the LMD9 strain were transported in fermented milk, leading to a better development in fermented products than non-fermented products. In addition, recombinant In Vivo Expression Technology (RIVET), when applied to the LMD9 strain, showed that the hisS enzyme (producing tRNA) present in such species exhibited activity in the artificial gastric environment (Uriot et al 2016). Miszczycha et al (2014), in another study, aimed to determine shiga toxin producing E. coli in contaminated raw cheeses by TIM.…”

Section: Tim-1 Model Systemmentioning

confidence: 99%

TIM Systems: A Novel Approach for Determination of Bioaccessibility and Bioavailability of Food Components

Çağlar

Demırci

2018

European Journal of Science and Technology

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The increasing rate of consciousness on innovative changes in foods has been increasingly grew the demand for the information about metabolism of these foods. Data about bioaccessibility and bioavailability of food components become more vital for both consumers and producers in the context of developing new healthier foods. Both nutrition and food researchers try to develop methods that allow user to obtain sufficient results of bioaccessibility and bioavailability of food compounds. Therefore, this study will summarize the TIM system, a novel system that mimics the human gastrointestinal system by all means, including working principles and studies performed in this system. In vivo and in vitro studies cannot be used as useful tool for measuring bioaccessibility and bioavailability of food components because of the physiological differences between human and animal. TIM studies were shown that this novel system could be easily used for the monitoring gastrointestinal movements of foods. In conclusion, TIM systems provide adequate data including digestibility, bioaccessibility and bioavailability of many different food products. By mimicking the GI tract, TIM system is a promising tool for the investigation of digestion, absorption components and health promoting aspects of foods.

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“…Streptococcus thermophilus IFFI 6038 (IFFI 6038) is a key species involved in acidophilus milk as well as providing texture to various fermented dairy products. A previous study indicated that S. thermophilus could help patients with lactose intolerance attributed to the degradation function of microbial β‐galactosidase (Uriot and others ). In addition, various researchers found that IFFI 6038 also functioned in oxidation resistance and immune adjustment (Ito and others ; Ogita and others ; Uriot and others ).…”

Section: Introductionmentioning

confidence: 99%

“…A previous study indicated that S. thermophilus could help patients with lactose intolerance attributed to the degradation function of microbial β‐galactosidase (Uriot and others ). In addition, various researchers found that IFFI 6038 also functioned in oxidation resistance and immune adjustment (Ito and others ; Ogita and others ; Uriot and others ). However, the viability of IFFI 6038 is threatened by diverse environmental factors, such as temperature and oxygen, when it is introduced into food.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Acid‐Resistant Microcapsules with Shell‐Matrix Structure to Enhance Stability of Streptococcus Thermophilus IFFI 6038

Zhou

Chen

et al. 2017

Journal of Food Science

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Microencapsulation is an effective technology used to protect probiotics against harsh conditions. Extrusion is a commonly used microencapsulation method utilized to prepare probiotics microcapsules that is regarded as economical and simple to operate. This research aims to prepare acid-resistant probiotic microcapsules with high viability after freeze-drying and optimized storage stability. Streptococcus thermophilus IFFI 6038 (IFFI 6038) cells were mixed with trehalose and alginate to fabricate microcapsules using extrusion. These capsules were subsequently coated with chitosan to obtain chitosan-trehalose-alginate microcapsules with shell-matrix structure. Chitosan-alginate microcapsules (without trehalose) were also prepared using the same method. The characteristics of the microcapsules were observed by measuring the freeze-dried viability, acid resistance, and long-term storage stability of the cells. The viable count of IFFI 6038 in the chitosan-trehalose-alginate microcapsules was 8.34 ± 0.30 log CFU g after freeze-drying (lyophilization), which was nearly 1 log units g greater than the chitosan-alginate microcapsules. The viability of IFFI 6038 in the chitosan-trehalose-alginate microcapsules was 6.45 ± 0.09 log CFU g after 120 min of treatment in simulated gastric juices, while the chitosan-alginate microcapsules only measured 4.82 ± 0.22 log CFU g . The results of the long-term storage stability assay indicated that the viability of IFFI 6038 in chitosan-trehalose-alginate microcapsules was higher than in chitosan-alginate microcapsules after storage at 25 °C. Trehalose played an important role in the stability of IFFI 6038 during storage. The novel shell-matrix chitosan-trehalose-alginate microcapsules showed optimal stability and acid resistance, demonstrating their potential as a delivery vehicle to transport probiotics.

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Use of the dynamic gastro-intestinal model TIM to explore the survival of the yogurt bacterium Streptococcus thermophilus and the metabolic activities induced in the simulated human gut

Cited by 32 publications

References 60 publications

Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice

Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice

TIM Systems: A Novel Approach for Determination of Bioaccessibility and Bioavailability of Food Components

Preparation of Acid‐Resistant Microcapsules with Shell‐Matrix Structure to Enhance Stability of Streptococcus Thermophilus IFFI 6038

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