Use of the enzyme-linked immunosorbent assay for detection of antibodies toChlamydia trachomatis

Duc-Goiran, P.; Raymond, Josette; Leaute, J.‐B.; Orfila, J

doi:10.1007/bf02019920

Cited by 4 publications

(1 citation statement)

References 23 publications

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“…Several tests utilizing broadly cross-reactive antigen (usually of the LGV serotypes) in an enzyme-linked immunosorbent assay (ELISA) have been described for the detection of antibodies to C. trachomatis (36,44,79,94,157). These tests generally detect genus antibodies, using purified EBs or RBs.…”

Section: Serologic Methodsmentioning

confidence: 99%

Laboratory diagnosis of human chlamydial infections

Barnes

1989

Clin Microbiol Rev

167

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Chlamydia trachomatis is a human pathogen that causes ocular disease (trachoma and inclusion conjunctivitis), genital disease (cervicitis, urethritis, salpingitis, and lymphogranuloma venereum), and respiratory disease (infant pneumonitis). Respiratory chlamydioses also occur with infection by avian strains of C. psittaci or infection by the newly described TWAR agent. Diagnosis of most acute C. trachomatis infections relies on detection of the infecting agent by cell culture, fluorescent antibody, immunoassay, cytopathologic, or nucleic acid hybridization methods. Individual non-culture tests for C. trachomatis are less sensitive and specific than the best chlamydial cell culture system but offer the advantages of reduced technology and simple transport of clinical specimens. Currently available nonculture tests for C. trachomatis perform adequately as screening tests in populations in which the prevalence of infection is greater than 10%. A negative culture or nonculture test for C. trachomatis does not, however, exclude infection. The predictive value of a positive nonculture test may be unsatisfactory when populations of low infection prevalence are tested. Tests that detect antibody responses to chlamydial infection have limited utility in diagnosis of acute chlamydial infection because of the high prevalence of persistent antibody in healthy adults and the cross-reactivity due to infection by the highly prevalent C. trachomatis and TWAR agents. Assays for changes in antibody titer to the chlamydial genus antigen are used for the diagnosis of respiratory chlamydioses. A single serum sample that is negative for chlamydial antibody excludes the diagnosis of lymphogranuloma venereum.

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Section: Serologic Methodsmentioning

confidence: 99%

Laboratory diagnosis of human chlamydial infections

Barnes

1989

Clin Microbiol Rev

167

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Chlamydiae

Smith¹

1985

Laboratory Procedures in Clinical Microbiology

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Enzyme-linked immunosorbent assay using three different antigen preparations for detection of antibodies toChlamydia trachomatis

Raymond

Duc-Goiran

Joundy

et al. 1985

Eur. J, Clin. Microbiol.

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The ability of an enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Chlamydia trachomatis was evaluated in 100 sera using three different antigen preparations as substrates (sonicated organisms, Triton X solubilized antigen and SDS solubilized antigen). The results were compared to those obtained by a standard microimmunofluorescence assay. The results obtained by the three ELISA techniques and the microimmunofluorescence method were in relatively good agreement (76%); some discrepant results were observed in sera with a low antibody titer. There was good agreement of results obtained by the three ELISA techniques (84%). The microimmunofluorescence method showed the greatest sensitivity. Assuming the microimmunofluorescence method accurately demonstrates antibodies, the ELISA using Triton X solubilized antigen showed the highest degree of specificity (97%), and the ELISA with sonicated organisms the greatest sensitivity (82%) and accuracy (86%).

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Use of the enzyme-linked immunosorbent assay for detection of antibodies toChlamydia trachomatis

Cited by 4 publications

References 23 publications

Laboratory diagnosis of human chlamydial infections

Laboratory diagnosis of human chlamydial infections

Chlamydiae

Enzyme-linked immunosorbent assay using three different antigen preparations for detection of antibodies toChlamydia trachomatis

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